In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study

Fan Lin; Bin-ling Chen; Yi-zheng Wang; Dong Gao; Jun Song; T. J. Kaptchuk; Ke-ji Chen

doi:10.1007/s11655-015-2289-9

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In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study

Updated：2021-08-27

- In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study
- Chinese Journal of Integrative Medicine Vol. 24, Issue 8, Pages: 606-612(2018)
- Affiliations：
  
  1. College of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. The Second Hospital of Longyan,Fujian Province,Longyan,China
  3. Experimental Research Center, China Academy of Chinese Medical Sciences,Beijing,China
  4. Beth Israel Deaconess Medical Center, Harvard Medical School,Boston,USA
  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 81072933, 81173431 and 81102844);T. J. Kaptchuk was partially supported by National Center for Complementary and Alternative Medicine, National Institutes of Health, USA (No. 2K24 AT004095)
- DOI：10.1007/s11655-015-2289-9
  CLC：
- Published：2018，
  
  Published Online：14 August 2015，
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Lin, F., Chen, Bl., Wang, Yz. et al. In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study., Chin. J. Integr. Med. 24, 606–612 (2018). https://doi.org/10.1007/s11655-015-2289-9

Fan Lin, Bin-ling Chen, Yi-zheng Wang, et al. In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study. [J]. Chinese Journal of Integrative Medicine 24(8):606-612(2018)
Lin, F., Chen, Bl., Wang, Yz. et al. In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study., Chin. J. Integr. Med. 24, 606–612 (2018). https://doi.org/10.1007/s11655-015-2289-9 DOI：

Fan Lin, Bin-ling Chen, Yi-zheng Wang, et al. In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study. [J]. Chinese Journal of Integrative Medicine 24(8):606-612(2018) DOI： 10.1007/s11655-015-2289-9.

摘要

To compare the angiogenesis behaviors of vascular endothelial growth factor (VEGF) and Chinese medicine Xuefu Zhuyu Decoction (血府逐瘀汤

XZD) treatments. Human microvascular endothelial cells (HMEC-1) were treated with various concentrations of either XZD-containing serum (XZD-CS) or VEGF for 24

and 72 h

respectively. Cell viability

proliferation

migration

adhesion

and in vitro tube formation assays were used to assess their angiogenic effects. VEGF promoted all cellular phases involved in angiogenesis including cell viability

proliferation

migration

adhesion

and tube formation (<0.05 or <0.01). Unlike the continuous promotion effects of VEGF at the above stages

XZD inhibited cell viability and proliferation (<0.05 or <0.01) and only promoted tube formation in the early phase of angiogenesis (<0.01). These two medications promote different angiogenesis behaviors

which might be an important reason for their distinct therapeutic profile in clinical usage.

Abstract

To compare the angiogenesis behaviors of vascular endothelial growth factor (VEGF) and Chinese medicine Xuefu Zhuyu Decoction (血府逐瘀汤

XZD) treatments. Human microvascular endothelial cells (HMEC-1) were treated with various concentrations of either XZD-containing serum (XZD-CS) or VEGF for 24

and 72 h

respectively. Cell viability

proliferation

migration

adhesion

and in vitro tube formation assays were used to assess their angiogenic effects. VEGF promoted all cellular phases involved in angiogenesis including cell viability

proliferation

migration

adhesion

and tube formation (<0.05 or <0.01). Unlike the continuous promotion effects of VEGF at the above stages

which might be an important reason for their distinct therapeutic profile in clinical usage.

关键词

Xuefu Zhuyu DecoctionChinese Medicinevascular endothelial growth factorangiogenesis

Keywords

Xuefu Zhuyu DecoctionChinese Medicinevascular endothelial growth factorangiogenesis

references

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Department of Cardiovascular, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Department of Shang Han Teaching and Research, Hubei University of Traditional Chinese Medicine

Department of Immunology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Department of Molecular Biology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Traditional Chinese Medicine Research and Development Center, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

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