Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest

Wei Gu; Qian Zhang; Chun-sheng Li

doi:10.1007/s11655-016-2505-2

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Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest

Updated：2021-08-27

- Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest
- Chinese Journal of Integrative Medicine Vol. 22, Issue 9, Pages: 666-673(2016)
- Affiliations：
  
  1. Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University,Beijing,China
  2. Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81372025), Basic and Clinical Research Cooperation Project of Capital Medical University (No. 15JL42), and Beijing Municipal Administration of Hospitals Incubating Program (No. Px2016022)
- DOI：10.1007/s11655-016-2505-2
  CLC：
- Published：2016，
  
  Published Online：11 September 2016，
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Gu, W., Zhang, Q. & Li, Cs. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest., Chin. J. Integr. Med. 22, 666–673 (2016). https://doi.org/10.1007/s11655-016-2505-2

Wei Gu, Qian Zhang, Chun-sheng Li. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest. [J]. Chinese Journal of Integrative Medicine 22(9):666-673(2016)
Gu, W., Zhang, Q. & Li, Cs. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest., Chin. J. Integr. Med. 22, 666–673 (2016). https://doi.org/10.1007/s11655-016-2505-2 DOI：

Wei Gu, Qian Zhang, Chun-sheng Li. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest. [J]. Chinese Journal of Integrative Medicine 22(9):666-673(2016) DOI： 10.1007/s11655-016-2505-2.

摘要

To investigate whether Shen-Fu Injection (参附注射液

SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T lymphocytes (Treg) in the spleen. After 8-min untreated ventricular fibrillation and 2-min basic life support

24 pigs were divided into 3 groups with a random number table

i.e. SFI group

epinephrine (EP) group

and saline (SA) group (8 in each group)

which received central venous injection of SFI (1.0 mL/kg)

EP (0.02 mg/kg) and SA

respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC)

apoptosis rate of splenic Treg was detected by flow cytometry; and the mRNA expression of forkhead/winged helix transcription factor (Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay (ELISA). Compared with the sham group

the apoptosis rate of Treg was significantly decreased

and the levels of Foxp3 mRNA expression

IFN-γ

IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups

SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression

IFN-γ and IFN-γ/IL-4 (P<0.05). SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.

Abstract

To investigate whether Shen-Fu Injection (参附注射液

24 pigs were divided into 3 groups with a random number table

i.e. SFI group

epinephrine (EP) group

and saline (SA) group (8 in each group)

which received central venous injection of SFI (1.0 mL/kg)

EP (0.02 mg/kg) and SA

respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC)

the apoptosis rate of Treg was significantly decreased

and the levels of Foxp3 mRNA expression

IFN-γ

IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups

SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression

IFN-γ and IFN-γ/IL-4 (P<0.05). SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.

关键词

Shen-fu Injectioncardiopulmonary resuscitationpost-resuscitation immune dysfunctionregulatory T lymphocytes apoptosisforkhead/winged helix transcription factorChinese Medicine

Keywords

Shen-fu Injectioncardiopulmonary resuscitationpost-resuscitation immune dysfunctionregulatory T lymphocytes apoptosisforkhead/winged helix transcription factorChinese Medicine

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Georg-August-Universität Göttingen, Göttingen

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