Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats

Chun-hung Yang; Heng-yuan Chang; Yi-chuan Chen; Chia-chen Lu; Shyh-shyun Huang; Guan-jhong Huang; Hsin-chih Lai

doi:10.1007/s11655-016-2513-2

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Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats

Updated：2021-08-27

- Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats
- Chinese Journal of Integrative Medicine Vol. 23, Issue 2, Pages: 117-124(2017)
- Affiliations：
  
  1. Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, Republic of China
  2. Applied Toxicology Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Executive Yuan, Taichung,Taiwan,China
  3. School of Post-Baccalaureate Chinese Medicine, Tzu Chi University, Hualien,Taiwan,China
  4. Department of Respiratory Therapy, Fu Jen Catholic University, Sinhuang, Taipei,Taiwan,China
  5. School of Pharmacy, College of Pharmacy, China Medical University, Taichung,Taiwan,China
  6. School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung,Taiwan,China
- Author bio：
- Funds：
  
  Supported by China Medical University Tsuzuki Institute for Traditional Medicine (No. CMU95-PH-11 and No. CMU97-232), the National Science Council (No. NSC 95-2320-B-182-059-MY3) and Chang Gung University (No. CMRPD160271), Taiwan, China
- DOI：10.1007/s11655-016-2513-2
  CLC：
- Published：2017，
  
  Published Online：2 August 2016，
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Yang, Ch., Chang, Hy., Chen, Yc. et al. Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats., Chin. J. Integr. Med. 23, 117–124 (2017). https://doi.org/10.1007/s11655-016-2513-2

Chun-hung Yang, Heng-yuan Chang, Yi-chuan Chen, et al. Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats. [J]. Chinese Journal of Integrative Medicine 23(2):117-124(2017)
Yang, Ch., Chang, Hy., Chen, Yc. et al. Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats., Chin. J. Integr. Med. 23, 117–124 (2017). https://doi.org/10.1007/s11655-016-2513-2 DOI：

Chun-hung Yang, Heng-yuan Chang, Yi-chuan Chen, et al. Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats. [J]. Chinese Journal of Integrative Medicine 23(2):117-124(2017) DOI： 10.1007/s11655-016-2513-2.

摘要

To study whether the ethanol extract of Phellinus merrillii (EPM) has chemopreventive potential against liver carcinogenesis. Thirty male Spraque-Dawley rats were randomly divided into control group

EPM control group

hepatocarcinoma control group

low-dose EPM group and high-dose EPM group

6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis

the chemopreventive effect of EPM on diethylnitrosamine (DEN)-initiated

2-acetylaminofluorene (2-AAF) and partial hepatectomy (PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations

together with the serum levels of glutamic oxaloacetic transaminase (sGOT)

glutamic pyruvic transaminase (sGPT) and gamma-glutamyl transpeptidase (γ-GT) were measured. Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of sGOT

sGPT

and serum γ-GT of rats as compared with the hepatocarcinoma control group (P<0.05 or P<0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity. EPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.

Abstract

To study whether the ethanol extract of Phellinus merrillii (EPM) has chemopreventive potential against liver carcinogenesis. Thirty male Spraque-Dawley rats were randomly divided into control group

EPM control group

hepatocarcinoma control group

low-dose EPM group and high-dose EPM group

6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis

the chemopreventive effect of EPM on diethylnitrosamine (DEN)-initiated

2-acetylaminofluorene (2-AAF) and partial hepatectomy (PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations

together with the serum levels of glutamic oxaloacetic transaminase (sGOT)

sGPT

关键词

hepatocarcinogenesisPhellinus merrilliiDiethylnitrosamine2-acetylaminofluorenechemoprevention

Keywords

hepatocarcinogenesisPhellinus merrilliiDiethylnitrosamine2-acetylaminofluorenechemoprevention

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