FOLLOWUS
Effects of traditional herbal formulae on human CYP450 isozymes
- Chinese Journal of Integrative Medicine Vol. 23, Issue 1, Pages: 62-69(2017)
- Affiliations：
Herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea,K
- Author bio：
- Funds：Supported by the Construction of Scientific Evidences for Herbal Medicine Formulae (No. K16251) and Evaluation of Herb-Drug Interactions (No. K16252) from the Korea Institute of Oriental Medicine
DOI：10.1007/s11655-016-2476-3
CLC：Published：2017，
Published Online：28 June 2016，
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Jin, S.E., Ha, H. & Shin, HK. Effects of traditional herbal formulae on human CYP450 isozymes., Chin. J. Integr. Med. 23, 62–69 (2017). https://doi.org/10.1007/s11655-016-2476-3
Seong Eun Jin, Hyekyung Ha, Hyeun-Kyoo Shin. Effects of traditional herbal formulae on human CYP450 isozymes. [J]. Chinese Journal of Integrative Medicine 23(1):62-69(2017)
Jin, S.E., Ha, H. & Shin, HK. Effects of traditional herbal formulae on human CYP450 isozymes., Chin. J. Integr. Med. 23, 62–69 (2017). https://doi.org/10.1007/s11655-016-2476-3 DOI：
Seong Eun Jin, Hyekyung Ha, Hyeun-Kyoo Shin. Effects of traditional herbal formulae on human CYP450 isozymes. [J]. Chinese Journal of Integrative Medicine 23(1):62-69(2017) DOI： 10.1007/s11655-016-2476-3.
摘要
To assess the effects of traditional herbal formulae Sijunzi Decoction (四君子汤
Sagunja-tang
SJZD)
Siwu Decoction (四物汤
Samul-tang
SWD)
Bawu Decoction (八物汤
Palmul-tang
BWD) and Shiquan Dabu Decoction (十全大补汤
Sipjeondaebo-tang
SDD) on the activities of human cytochrome P450 (CYP450)
a drug-metabolizing enzyme. Herbal formula water extracts were filtered and lyophilized after the powder extracts were dissolved in distilled water. The activities of major human CYP450 isozymes (CYP3A4
CYP2C19
CYP2D6 and CYP2E1) were measured using in vitro fluorescence-based enzyme assays. The inhibitory effects of the herbal formulas on the activities of CYP450 were characterized as half maximal inhibition concentration (IC50) values. All the tested herbal formulae inhibited CYP2C19 activity (IC50: SJZD
83.28 μg/mL; SWD
235.54 μg/mL; BWD
166.82 μg/mL; SDD
178.19 μg/mL); SJZD (IC50 = 196.46 μg/mL)
SWD (IC50 = 333.42 μg/mL) and SDD (IC50 = 163.42 μg/mL) inhibited CYP2E1-mediated metabolism; whereas BWD exhibited comparatively weak inhibition of CYP2E1 (IC50 = 501.78 μg/mL). None of the four herbal formulas significantly affected CYP3A4 or CYP2D6. These results suggest that SJZD
SWD
BWD and SDD could potentially inhibit the metabolism of co-administered synthetic drugs whose primary route of elimination is via CYP2C19. In addition
clinically relevant pharmacokinetic interactions could occur when SJZD
SWD or SDD is co-administered with drugs metabolized by CYP2E1. Our findings provide information for the safety and effective clinical use of these four classic herbal formulas.
Abstract
To assess the effects of traditional herbal formulae Sijunzi Decoction (四君子汤
Sagunja-tang
SJZD)
Siwu Decoction (四物汤
Samul-tang
SWD)
Bawu Decoction (八物汤
Palmul-tang
BWD) and Shiquan Dabu Decoction (十全大补汤
Sipjeondaebo-tang
SDD) on the activities of human cytochrome P450 (CYP450)
a drug-metabolizing enzyme. Herbal formula water extracts were filtered and lyophilized after the powder extracts were dissolved in distilled water. The activities of major human CYP450 isozymes (CYP3A4
CYP2C19
CYP2D6 and CYP2E1) were measured using in vitro fluorescence-based enzyme assays. The inhibitory effects of the herbal formulas on the activities of CYP450 were characterized as half maximal inhibition concentration (IC50) values. All the tested herbal formulae inhibited CYP2C19 activity (IC50: SJZD
83.28 μg/mL; SWD
235.54 μg/mL; BWD
166.82 μg/mL; SDD
178.19 μg/mL); SJZD (IC50 = 196.46 μg/mL)
SWD (IC50 = 333.42 μg/mL) and SDD (IC50 = 163.42 μg/mL) inhibited CYP2E1-mediated metabolism; whereas BWD exhibited comparatively weak inhibition of CYP2E1 (IC50 = 501.78 μg/mL). None of the four herbal formulas significantly affected CYP3A4 or CYP2D6. These results suggest that SJZD
SWD
BWD and SDD could potentially inhibit the metabolism of co-administered synthetic drugs whose primary route of elimination is via CYP2C19. In addition
clinically relevant pharmacokinetic interactions could occur when SJZD
SWD or SDD is co-administered with drugs metabolized by CYP2E1. Our findings provide information for the safety and effective clinical use of these four classic herbal formulas.
关键词
cytochrome P450Sijunzi DecoctionSiwu decoctionBawu DecoctionShiquan Dabu Decoctionherb-drug interactions
Keywords
cytochrome P450Sijunzi DecoctionSiwu decoctionBawu DecoctionShiquan Dabu Decoctionherb-drug interactions
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