Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease

Yong Wang; Chun Li; Hong Chang; Ling-hui Lu; Qi Qiu; Yu-lin Ouyang; Jun-da Yu; Shu-zhen Guo; Jing Han; Wei Wang

doi:10.1007/s11655-015-2401-1

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Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease

Updated：2021-08-27

- Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease
- Chinese Journal of Integrative Medicine Vol. 22, Issue 8, Pages: 597-604(2016)
- Affiliations：
  
  1. Precilincal School, Beijing University of Chinese Medicine,Beijing,China
  2. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine,Beijing,China
  3. Capital Medical University Beijing Anzhen Hospital,Beijing,China
  4. Southern Illinois University School of Medicine,Springfield,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 81202788, 81473456, 81470191 and 81302908), the National Science and Technology Pillar Program (No. 2012BAI29B07), Beijing Natural Science Foundation (No. 7142099), and Excellent Young Scientist Foundation of Beijing University of Chinese Medicine (No. 2015-JYB-XYQ001)
- DOI：10.1007/s11655-015-2401-1
  CLC：
- Published：2016，
  
  Published Online：16 May 2016，
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Wang, Y., Li, C., Chang, H. et al. Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease., Chin. J. Integr. Med. 22, 597–604 (2016). https://doi.org/10.1007/s11655-015-2401-1

Yong Wang, Chun Li, Hong Chang, et al. Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease. [J]. Chinese Journal of Integrative Medicine 22(8):597-604(2016)
Wang, Y., Li, C., Chang, H. et al. Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease., Chin. J. Integr. Med. 22, 597–604 (2016). https://doi.org/10.1007/s11655-015-2401-1 DOI：

Yong Wang, Chun Li, Hong Chang, et al. Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease. [J]. Chinese Journal of Integrative Medicine 22(8):597-604(2016) DOI： 10.1007/s11655-015-2401-1.

摘要

To investigate the underlying metabolomic profifiling of coronary heart disease (CHD) with blood stasis syndrome (BSS). CHD model was induced by a nameroid constrictor in Chinese miniature swine. Fifteen miniature swine were randomly divided into a model group (n=9) and a control group (n=6)

respectively according to arandom number table. After 4 weeks

plasma hemorheology was detected by automatic hemorheological analyzer

indices including hematocrit

plasma viscosity

blood viscosity

rigidity index and erythrocyte sedimentation rate; cardiac function was assessed by echocardiograph to detect left ventricular end-systolic diameter (LVED)

left ventricular end-diastolic diameter (LVEDd)

ejection fraction (EF)

fractional shortening (FS) and other indicators. Gas chromatography coupled with mass spectrometry (GC-MS) and bioinformatics were applied to analyze spectra of CHD plasma with BSS. The results of hemorheology analysis showed signifificant changes in viscosity

with low shear whole blood viscosity being lower and plasma viscosity higher in the model group compared with the control group. Moreover

whole blood reduction viscosity at high shear rate and whole blood reduction viscosity at low shear rate increased signifificantly (P <0.05). The echocardiograph results demonstrated that cardiac EF and FS showed signifificant difference (P <0.05)

with EF values being decreased to 50% or less. The GC-MS data showed that principal component analysis can clearly separate the animals with BSS from those in the control group. The enriched Kyoto Encyclopedia of Genes and Genomes biological pathways results suggested that the patterns involved were associated with dysfunction of energy metabolism including glucose and lipid disorders

especially in glycolysis/gluconeogenesis

galactose metabolism and adenosine-triphosphate-binding cassette transporters. Glucose metabolism and lipid metabolism disorders were the major contributors to the syndrome classifification of CHD with BSS.

Abstract

respectively according to arandom number table. After 4 weeks

plasma hemorheology was detected by automatic hemorheological analyzer

indices including hematocrit

plasma viscosity

blood viscosity

rigidity index and erythrocyte sedimentation rate; cardiac function was assessed by echocardiograph to detect left ventricular end-systolic diameter (LVED)

left ventricular end-diastolic diameter (LVEDd)

ejection fraction (EF)

with low shear whole blood viscosity being lower and plasma viscosity higher in the model group compared with the control group. Moreover

especially in glycolysis/gluconeogenesis

关键词

metabolomic profilingBlood Stasis Syndromecoronary heart diseaseChinese Medicine

Keywords

metabolomic profilingBlood Stasis Syndromecoronary heart diseaseChinese Medicine

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College of Health Information Technology and Management, Shanghai University of Medicine and Health Sciences

Department of Cardiologic Medicine, The Second People’s Hospital of Fujian Province

The Second Clinic of Chinese Medicine, Fujian University of Traditional Chinese Medicine

Department of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Department of Cardiology, Guang’anmen Hospital

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