Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

Yong-sen Jia; Xue-qin Hu; Ji-an Li; Szasz Andras; Gabriella Hegyi; Bing-sheng Han

doi:10.1007/s11655-016-2096-3

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Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

Updated：2021-08-27

- Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells
- Chinese Journal of Integrative Medicine Vol. 22, Issue 5, Pages: 384-389(2016)
- Affiliations：
  
  1. Basic Section, College of Chinese Medicine, North China University of Science and Technology,Hebei Province,Tangshan,China
  2. Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University,Hangzhou,China
  3. Oncotherm Innovation and Trade Ltd., Pecs,Hungary
  4. Acupuncture Rehabilitation Foundation,Budapest,Hungary
  5. Traditional Chinese Medicine Hospital of Shenzhen,Guangdong Province,Shenzhen,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Fundation of Hebei Province of China (No. H2013209053), Scientific and Technological Project of Administration of Traditional Chinese Medicine of Hebei Province, China (No. 2014185)
- DOI：10.1007/s11655-016-2096-3
  CLC：
- Published：2016，
  
  Published Online：5 March 2016，
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Jia, Ys., Hu, Xq., Li, Ja. et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells., Chin. J. Integr. Med. 22, 384–389 (2016). https://doi.org/10.1007/s11655-016-2096-3

Yong-sen Jia, Xue-qin Hu, Ji-an Li, et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells. [J]. Chinese Journal of Integrative Medicine 22(5):384-389(2016)
Jia, Ys., Hu, Xq., Li, Ja. et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells., Chin. J. Integr. Med. 22, 384–389 (2016). https://doi.org/10.1007/s11655-016-2096-3 DOI：

Yong-sen Jia, Xue-qin Hu, Ji-an Li, et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells. [J]. Chinese Journal of Integrative Medicine 22(5):384-389(2016) DOI： 10.1007/s11655-016-2096-3.

摘要

To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤

TLD) on esophageal carcinoma Eca109 cells. Eca109 cells were treated with TLD and its separated formulae

including the clearing-heat and detoxification formula (Q)

activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide assay

cell morphology was observed using a microscope

the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF-κB) signal pathway was detected by Western blot. The half maximal inhibitory concentrations of TLD

Q and H were 386

771 and 729 mg/L

respectively. TLD

Q and H significantly inhibited cell proliferation

with 69.43%

60.84% and 61.90% of treated cells in the G phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD

and H compared with the control group (P<0.05)

and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group (P>0.05). Western blot detection indicated slight differences in the inhibition of the NF-κB pathway by the different formulae. TLD formula strongly inhibited IKKβ

NF-κB

interleukin-6 and tumor necrosis factor-α expression compared with the control group. TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF-κB transduction cascade. The combination of the herbs found in the three separate formulae

Q and Z

work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.

Abstract

To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤

TLD) on esophageal carcinoma Eca109 cells. Eca109 cells were treated with TLD and its separated formulae

including the clearing-heat and detoxification formula (Q)

activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide assay

cell morphology was observed using a microscope

the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF-κB) signal pathway was detected by Western blot. The half maximal inhibitory concentrations of TLD

Q and H were 386

771 and 729 mg/L

respectively. TLD

Q and H significantly inhibited cell proliferation

with 69.43%

60.84% and 61.90% of treated cells in the G phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD

and H compared with the control group (P<0.05)

NF-κB

Q and Z

work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.

关键词

esophageal carcinomaEca109 cells Tonglian Decoctionproliferationcell cyclenuclear factor-kappa BChinese Medicine

Keywords

esophageal carcinomaEca109 cells Tonglian Decoctionproliferationcell cyclenuclear factor-kappa BChinese Medicine

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Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Complex Prescription

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