Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats

Qi Wang; Hao Li; Fei-xue Wang; Lei Gao; Ji-chang Qin; Jian-gang Liu; Yun Wei; Mei-xia Liu

doi:10.1007/s11655-016-2255-1

Your Location：

Home >

Browse articles >

Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats

Updated：2021-08-27

- Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats
- Chinese Journal of Integrative Medicine Vol. 23, Issue 9, Pages: 672-680(2017)
- Affiliations：
  
  1. Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Graduate School, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81173383) and Beijing Municipal Science and Technology Plan (No. Z141100002214003);Conflict of Interest;We declare that we have no conflict of interest.;Author Contributions;Li H and Liu JG contributed to experimental design and supervising the whole experimental process;Gao L, Qin JC, Wei Y and Liu MX were involved in the experimental conducting;Wang Q and Wang FX contributed to the data analysis and manuscript preparation. All the authors read, revised and approved the final manuscript.
- DOI：10.1007/s11655-016-2255-1
  CLC：
- Published：2017，
  
  Published Online：29 March 2016，
Scan for full text
Wang, Q., Li, H., Wang, Fx. et al. Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats., Chin. J. Integr. Med. 23, 672–680 (2017). https://doi.org/10.1007/s11655-016-2255-1

Qi Wang, Hao Li, Fei-xue Wang, et al. Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats. [J]. Chinese Journal of Integrative Medicine 23(9):672-680(2017)
Wang, Q., Li, H., Wang, Fx. et al. Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats., Chin. J. Integr. Med. 23, 672–680 (2017). https://doi.org/10.1007/s11655-016-2255-1 DOI：

Qi Wang, Hao Li, Fei-xue Wang, et al. Huannao Yicong Decoction (还脑益聪方) extract reduces inflammation and cell apoptosis in Aβ1-42-induced Alzheimer's disease model of rats. [J]. Chinese Journal of Integrative Medicine 23(9):672-680(2017) DOI： 10.1007/s11655-016-2255-1.

摘要

Huannao Yicong Decoction (还脑益聪方

HYD)

an effective herbal formula against Alzheimer's disease (AD)

has been proven to have neuroprotective action in amyloid β-protein1-42 (Aβ1-42)-induced rat model. This study was designed to characterize mechanisms by which HYD leads to suppression of inflflammation and apoptosis in the brains of Aβ1-42-induced rat. A total of 72 rats were divided into 6 groups

which were referred to as: sham operation group

model group

donepezil-treated group

HYD low-dose group (HYDL)

HYD middle-dose group (HYDM) and HYD high-dose group (HYDH). Rats in HYDL

HYDM and HYDH were injected with Aβ1-42 at the CA1 region of hippocampus to form AD model and were fed the HYD extract at different dose of 3.78

7.56 and 18.90 g crude drug/kg. The behavioral changes of rats were evaluated by Morris water maze (MWM) before sacrififice. Pathological changes of the brain tissue were evaluated using hematoxylin eosin (HE) staining. The levels of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) were measured by radioimmunoassay. The levels of Aβ and proteins that are associated with apoptosis such as B-cell lymphoma-2 protein (Bcl-2)

Bcl-2-associated X protein (Bax)

cysteine-aspartic protease (caspase)-3

-8

-9 and -12 in serum were measured by immunohistochemistry. Compared with the sham operation group

the spatial learning and memory abilities of AD rats were signifificantly decreased (P<0.05 or P<0.01; Expressions of IL-1

TNF-α

Aβ and apoptosis-signaling proteins caspase-3

-8

-9

-12 were signifificantly up-regulated (P<0.05 or P<0.01). The ratio of Bcl-2 to Bax were signifificantly decreased in the model group (P<0.01). When treated with HYD extract

the spatial learning and memory abilities of AD-model rats were significantly increased (P<0.05 or P<0.01)

IL-1

TNF-α

Aβ

caspase-3

-8

-9 and -12 were down-regulated (P<0.05 or P<0.01)

and the ratio of Bcl-2 to Bax were reduced (P<0.05 or P<0.01). HYD extract can improve the learning and memory ability defificits

alleviate the inflflammatory response and pathological manifestations induced by Aβ1-42 injection in the rat model of AD. HYD down-regulates the levels of IL-1

TNF-α and Aβ

and decreases the rate of apoptosis by modulating apoptosis-signaling-related proteins such as caspase-3

-8

-9

and -12.

Abstract

Huannao Yicong Decoction (还脑益聪方

HYD)

an effective herbal formula against Alzheimer's disease (AD)

which were referred to as: sham operation group

model group

donepezil-treated group

HYD low-dose group (HYDL)

HYD middle-dose group (HYDM) and HYD high-dose group (HYDH). Rats in HYDL

HYDM and HYDH were injected with Aβ1-42 at the CA1 region of hippocampus to form AD model and were fed the HYD extract at different dose of 3.78

Bcl-2-associated X protein (Bax)

cysteine-aspartic protease (caspase)-3

-8

-9 and -12 in serum were measured by immunohistochemistry. Compared with the sham operation group

the spatial learning and memory abilities of AD rats were signifificantly decreased (P<0.05 or P<0.01; Expressions of IL-1

TNF-α

Aβ and apoptosis-signaling proteins caspase-3

-8

-9

-12 were signifificantly up-regulated (P<0.05 or P<0.01). The ratio of Bcl-2 to Bax were signifificantly decreased in the model group (P<0.01). When treated with HYD extract

the spatial learning and memory abilities of AD-model rats were significantly increased (P<0.05 or P<0.01)

IL-1

TNF-α

Aβ

caspase-3

-8

-9 and -12 were down-regulated (P<0.05 or P<0.01)

and the ratio of Bcl-2 to Bax were reduced (P<0.05 or P<0.01). HYD extract can improve the learning and memory ability defificits

alleviate the inflflammatory response and pathological manifestations induced by Aβ1-42 injection in the rat model of AD. HYD down-regulates the levels of IL-1

TNF-α and Aβ

and decreases the rate of apoptosis by modulating apoptosis-signaling-related proteins such as caspase-3

-8

-9

and -12.

关键词

Alzheimer's diseaseapoptosisinflammationHuannao Yicong DecoctionChinese medicine compound

Keywords

Alzheimer's diseaseapoptosisinflammationHuannao Yicong DecoctionChinese medicine compound

references

Li H, Cai LL, Liu JG, Liu LT, Guan J, Liu MF, et al. Effect of early intervention with extract of Huannao Yicong Decoction on the pathologic picture of hippocampus and neurocyte apoptosis in APP transgenic mice model of dementia. Chin J Inter Med 2011;17:430–435.

Li H, Liu MF, Liu JG, Liu LT, Guan J. Effects of Huannao Yicong Recipe extract on the learning and memory ability and related factors of Aβ generation in the brain of APP transgenic mice. Chin J Integr Tradit West Med (Chin) 2013;33:90–94.

Liu MX, Liu JG, Li H, Liu LT, Liang L, Hu J, et al. The effect and mechanism of Huannao Yicong Decoction extract on learning and memory ability in APP/PS1 mouse. Chin J Pharmacol Toxicol (Chin) 2014;28:10–17.

Lu YZ, Li H, Liu JG, Bi PY, Wei Y. Simultaneous and sensitive quantification of seven active compounds in Huan-Nao-Yi-Cong-Fang and rat serum by UFLC-ESI/MS. Anal Methods 2014;6:5727–5733.

Paxions G, Watson C, eds. The rat brain in stereotaxic coordinates. 6th ed. San Diego: Academic Press; 2008:52–84.

Beiko J, Lander R, Hampson E, Boon F, Cain DP. Contribution of sex differences in the acute stress response to sex differences in water maze performance in the rat. Behav Brain Res 2004;151:239–253.

Stackman RW Jr, Lora JC, Williams SB. Directional responding of C57BL/6J mice in the Morris water maze is influenced by visual and vestibular cues and is dependent upon the anterior thalamic nuclei. J Neurosci 2012;32:10211–10225.

Garthe A, Kempermann G. An old test for new neurons: refining the Morris water maze to study the functional relevance of adult hippocampal neurogenesis. Front Neurosci 2013;7:63.

Darwish HA, Arab HH, Abdelsalam RM. Chrysin alleviates testicular dysfunction in adjuvant arthritic rats via suppression of inflammation and apoptosis: comparison with celecoxib. Toxicol Appl Pharmacol 2014;279:129–140.

Csiszar A, Gautam T, Sosnowska D, Tarantini S, Banki E, Tucsek Z, et al. Caloric restriction confers persistent antioxidative, pro-angiogenic and anti-inflammatory effects and promotes anti-aging miRNA expression profile in cerebromicrovascular endothelial cells of aged rats. Am J Physiol Heart Circ Physiol 2014;307:292–306.

Hu HY, Li KP, Wang XJ, Liu Y, Lu ZG, Dong RH, et al. Set9, NF-κB, and microRNA-21 mediate berberineinduced apoptosis of human multiple myeloma cells. Acta Pharmacol Sin 2013;34:157–166.

Wei WT, Chen H, Ni ZL, Liu HB, Tong HF, Fan L, et al. Antitumor and apoptosis-promoting properties of emodin, an anthraquinone derivative from Rheum officinale Baill, against pancreatic cancer in mice via inhibition of Akt activation. Int J Oncol 2011;39:1381–1390.

Jang JY, Kim HY, Kim YR, Choi YW, Choi YH, Lee JH, et al. Hexane extract from Polygonum multiflrum attenuates glutamate-induced apoptosis in primary cultured cortical neurons. J Ethnopharmacol 2013;145:261–268.

Zhang JK, Yang L, Meng GL, Fan J, Chen JZ, He QZ, et al. Protective effect of tetrahydroxystilbene glucoside against hydrogen peroxide induced dysfunction and oxidative stress in osteoblastic MC3T3-E1 cells. Eur J Pharmacol 2012;689:31–37.

Sun YX. Structure and biological activities of the polysaccharides from the leaves, roots and fruits of Panax ginseng C.A. Meyer: an overview. Carbohydr Polym 2011;85:490–499.

Saton K, Fukumoto Y, Shimokawa H. Rho-kinase: important new the acute toxicity of the Rhizoma coptidis extract in mice by increasing the systemic exposure to Rhizoma Coptidis alkal oids. J Ethnopharmacol 2011;138:169.

Carroll JC, Iba M, Bangasser DA, Valentino RJ, James MJ, Brunden KR, et al. Chronic stress exacerbates tau pathology, neurodegeneration and cognitive performance through a corticotropin-releasing factor receptor-dependent mechanism in a transgenic mouse model of tauopathy. J Neurosci 2011;31:14436–14449.

Colom LV, Castaneda MT, Hernandez S, Perry G, Jaime S, Touhami A. Intrahippocampal amyloid-β(1-40) injections injure medial septal neurons in rats. Curr Alzheimer Res 2011;8:832–840.

Marcello E, Epis R, Saraceno C, Di Luca M. Synaptic dysfunction in Alzheimer's disease. Adv Exp Med Biol 2012;970:573–601.

Barnhart CD, Yang DR, Lein PJ. Using the Morris water maze to assess spatial learning and memory in weanling mice. PLoS One 2015;10:e0124521.

Vorhees CV, Williams MT. Assessing spatial learning and memory in rodents. ILAR J 2014;55:310–332.

Schulz K, Korz V. Emotional and cognitive information processing: relations to behavioral performance and hippocampal long-term potentiation in vivo during a spatial water maze training in rats. Learn Mem 2010;17:552–560.

Snigdha S, Smith ED, Prieto GA, Cotman CW. Caspase-3 activation as a bifurcation point between plasticity and cell death. Neurosci Bull 2012;28:14–24.

Colitti M. Bcl-2 family of proteins and mammary cellular fate. Anat Histol Embryol 2012;41:237–247.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

Arsenic disulfide induced apoptosis and concurrently promoted erythroid differentiation in cytokine-dependent myelodysplastic syndrome-progressed leukemia cell line F-36p with complex karyotype including monosomy 7

Related Author

No data

Related Institution

Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan

Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰