Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells

Yu-yan Zhang; Hui-fen Zhou; Jie-hong Yang; Yu He; Xiao-qiang Chen; Nishinari Katsuyoshi; Hao-fang Wan; Hai-tong Wan

doi:10.1007/s11655-016-2498-x

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Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells

Updated：2021-08-27

- Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells
- Chinese Journal of Integrative Medicine Vol. 22, Issue 4, Pages: 276-283(2016)
- Affiliations：
  
  1. Research Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
  2. Pharmaceutical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
  3. School of Food and Pharmaceutical Engineering, Hubei University of Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81173647, 81473587, 81274176), Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents, and Key Discipline of Zhejiang Traditional Chinese Medicine (Integrated Traditional and Western Medicine, No. 2012-XK-A06)
- DOI：10.1007/s11655-016-2498-x
  CLC：
- Published：2016，
  
  Published Online：9 April 2016，
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Zhang, Yy., Zhou, Hf., Yang, Jh. et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells., Chin. J. Integr. Med. 22, 276–283 (2016). https://doi.org/10.1007/s11655-016-2498-x

Yu-yan Zhang, Hui-fen Zhou, Jie-hong Yang, et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells. [J]. Chinese Journal of Integrative Medicine 22(4):276-283(2016)
Zhang, Yy., Zhou, Hf., Yang, Jh. et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells., Chin. J. Integr. Med. 22, 276–283 (2016). https://doi.org/10.1007/s11655-016-2498-x DOI：

Yu-yan Zhang, Hui-fen Zhou, Jie-hong Yang, et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells. [J]. Chinese Journal of Integrative Medicine 22(4):276-283(2016) DOI： 10.1007/s11655-016-2498-x.

摘要

To observe the effects of Danhong Injection (丹红注射液) and its main components

including daiclzein and hydroxysafflor yellow A (HSYA)

on the anticoagulation

fibrinolysis

anti-apoptosis in hypoxia model of vein endothelial cells (VECs). VECs were prepared and were put in a hypoxia environment

which consisted of mixed gas of 95% N and 5% CO mixed gas

when reached confluent culture. Five groups used different treatments

including normal control group

hypoxia group

daiclzein group

HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection

daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Compared with the normal control group

the floating cells number

PAI activity

ET and MDA contents

and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased

whereas the 6-keto-PGF1α and NO contents

and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group

Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity

regulated PAI and t-PA activities

ET and NO contents

and decreased MDA content and cells apoptosis rate (P<0.05 or P<0.01). Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.

Abstract

To observe the effects of Danhong Injection (丹红注射液) and its main components

including daiclzein and hydroxysafflor yellow A (HSYA)

on the anticoagulation

fibrinolysis

anti-apoptosis in hypoxia model of vein endothelial cells (VECs). VECs were prepared and were put in a hypoxia environment

which consisted of mixed gas of 95% N and 5% CO mixed gas

when reached confluent culture. Five groups used different treatments

including normal control group

hypoxia group

daiclzein group

HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection

the floating cells number

PAI activity

ET and MDA contents

and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased

whereas the 6-keto-PGF1α and NO contents

and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group

Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity

regulated PAI and t-PA activities

ET and NO contents

关键词

vein endothelial cellsDanhong Injectiondaiclzeinhydroxysafflor yellow AanticoagulationfibrinolysisChinese Medicine

Keywords

vein endothelial cellsDanhong Injectiondaiclzeinhydroxysafflor yellow AanticoagulationfibrinolysisChinese Medicine

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