FOLLOWUS
Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice
- Chinese Journal of Integrative Medicine Vol. 22, Issue 11, Pages: 840-845(2016)
- Affiliations：
1. Department of Pharmacy, Hospital of People’s Liberation Army,Beijing,China,302
2. Pharmacy College, Chengdu University of Traditional Chinese Medicine,Chengdu,China
3. Animal Laboratory Center, Hospital of People’s Liberation Army,Beijing,China,302
4. Key Lab of Modern Preparation of Traditimal Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine,Nanchang,China
- Author bio：
- Funds：Supported by the National Key New Drugs Innovation Foundation (No. 2009ZX09103-349) and the Beijing Natural Science Foundation (No. 7122176)
DOI：10.1007/s11655-016-2254-2
CLC：Published：2016，
Published Online：26 October 2016，
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Jin, Sy., Li, Rs., Shen, Bd. et al. Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice., Chin. J. Integr. Med. 22, 840–845 (2016). https://doi.org/10.1007/s11655-016-2254-2
Shi-ying Jin, Rui-sheng Li, Bao-de Shen, et al. Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice. [J]. Chinese Journal of Integrative Medicine 22(11):840-845(2016)
Jin, Sy., Li, Rs., Shen, Bd. et al. Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice., Chin. J. Integr. Med. 22, 840–845 (2016). https://doi.org/10.1007/s11655-016-2254-2 DOI：
Shi-ying Jin, Rui-sheng Li, Bao-de Shen, et al. Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice. [J]. Chinese Journal of Integrative Medicine 22(11):840-845(2016) DOI： 10.1007/s11655-016-2254-2.
摘要
To ascertain anti-fatigue constituents and mechanisms of Herpetospermum caudigerum. The 80% ethanol extracts of Herpetospermum caudigerum were partitioned with chloroform
ethyl acetate and n-butanol
respectively. Male Kunming mice were divided into 13 groups with 16 mice in each group: a control group fed with water
9 groups treated with 3 fractions of Herpetospermum caudigerum (chloroform fraction
ethyl acetate fraction and n-butanol fraction) at dose of 80
160 and 320 mg/kg for the low-dose group
medium-dose group and high-dose group
3 herpetrione (HPE) treated groups fed with HPE at dose of 15
30
and 60 mg/kg for the low-dose group
medium-dose group and high-dose group. All animals were treated once per day for 30 days. Anti-fatigue activity was assessed through the forced swimming test and serum biochemical parameters including blood lactic acid (BLA)
blood urea nitrogen (BUN)
malondialdehyde (MDA)
hepatic glycogen (HG)
lactic dehydrogenase (LDH)
superoxide dismutase (SOD) and glutathione peroxidase (GPx) determined following the recommended procedures provided by the commercial kits. Compared with the control group
the lignans extract (ethyl acetate fraction) of Herpetospermum caudigerum and HPE could signifificantly prolonged the exhaustive swimming time (P<0.05 or P<0.01)
and also increased the HG levels (P<0.05 or P<0.01) and the activities of antioxidant enzymes (SOD
GPx and LDH
P<0.05 or P<0.01); BLA and MDA levels were decreased considerably in lignans extract and HPE treated groups (P<0.05 or P<0.01). HPE also could significantly decrease the BUN contents compared with the control group (P<0.05). The chloroform and n-butanol fraction showed no effect on swimming time and biochemical parameters. The lignans extract had antifatigue activities and HPE may be partly responsible for the anti-fatigue effects of Herpetospermum caudigerum. The possible mechanisms of anti-fatigue activity were related to the decrease of BUN and BLA
the increase of the HG storage and protecting corpuscular membrane by preventing lipid oxidation via modifying several enzyme activities.
Abstract
To ascertain anti-fatigue constituents and mechanisms of Herpetospermum caudigerum. The 80% ethanol extracts of Herpetospermum caudigerum were partitioned with chloroform
ethyl acetate and n-butanol
respectively. Male Kunming mice were divided into 13 groups with 16 mice in each group: a control group fed with water
9 groups treated with 3 fractions of Herpetospermum caudigerum (chloroform fraction
ethyl acetate fraction and n-butanol fraction) at dose of 80
160 and 320 mg/kg for the low-dose group
medium-dose group and high-dose group
3 herpetrione (HPE) treated groups fed with HPE at dose of 15
30
and 60 mg/kg for the low-dose group
medium-dose group and high-dose group. All animals were treated once per day for 30 days. Anti-fatigue activity was assessed through the forced swimming test and serum biochemical parameters including blood lactic acid (BLA)
blood urea nitrogen (BUN)
malondialdehyde (MDA)
hepatic glycogen (HG)
lactic dehydrogenase (LDH)
superoxide dismutase (SOD) and glutathione peroxidase (GPx) determined following the recommended procedures provided by the commercial kits. Compared with the control group
the lignans extract (ethyl acetate fraction) of Herpetospermum caudigerum and HPE could signifificantly prolonged the exhaustive swimming time (P<0.05 or P<0.01)
and also increased the HG levels (P<0.05 or P<0.01) and the activities of antioxidant enzymes (SOD
GPx and LDH
P<0.05 or P<0.01); BLA and MDA levels were decreased considerably in lignans extract and HPE treated groups (P<0.05 or P<0.01). HPE also could significantly decrease the BUN contents compared with the control group (P<0.05). The chloroform and n-butanol fraction showed no effect on swimming time and biochemical parameters. The lignans extract had antifatigue activities and HPE may be partly responsible for the anti-fatigue effects of Herpetospermum caudigerum. The possible mechanisms of anti-fatigue activity were related to the decrease of BUN and BLA
the increase of the HG storage and protecting corpuscular membrane by preventing lipid oxidation via modifying several enzyme activities.
关键词
Herpetospermum caudigerumlignansherpetrioneanti-fatigue activity
Keywords
Herpetospermum caudigerumlignansherpetrioneanti-fatigue activity
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