Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta

Ze-jun Huo; Dong Li; Jia Guo; Sai Li; Ning Ding; Zhi-xin Li

doi:10.1007/s11655-016-2522-1

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Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta

Updated：2021-08-27

- Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta
- Chinese Journal of Integrative Medicine Vol. 22, Issue 10, Pages: 778-782(2016)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, the Third Hospital of Peking University,Beijing,China
  2. Department of Integration of Chinese and Western Medicine, Peking University Health Science Center,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2522-1
  CLC：
- Published：2016，
  
  Published Online：13 July 2016，
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Huo, Zj., Li, D., Guo, J. et al. Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta., Chin. J. Integr. Med. 22, 778–782 (2016). https://doi.org/10.1007/s11655-016-2522-1

Ze-jun Huo, Dong Li, Jia Guo, et al. Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta. [J]. Chinese Journal of Integrative Medicine 22(10):778-782(2016)
Huo, Zj., Li, D., Guo, J. et al. Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta., Chin. J. Integr. Med. 22, 778–782 (2016). https://doi.org/10.1007/s11655-016-2522-1 DOI：

Ze-jun Huo, Dong Li, Jia Guo, et al. Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta. [J]. Chinese Journal of Integrative Medicine 22(10):778-782(2016) DOI： 10.1007/s11655-016-2522-1.

摘要

To observe the effect of electroacupuncture (EA) stimulation on the expressions of angiotensinogen (AGT)

angiotensin II type 1 receptor (AT1R)

endothelin-1 (ET1)

and endothelin A receptor (ETAR) mRNA in spontaneously hypertensive rat (SHR) aorta. Eighteen male SHRs were randomly divided into three groups

an SHR group

an SHR Baihui (DU 20) and Zusanli (ST 36) acupoint (SHR-AP) group

and an SHR non-acupoint (SHR-NAP) group

with 6 rats in each group. Six Wistar rats were used as a control. Rats in the SHR-AP group were stimulated by DU 20 and ST 36 acupoints

both of which were connected with EA. EA was handled one time every Monday

Wednesday and Friday

for total 24 times (8 weeks). SHRNAP rats were acupointed at a 15°angle flat into 0.5 cm to two points

which were 1 and 2 cm from rail tip separately. EA parameters were the same as the SHR-AP rats. SHR control rats and Wistar rats were fixed without EA. Real-time quantitative polymerase chain reaction (PCR) was used to measure AGT

AT1R

ET1

and ETAR mRNA expression in rat aorta. EA stimulation significantly reduced rat aorta vascular AGT

ET1

ETAR and AT1R mRNA expressions in the SHR-AP and SHR-NAP groups (P <0.01). Among these four genes

AT1R mRNA expression was significantly lower in the SHR-AP than in the SHR-NAP group (P <0.01). EA could reduce the AT1R mRNA expression in SHR-AP rat aorta

indicating a potential mechanism for the hypotensive effects of EA.

Abstract

To observe the effect of electroacupuncture (EA) stimulation on the expressions of angiotensinogen (AGT)

angiotensin II type 1 receptor (AT1R)

endothelin-1 (ET1)

and endothelin A receptor (ETAR) mRNA in spontaneously hypertensive rat (SHR) aorta. Eighteen male SHRs were randomly divided into three groups

an SHR group

an SHR Baihui (DU 20) and Zusanli (ST 36) acupoint (SHR-AP) group

and an SHR non-acupoint (SHR-NAP) group

with 6 rats in each group. Six Wistar rats were used as a control. Rats in the SHR-AP group were stimulated by DU 20 and ST 36 acupoints

both of which were connected with EA. EA was handled one time every Monday

Wednesday and Friday

for total 24 times (8 weeks). SHRNAP rats were acupointed at a 15°angle flat into 0.5 cm to two points

AT1R

ET1

and ETAR mRNA expression in rat aorta. EA stimulation significantly reduced rat aorta vascular AGT

ET1

ETAR and AT1R mRNA expressions in the SHR-AP and SHR-NAP groups (P <0.01). Among these four genes

AT1R mRNA expression was significantly lower in the SHR-AP than in the SHR-NAP group (P <0.01). EA could reduce the AT1R mRNA expression in SHR-AP rat aorta

indicating a potential mechanism for the hypotensive effects of EA.

关键词

electricacupuntureangiotensinogenangiotensin II type 1 receptorendothelin-1endothelin-1 A receptor

Keywords

electricacupuntureangiotensinogenangiotensin II type 1 receptorendothelin-1endothelin-1 A receptor

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