Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Qi-qi Xin; Bin-rui Yang; He-feng Zhou; Yan Wang; Bo-wen Yi; Wei-hong Cong; Simon Ming-Yuen Lee; Ke-ji Chen

doi:10.1007/s11655-016-2262-2

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Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Updated：2021-08-27

- Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro
- Chinese Journal of Integrative Medicine Vol. 24, Issue 7, Pages: 494-501(2018)
- Affiliations：
  
  1. Clinical Medical College, Beijing University of Chinese Medicine,Beijing,China
  2. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau,Macao SAR,China
  3. Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  4. Department of Pharmaceutical Preparation, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Science and Technology Development Fund of Macao SAR (No. 078/2011/A3 and 134/2014/A3), Research Committee of University of Macau (No. MYRG2015-00182-ICMS-QRCM), and Beijing Municipal Science and Technology Commission (No. Z141100002214011), China
- DOI：10.1007/s11655-016-2262-2
  CLC：
- Published：2018，
  
  Published Online：9 June 2016，
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Xin, Qq., Yang, Br., Zhou, Hf. et al. Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro., Chin. J. Integr. Med. 24, 494–501 (2018). https://doi.org/10.1007/s11655-016-2262-2

Qi-qi Xin, Bin-rui Yang, He-feng Zhou, et al. Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro. [J]. Chinese Journal of Integrative Medicine 24(7):494-501(2018)
Xin, Qq., Yang, Br., Zhou, Hf. et al. Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro., Chin. J. Integr. Med. 24, 494–501 (2018). https://doi.org/10.1007/s11655-016-2262-2 DOI：

Qi-qi Xin, Bin-rui Yang, He-feng Zhou, et al. Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro. [J]. Chinese Journal of Integrative Medicine 24(7):494-501(2018) DOI： 10.1007/s11655-016-2262-2.

摘要

To investigate the pro-angiogenic effects of paeoniflorin (PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells (HUVECs). In vivo

the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1:EGFP)y1 transgenic zebrafish. The 24 h post fertilization (hpf) embryos were pretreated with vascular endothelial growth factor (VEGF) receptor tyrosine kinase inhibitor II (VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 h. The formation of intersegmental vessels (ISVs) was observed with a fluorescence microscope. The mRNA expression of fms-like tyrosine kinase-1 (flt-1)

kinase insert domain receptor (kdr)

kinase insert domain receptor like (kdrl) and von Willebrand factor (vWF) were analyzed by real-time polymerase chain reaction (PCR). In vitro

the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation

migration and tube formation were assessed. PF (6.25–100 μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF (25–100 μmol/L)

thereby restoring the mRNA expressions of flt-1

kdr

kdrl and vWF

which were down-regulated by VRI treatment. In addition

PF (0.001–0.03 μmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0–10 μmol/L and tube formation at 0.3 μmol/L. PF could promote angiogenesis in a vascular insufficiency model of zebrafish in vivo and in HUVECs in vitro.

Abstract

To investigate the pro-angiogenic effects of paeoniflorin (PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells (HUVECs). In vivo

kinase insert domain receptor (kdr)

kinase insert domain receptor like (kdrl) and von Willebrand factor (vWF) were analyzed by real-time polymerase chain reaction (PCR). In vitro

the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation

migration and tube formation were assessed. PF (6.25–100 μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF (25–100 μmol/L)

thereby restoring the mRNA expressions of flt-1

kdr

kdrl and vWF

which were down-regulated by VRI treatment. In addition

关键词

angiogenesispaeoniflorinzebrafishHuman Umbilical Vein Endothelial Cell

Keywords

angiogenesispaeoniflorinzebrafishHuman Umbilical Vein Endothelial Cell

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Laboratory of Cardiovascular Diseases, Xiyuan Hospital China Heart Institute of Chinese Medicine, China Academy of Chinese Medical Sciences

School of Chinese Medicine, The Chinese University of Hong Kong

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao

Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou

Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University

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