Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint

Pei Wang; Jiang Zhu; Xi-liang Xie; Ming-he Sui; Qiu-ju Zhang; Wen-rui Jia; Si-yuan Xin; Yang Liu; Zhong-wei Hou

doi:10.1007/s11655-015-2157-7

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Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint

Updated：2021-08-27

- Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint
- Chinese Journal of Integrative Medicine Vol. 22, Issue 7, Pages: 532-536(2016)
- Affiliations：
  
  1. School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine,Beijing,China
  2. The Key Unit of Evaluation of Characteristic Acupuncture Therapy, State Administration of Traditional Chinese Medicine,Beijing,China
  3. The Research Institute of Acupuncture and Moxibustion in Houma City, Houma,Shanxi Province,China
  4. School of Preclinical Medicine, Beijing University of Chinese Medicine,Beijing,China
  5. Chengde Medical College,Hebei Province,Chengde,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81202760) and the Doctoral Program of Higher Educition of Ministry Education of China (No. 20100013120014)
- DOI：10.1007/s11655-015-2157-7
  CLC：
- Published：2016，
  
  Published Online：11 August 2015，
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Wang, P., Zhu, J., Xie, Xl. et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint., Chin. J. Integr. Med. 22, 532–536 (2016). https://doi.org/10.1007/s11655-015-2157-7

Pei Wang, Jiang Zhu, Xi-liang Xie, et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint. [J]. Chinese Journal of Integrative Medicine 22(7):532-536(2016)
Wang, P., Zhu, J., Xie, Xl. et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint., Chin. J. Integr. Med. 22, 532–536 (2016). https://doi.org/10.1007/s11655-015-2157-7 DOI：

Pei Wang, Jiang Zhu, Xi-liang Xie, et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint. [J]. Chinese Journal of Integrative Medicine 22(7):532-536(2016) DOI： 10.1007/s11655-015-2157-7.

摘要

To investigate the effect of direct moxibustion at Ganshu (BL18) on the serum concentrations of tumor specific growth factor (TSGF) and tumor necrosis factor α (TNF-α) in a rat model with precancerous lesion of primary hepatocellular carcinoma (HCC)

so as to explore the mechanism of moxibustion underlying improvement of HCC. Sixty male Wistar rats were randomly divided into control group (n=10)

model group (n=20)

prevention group 1 (n=15) and prevention group 2 (n=15). The normal rats were injected with physiological saline as blank control. At the same time

the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibustion with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1 (1 treatment course

20 days) and prevention group 2 (2 treatment courses

40 days)

5 doses for each acupoint

0.5 mg/dose

once every other day. At each time point (before model establishment

the end of 1st course prevention

the end of 2nd course prevention and the end of model establishment)

serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay. Compared with the control group

there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment (P<0.05). At the end of the 1st course of direct moxibustion

the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group (P<0.05). At the end of the 2nd course of direct moxibustion

serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference (P<0.05)

and the levels of TSGF and TNF-α in the prevention group 2 were significantly reduced in comparison with the model group (P<0.05). It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis

and the therapeutic effect of two courses were better than one course.

Abstract

so as to explore the mechanism of moxibustion underlying improvement of HCC. Sixty male Wistar rats were randomly divided into control group (n=10)

model group (n=20)

prevention group 1 (n=15) and prevention group 2 (n=15). The normal rats were injected with physiological saline as blank control. At the same time

20 days) and prevention group 2 (2 treatment courses

40 days)

5 doses for each acupoint

0.5 mg/dose

once every other day. At each time point (before model establishment

the end of 1st course prevention

the end of 2nd course prevention and the end of model establishment)

serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay. Compared with the control group

there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment (P<0.05). At the end of the 1st course of direct moxibustion

the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group (P<0.05). At the end of the 2nd course of direct moxibustion

serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference (P<0.05)

and the therapeutic effect of two courses were better than one course.

关键词

direct moxibustionDiethylnitrosaminehepatocellular carcinomatumor specific growth factortumor necrosis factor αChinese Medicine

Keywords

direct moxibustionDiethylnitrosaminehepatocellular carcinomatumor specific growth factortumor necrosis factor αChinese Medicine

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