Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy

Ling Qu; Hong Zhang; Bei Gu; Wei Dai; Qun-li Wu; Lian-qing Sun; Li Zhao; Yue Shi; Xiao-chun Liang

doi:10.1007/s11655-015-2164-8

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Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy

Updated：2021-08-27

- Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy
- Chinese Journal of Integrative Medicine Vol. 22, Issue 3, Pages: 185-192(2016)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
  2. Cell Center, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
  3. Department of Electron Microscope, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of China (No. 30572438), the Beijing Science Nuture Foundation (No. 7132189), Science Foundation of Peking Union Medical College Hospital (No. 2013-098) and Youth Foundation of Peking Union Medical College Hospital
- DOI：10.1007/s11655-015-2164-8
  CLC：
- Published：2016，
  
  Published Online：31 March 2015，
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Qu, L., Zhang, H., Gu, B. et al. Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy., Chin. J. Integr. Med. 22, 185–192 (2016). https://doi.org/10.1007/s11655-015-2164-8

Ling Qu, Hong Zhang, Bei Gu, et al. Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy. [J]. Chinese Journal of Integrative Medicine 22(3):185-192(2016)
Qu, L., Zhang, H., Gu, B. et al. Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy., Chin. J. Integr. Med. 22, 185–192 (2016). https://doi.org/10.1007/s11655-015-2164-8 DOI：

Ling Qu, Hong Zhang, Bei Gu, et al. Jinmaitong (筋脉通) alleviates the diabetic peripheral neuropathy by inducing autophagy. [J]. Chinese Journal of Integrative Medicine 22(3):185-192(2016) DOI： 10.1007/s11655-015-2164-8.

摘要

To observe the deregulation of autophagy in diabetic peripheral neuropathy (DPN) and investigate whether Jinmaitong (筋脉通

JMT) alleviates DPN by inducing autophagy. DPN models were established by streptozotocin-induced diabetic rats and Schwann cells (SCs) cultured in high glucose medium. The pathological morphology was observed by the improved Bielschowsky's nerve fiber axonal staining and the Luxol fast blue-neutral red myelin staining. The ultrastructure was observed by the transmission electron microscopy. Beclin1 level was detected by immunohistochemistry and Western blot. The proliferation of cultured SCs was detected by methylthiazolyldiphenyl-tetrazolium bromide. Diabetic peripheral nerve tissues demonstrated pathological morphology and reduced autophagic structure

accompanied with down-regulation of Beclin1. JMT apparently alleviated the pathological morphology change and increased the autophagy [in vivo

Beclin1 integral optical density (IOD) value of the control group 86.6±17.7

DM 43.9±8.8

JMT 73.3 ±17.8

P<0.01 or P<0.05

in vitro Beclin1 IOD value of the glucose group 0.47±0.25 vs the control group 0.88±0.29

P<0.05]. Consequently

inhibition of autophagy by 3-methyladenine resulted in a time- and concentrationdependent decrease of the proliferation of SCs (P<0.05

P<0.01). Down-regulation of autophagy in SCs might contribute to the pathogenesis of DPN. JMT alleviates diabetic peripheral nerve injury at least in part by inducing autophagy.

Abstract

To observe the deregulation of autophagy in diabetic peripheral neuropathy (DPN) and investigate whether Jinmaitong (筋脉通

accompanied with down-regulation of Beclin1. JMT apparently alleviated the pathological morphology change and increased the autophagy [in vivo

Beclin1 integral optical density (IOD) value of the control group 86.6±17.7

DM 43.9±8.8

JMT 73.3 ±17.8

P<0.01 or P<0.05

in vitro Beclin1 IOD value of the glucose group 0.47±0.25 vs the control group 0.88±0.29

P<0.05]. Consequently

inhibition of autophagy by 3-methyladenine resulted in a time- and concentrationdependent decrease of the proliferation of SCs (P<0.05

P<0.01). Down-regulation of autophagy in SCs might contribute to the pathogenesis of DPN. JMT alleviates diabetic peripheral nerve injury at least in part by inducing autophagy.

关键词

diabetic peripheral neuropathySchwann cellsautophagyBeclin1JinmaitongChinese Medicine

Keywords

diabetic peripheral neuropathySchwann cellsautophagyBeclin1JinmaitongChinese Medicine

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Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Translational Medicine Center, Chinese Academy of Medical Sciences

Georg-August-Universität Göttingen, Göttingen

The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics’ Ministry of Public Health

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