Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1

Jing-si Zhang; Yi-zheng Wang; Ya-qiong Hu; Fan Lin; Dong Gao; Jun Song; Ted J. Kaptchuk; Ke-ji Chen

doi:10.1007/s11655-016-2470-9

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Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1

Updated：2021-08-27

- Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1
- Chinese Journal of Integrative Medicine Vol. 22, Issue 8, Pages: 605-610(2016)
- Affiliations：
  
  1. Zhongshan Hospital, Fudan University,Shanghai,China
  2. College of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Experimental Research Center, China Academy of Chinese Medical Sciences,Beijing,China
  4. Beth Israel Deaconess Medical Center, Harvard Medical School,Boston,USA
  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072933 and No. 81173431);and National Center for Complementary and Alternative Medicine/ National Institutes of Health of USA (No. 2K24 AT004095)
- DOI：10.1007/s11655-016-2470-9
  CLC：
- Published：2016，
  
  Published Online：14 June 2016，
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Zhang, Js., Wang, Yz., Hu, Yq. et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1., Chin. J. Integr. Med. 22, 605–610 (2016). https://doi.org/10.1007/s11655-016-2470-9

Jing-si Zhang, Yi-zheng Wang, Ya-qiong Hu, et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1. [J]. Chinese Journal of Integrative Medicine 22(8):605-610(2016)
Zhang, Js., Wang, Yz., Hu, Yq. et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1., Chin. J. Integr. Med. 22, 605–610 (2016). https://doi.org/10.1007/s11655-016-2470-9 DOI：

Jing-si Zhang, Yi-zheng Wang, Ya-qiong Hu, et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1. [J]. Chinese Journal of Integrative Medicine 22(8):605-610(2016) DOI： 10.1007/s11655-016-2470-9.

摘要

To evaluate the effect of Xuefu Zhuyu Capsule (血府逐瘀胶囊)-containing serum (XFZY-CS) on EphB4/ephrinB2 and its reverse signal in human microvascular endothelial cell-1 (HMEC-1). XFZY-CS and the blank control serum were collected. HMEC-1 cells were randomly assigned to 6 groups including the concentration 1.25%

2.5%

and 5% XFZY-CS groups and their blank serum control ones. The angiogenesis effect of XFZY-CS was tested with an in vitro tube formation assay and the best condition of pro-angiogenesis was determined. The effect of XFZY-CS on EphB4/ephrinB2 and the reverse signal were determined by Western blot and real-time quantitative polymerase chain reaction

respectively; we also confifirmed the results through activating and inhibiting the reverse signal by EphB4/fc and pyrophosphatase/ phosphodiesterase2 (PP2). XFZY-CS promoted angiogenesis at the concentration of 2.5% corresponding serum after being cultured for 48 h

while inhibited angiogenesis at the concentration of 5% after culturing for 48 and 72 h. Under the 2.5% serum concentration

XFZY up-regulated the expression of EphB4-mRNA at 12 h (P<0.05)

and down-regulates its expression at 24 h (P<0.01). Protein expression of EphB4 was apparently up-regulated at 12 h and down-regulated at 24 h. The phosphorylation of ephrinB2 increased at 9 h (P<0.05). In addition

2.5% XFZY-CS played a similar role as the reverse signaling activator EphB4/Fc ranging from 0.5 to 5 μg/mL (P>0.05). XFZY-CS also reduced the inhibitive effect of PP2 in limited periods. EphB4/ephrinB2 was the upstream signal in the process of angiogenesis and its reverse signaling was responsible for XFZY’s effect on promoting angiogenesis.

Abstract

2.5%

while inhibited angiogenesis at the concentration of 5% after culturing for 48 and 72 h. Under the 2.5% serum concentration

XFZY up-regulated the expression of EphB4-mRNA at 12 h (P<0.05)

关键词

Xuefu Zhuyu CapsuleangiogenesisephrinB2/EphB4 reverse signal

Keywords

Xuefu Zhuyu CapsuleangiogenesisephrinB2/EphB4 reverse signal

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