Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats

Jie Bao; Zhi-jun Xie; Lei-ming Chen; Jing Sun; Yong-sheng Fan

doi:10.1007/s11655-015-2281-4

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Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats

Updated：2021-08-27

- Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats
- Chinese Journal of Integrative Medicine Vol. 22, Issue 12, Pages: 902-909(2016)
- Affiliations：
  
  1. Laboratory of Rheumatoid Immune, The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Zhejiang Xinhua Hospital,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the Traditional Chinese Medicine Scientific Research Fund of Zhejiang Province, China (No. 2013ZA063 and No. 2012ZB062), the Key Scientific and Technological Innovation Team Plan of Zhejiang Province, China (No. 2009R50042-002) and the Third Level of 151 Talents Project of Zhejiang Province, China
- DOI：10.1007/s11655-015-2281-4
  CLC：
- Published：2016，
  
  Published Online：11 August 2015，
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Bao, J., Xie, Zj., Chen, Lm. et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats., Chin. J. Integr. Med. 22, 902–909 (2016). https://doi.org/10.1007/s11655-015-2281-4

Jie Bao, Zhi-jun Xie, Lei-ming Chen, et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats. [J]. Chinese Journal of Integrative Medicine 22(12):902-909(2016)
Bao, J., Xie, Zj., Chen, Lm. et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats., Chin. J. Integr. Med. 22, 902–909 (2016). https://doi.org/10.1007/s11655-015-2281-4 DOI：

Jie Bao, Zhi-jun Xie, Lei-ming Chen, et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats. [J]. Chinese Journal of Integrative Medicine 22(12):902-909(2016) DOI： 10.1007/s11655-015-2281-4.

摘要

To determine the effective dosage and formulation of agkistrodon in collagen-induced arthritis (CIA) rats. CIA was induced by injection of collagen in complete/incomplete Freund’s adjuvant. Agkistrodon decoction

agkistrodon powder

and agkistrodon wine were administered daily starting from the onset of arthritis. Paw swelling degree was measured by using a volume-measuring instrument every 7 days after primary immunization. Arthritis index was measured and calculated using the “five scoring method” every 7 days. The levels of serum interleukin-1ß (IL-1ß) and type II collagen IgG antibodies were detected by enzyme-linked immunosorbent assay. Finally

all ankles were removed

and X-ray radiography was performed with In-vivo Imaging System FX. Samples were counterstained with hematoxylin and eosin for analysis. Among the various dosage formulations of agkistrodon

high-dose powder

which was equivalent to an amount of 6 g/day in adults

showed better effects on the inhibition of joint swelling and reduction of arthritis index score. The relatively low levels of serum IL-1 and anti-type II collagen IgG antibodies

as well as the X-ray radiography and pathology results

further proved the superiority of the high-dose powder over the other formulations. The effect of decoction on inhibiting joint swelling was inversely proportional to the dosage. Other effects

such as reduction of arthritis index score and the levels of serum IL-1 and anti-type II collagen IgG antibodies

were directly proportional to the dosage. While the use of large dose agkistrodon wine led to negative effects. These data highlight the potential function of high-dose agkistrodon powder

which was equivalent to an amount of 6 g/day in adults. The powder can quickly relieve the symptoms of rheumatoid arthritis and prevent aggravation of disease

especially during the early period.

Abstract

agkistrodon powder

all ankles were removed

and X-ray radiography was performed with In-vivo Imaging System FX. Samples were counterstained with hematoxylin and eosin for analysis. Among the various dosage formulations of agkistrodon

high-dose powder

which was equivalent to an amount of 6 g/day in adults

showed better effects on the inhibition of joint swelling and reduction of arthritis index score. The relatively low levels of serum IL-1 and anti-type II collagen IgG antibodies

as well as the X-ray radiography and pathology results

further proved the superiority of the high-dose powder over the other formulations. The effect of decoction on inhibiting joint swelling was inversely proportional to the dosage. Other effects

such as reduction of arthritis index score and the levels of serum IL-1 and anti-type II collagen IgG antibodies

were directly proportional to the dosage. While the use of large dose agkistrodon wine led to negative effects. These data highlight the potential function of high-dose agkistrodon powder

which was equivalent to an amount of 6 g/day in adults. The powder can quickly relieve the symptoms of rheumatoid arthritis and prevent aggravation of disease

especially during the early period.

关键词

rheumatoid arthritisagkistrodoncollagen-induced arthritisdifferent dosage formsChinese Medicine

Keywords

rheumatoid arthritisagkistrodoncollagen-induced arthritisdifferent dosage formsChinese Medicine

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Department of Rheumatism Immunity, First Affiliated Hospital, Anhui University of Chinese Medicine

Anhui University of Chinese Medicine

Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine

The Fifth People’s Hospital of Yuhang District of Hangzhou

School of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine

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