Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression

Jing-han Feng; Bao-chang Cai; Wei-feng Guo; Ming-yan Wang; Yong Ma; Qiao-xi Lu

doi:10.1007/s11655-016-2507-0

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Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression

Updated：2021-08-27

- Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression
- Chinese Journal of Integrative Medicine Vol. 23, Issue 2, Pages: 132-137(2017)
- Affiliations：
  
  1. The First Clinical Medical College, Nanjing University of Chinese Medicine,Nanjing,China
  2. Pharmacy College, Nanjing University of Chinese Medicine,Nanjing,China
  3. Basic Medical College, Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2507-0
  CLC：
- Published：2017，
  
  Published Online：9 July 2016，
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Feng, Jh., Cai, Bc., Guo, Wf. et al. Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression., Chin. J. Integr. Med. 23, 132–137 (2017). https://doi.org/10.1007/s11655-016-2507-0

Jing-han Feng, Bao-chang Cai, Wei-feng Guo, et al. Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression. [J]. Chinese Journal of Integrative Medicine 23(2):132-137(2017)
Feng, Jh., Cai, Bc., Guo, Wf. et al. Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression., Chin. J. Integr. Med. 23, 132–137 (2017). https://doi.org/10.1007/s11655-016-2507-0 DOI：

Jing-han Feng, Bao-chang Cai, Wei-feng Guo, et al. Neuroprotective effects of Tongmai Yizhi Decoction (通脉益智汤) against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression. [J]. Chinese Journal of Integrative Medicine 23(2):132-137(2017) DOI： 10.1007/s11655-016-2507-0.

摘要

To explore the protective effects of Tongmai Yizhi Decoction (通脉益智汤

TYD)

a Chinese herb complex prescription against the impairment of cognitive functions and memory loss in amyloid beta 1–40 (Aβ1-40) peptide and ibotenic (IBO)-induced Alzheimer's disease (AD) model rats. The in vivo model was established by injecting Aβ1-40 and IBO into left hippocampal CA1 area of Sprague-Dawley (SD) rat to mimic AD. Totally 32 SD rats were divided into 4 groups

including sham operation group

AD model group

TYD group [AD rats treated with TYD at the dosage of 19.44 g/(kg•d) for 4 weeks] and huperzine A group [AD rats treated with huperzine A at the dosage of 40.5 μg/(kg•d) for 4 weeks]. Spatial learning and memory level was detected by Morris Water Maze test. Histological morphology in the hippocampus was tested by hematoxylin-eosin (HE) staining. Cyclin-dependent kinase-5 (Cdk5) protein and gene expression level were investigated by Western blot analysis and real-time quantitative polymerase chain reaction (RT-qPCR)

respectively. Aβ1-40 and IBO treatment induced longer escape latency of rats

compared with sham operation group from day 25 (P<0.01). However

TYD and huperzine A obviously shortened the escape latency from day 26 (P<0.01). Moreover

the effect of TYD was similar to huperzine A (P>0.05). Furthermore

HE staining also showed that TYD and huperzine A reversed the neuropathological changes in the hippocampus triggered by Aβ1-40 and IBO. TYD and huperzine A effectively reduced the expression levels of Cdk5 protein and gene located in rat hippocampus

compared with the AD model group (P<0.01). TYD could be a promising neuroprotective agent for protecting neuron from AD injury through inhibiting Cdk5 expression.

Abstract

To explore the protective effects of Tongmai Yizhi Decoction (通脉益智汤

TYD)

including sham operation group

AD model group

respectively. Aβ1-40 and IBO treatment induced longer escape latency of rats

compared with sham operation group from day 25 (P<0.01). However

TYD and huperzine A obviously shortened the escape latency from day 26 (P<0.01). Moreover

the effect of TYD was similar to huperzine A (P>0.05). Furthermore

compared with the AD model group (P<0.01). TYD could be a promising neuroprotective agent for protecting neuron from AD injury through inhibiting Cdk5 expression.

关键词

Alzheimer's diseasecyclin-dependent kinase-5Tongmai Yizhi DecoctionChinese Medicine

Keywords

Alzheimer's diseasecyclin-dependent kinase-5Tongmai Yizhi DecoctionChinese Medicine

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Georg-August-Universität Göttingen, Göttingen

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