Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model

Huan-huan Luo; Feng-xue Zhang; Wei Wu; Xin-hua Wang

doi:10.1007/s11655-016-2097-2

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Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model

Updated：2021-08-27

- Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model
- Chinese Journal of Integrative Medicine Vol. 22, Issue 10, Pages: 768-773(2016)
- Affiliations：
  
  1. Journal-Publishing Center, Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Department of Tropical Medicine, Guangzhou University of Chinese Medicine,Guangzhou,China
  3. Dean’s Office, Guangdong Provincial Hospital of Chinese Medicine,Guangzhou,China
  4. Headmaster’s Office, Guangzhou Medical University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation for Distinguished Young Scholars of China (No. 81102535)
- DOI：10.1007/s11655-016-2097-2
  CLC：
- Published：2016，
  
  Published Online：5 May 2016，
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Luo, Hh., Zhang, Fx., Wu, W. et al. Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model., Chin. J. Integr. Med. 22, 768–773 (2016). https://doi.org/10.1007/s11655-016-2097-2

Huan-huan Luo, Feng-xue Zhang, Wei Wu, et al. Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model. [J]. Chinese Journal of Integrative Medicine 22(10):768-773(2016)
Luo, Hh., Zhang, Fx., Wu, W. et al. Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model., Chin. J. Integr. Med. 22, 768–773 (2016). https://doi.org/10.1007/s11655-016-2097-2 DOI：

Huan-huan Luo, Feng-xue Zhang, Wei Wu, et al. Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model. [J]. Chinese Journal of Integrative Medicine 22(10):768-773(2016) DOI： 10.1007/s11655-016-2097-2.

摘要

To evaluate the effect of Chinese medicine Haoqin Qingdan Decoction (蒿芩清胆汤

HQD) for febrile disease dampness-heat syndrome (FDDHS). Forty mice were divided into four groups

including normal control

FDDHS (induced by Radix et Rhizoma Rhei recipe and influenza virus A1 FM1 model)

HQD

and the ribavirin groups (10 in each). The normal control and FDDHS groups were administered normal saline. HQD and the ribavirin groups were administered HQD and ribavirin intragastrically once daily at a dose of 64 g/(kg d) and 0.07 g/(kg d)

respectively for 7 days. Lethargy

rough hair

diarrhea

tongue color and sole color were evaluated for pathological changes in morphology. The tongue and lung tissues were collected for histology. The CD14 and toll-like receptor 4 (TLR4) expression levels were measured using real-time quantitative polymerase chain reaction. More than 80% of the FDDHS mice showed hypokinesia and lethargy

and pathological changes associated with rough hair

diarrhea

tongue color and sole color. With advanced treatment for 7 days

the thick greasy tongue fur of the HQD and ribavirin groups were thinner than that of the FDDHS group (P<0.05)

and it was the thinnest in the ribavirin group as compared with that in other groups (P<0.05). The CD14 and TLR4 expression levels in the lung tissues of HQD and ribavirin groups significantly delined compared with the model group (P<0.05 or P<0.01). CD14 was down-regulated more remarkably in the HQD group compared with the ribavirin group (P<0.05)

whereas the converse was true with TLR4 (P<0.05). We established a FDDHS mouse model showing systemic clinical symptoms. Both HQD and ribavirin can inhibit the expression of CD14 and TLR4 in FDDHS mice

while the effect of ribavirin might be much more violent. The expression changes of CD14 and TLR4 consistently refers to lipopolysaccharide

the commonly and hotly inducing factor in FDDHS.

Abstract

To evaluate the effect of Chinese medicine Haoqin Qingdan Decoction (蒿芩清胆汤

HQD) for febrile disease dampness-heat syndrome (FDDHS). Forty mice were divided into four groups

including normal control

FDDHS (induced by Radix et Rhizoma Rhei recipe and influenza virus A1 FM1 model)

HQD

respectively for 7 days. Lethargy

rough hair

diarrhea

and pathological changes associated with rough hair

diarrhea

tongue color and sole color. With advanced treatment for 7 days

the thick greasy tongue fur of the HQD and ribavirin groups were thinner than that of the FDDHS group (P<0.05)

while the effect of ribavirin might be much more violent. The expression changes of CD14 and TLR4 consistently refers to lipopolysaccharide

the commonly and hotly inducing factor in FDDHS.

关键词

CD14toll-like receptor 4febrile disease dampness-heat syndromeHaoqin Qingdan DecoctionribavirinChinese Medicine

Keywords

CD14toll-like receptor 4febrile disease dampness-heat syndromeHaoqin Qingdan DecoctionribavirinChinese Medicine

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