Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity

Ji-li Yuan; Yan-yan Tao; Qing-lan Wang; Li Shen; Cheng-hai Liu

doi:10.1007/s11655-016-2540-z

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Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity

Updated：2021-08-27

- Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity
- Chinese Journal of Integrative Medicine Vol. 23, Issue 8, Pages: 598-604(2017)
- Affiliations：
  
  1. Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Shanghai Key Laboratory of Traditional, Chinese Clinical Medicine,Shanghai,China
  3. Institute of TCM Internal Medicine, Shanghai Municipal Education Commission, Shanghai,E,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81270053);the National Science and Technology Major Project (No. 2014ZX10005001) and the International S&T Cooperation Program of China (No. 2014DFA31440);"Three-Year Action Plan" for Development of TCM in Shanghai (No. ZY3-CCCX-2-1003).
- DOI：10.1007/s11655-016-2540-z
  CLC：
- Published：2017，
  
  Published Online：15 February 2017，
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Yuan, Jl., Tao, Yy., Wang, Ql. et al. Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity., Chin. J. Integr. Med. 23, 598–604 (2017). https://doi.org/10.1007/s11655-016-2540-z

Ji-li Yuan, Yan-yan Tao, Qing-lan Wang, et al. Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity. [J]. Chinese Journal of Integrative Medicine 23(8):598-604(2017)
Yuan, Jl., Tao, Yy., Wang, Ql. et al. Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity., Chin. J. Integr. Med. 23, 598–604 (2017). https://doi.org/10.1007/s11655-016-2540-z DOI：

Ji-li Yuan, Yan-yan Tao, Qing-lan Wang, et al. Fuzheng Huayu Formula (扶正化瘀方) prevents rat renal interstitial fibrosis induced by HgCl2 via antioxidative stress and down-regulation of nuclear factor-kappa B activity. [J]. Chinese Journal of Integrative Medicine 23(8):598-604(2017) DOI： 10.1007/s11655-016-2540-z.

摘要

To investigate the mechanism of action of Fuzheng Huayu Formula (扶正化瘀方

FZHY) against renal interstitial fibrosis (RIF) relating to oxidative injury and nuclear factor-kappa B (NF-κB) activity. Thirty-two Sprague-Dawley rats were randomly divided into 3 groups: normal group

model group and FZHY treatment group. The RIF model was induced by oral administration of HgCl2 at a dose of 8 mg/kg body weight once a day for 9 weeks. Meanwhile

rats in FZHY treatment group orally took FZHY at a dose of 4.0 g/kg rat weight for 9 weeks. The content of hydroxyproline (Hyp) and collagen deposition in kidney were observed. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)

the content of glutathione (GSH) and malondialdehyde (MDA) of kidney were tested. The expressions of inhibitor-κappa B (IκB)

phospho-IκB (p-IκB)

tumor necrosis factor-α (TNF-α)

matrix metalloproteinase-2 (MMP-2) and α-smooth muscle actin (α-SMA) were analyzed by Western blot. α-SMA expression was also observed by immunofluorescent staining. MMP-2 activity was measured by gelatin zymography. NF-κB activation was determined by electrophoretic mobility shift assay. Renal interstitial fibrosis was induced by HgCl2

demonstrated by remarkably increased Hyp contents and excessive collagen deposition in kidney (P<0.01). FZHY significantly inhibited renal interstitial collagen deposition and reduced Hyp content of the HgCl2-treated rats (P<0.01). GSH content decreased obviously

and MDA content increased signifificantly in HgCl2-treated rats compared with that of normal rats (P<0.01). FZHY significantly increased GSH content and decreased MDA content in the model rats (P<0.01). The expression α-SMA was increased in model rats compared with that of normal rats

FZHY signifificantly decreased its expression (P<0.01). The expressions of p-IκB and TNF-α and MMP-2

MMP-2 activity

and NF-κB activation were increased in model group compared with that in normal group (P<0.01)

FZHY signifificantly decreased NF-κB activation

MMP-2 activity and p-IκB and TNF-α expressions (P<0.01). FZHY could protect kidney from oxidative injury intoxicated by HgCl2

and antagonized oxidative stress-stimulated NF-κB activity through inhibition of IκB phosphorylation in the interstitial fibrotic kidney

these effects importantly contributed to FZHY action mechanism against renal interstitial fifibrosis.

Abstract

To investigate the mechanism of action of Fuzheng Huayu Formula (扶正化瘀方

model group and FZHY treatment group. The RIF model was induced by oral administration of HgCl2 at a dose of 8 mg/kg body weight once a day for 9 weeks. Meanwhile

the content of glutathione (GSH) and malondialdehyde (MDA) of kidney were tested. The expressions of inhibitor-κappa B (IκB)

phospho-IκB (p-IκB)

tumor necrosis factor-α (TNF-α)

FZHY signifificantly decreased its expression (P<0.01). The expressions of p-IκB and TNF-α and MMP-2

MMP-2 activity

and NF-κB activation were increased in model group compared with that in normal group (P<0.01)

FZHY signifificantly decreased NF-κB activation

MMP-2 activity and p-IκB and TNF-α expressions (P<0.01). FZHY could protect kidney from oxidative injury intoxicated by HgCl2

and antagonized oxidative stress-stimulated NF-κB activity through inhibition of IκB phosphorylation in the interstitial fibrotic kidney

these effects importantly contributed to FZHY action mechanism against renal interstitial fifibrosis.

关键词

Fuzheng Huayu Formularenal interstitial fifibrosismercuric chlorideoxidative stressnuclear factor-kappa Bmatrix metalloproteinase-2tumor necrosis factor-αChinese Medicine

Keywords

Fuzheng Huayu Formularenal interstitial fifibrosismercuric chlorideoxidative stressnuclear factor-kappa Bmatrix metalloproteinase-2tumor necrosis factor-αChinese Medicine

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