L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway

Bin-rui Yang; Nan Yu; Yan-hui Deng; Pui Man Hoi; Bin Yang; Guang-yu Liu; Wei-hong Cong; Simon Ming-Yuen Lee

doi:10.1007/s11655-015-2165-7

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L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway

Updated：2021-08-27

- L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway
- Chinese Journal of Integrative Medicine Vol. 21, Issue 5, Pages: 361-368(2015)
- Affiliations：
  
  1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao,China
  2. Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the grants from the Science and Technology Development Fund, Macau SAR (No. 078/2005/A2) and the Science and Technology Plan of Beijing (No. Z141100002214011)
- DOI：10.1007/s11655-015-2165-7
  CLC：
- Published：2015，
  
  Published Online：17 March 2015，
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Yang, Br., Yu, N., Deng, Yh. et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway., Chin. J. Integr. Med. 21, 361–368 (2015). https://doi.org/10.1007/s11655-015-2165-7

Bin-rui Yang, Nan Yu, Yan-hui Deng, et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. [J]. Chinese Journal of Integrative Medicine 21(5):361-368(2015)
Yang, Br., Yu, N., Deng, Yh. et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway., Chin. J. Integr. Med. 21, 361–368 (2015). https://doi.org/10.1007/s11655-015-2165-7 DOI：

Bin-rui Yang, Nan Yu, Yan-hui Deng, et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. [J]. Chinese Journal of Integrative Medicine 21(5):361-368(2015) DOI： 10.1007/s11655-015-2165-7.

摘要

To investigate whether I-tetrahydropalmatine (I-THP)

an alkaloid mainly present in Corydalis family

could ameliorate early vascular inflammatory responses in atherosclerotic processes. Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs)

which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs. L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore

I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation. These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B.

Abstract

To investigate whether I-tetrahydropalmatine (I-THP)

an alkaloid mainly present in Corydalis family

could ameliorate early vascular inflammatory responses in atherosclerotic processes. Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs)

关键词

I-tetrahydropalmatineatherosclerosiscell adhesion moleculestumor necrosis factor-αnuclear factor-κ B

Keywords

I-tetrahydropalmatineatherosclerosiscell adhesion moleculestumor necrosis factor-αnuclear factor-κ B

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Department of Traditional Chinese Medicine, Shanghai No. 6 People’s Hospital, Affiliated to Shanghai Jiaotong University

Beijing Institute of Traditional Chinese Medicine

Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University

Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou

Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University

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