Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration

Li-na Liang; Yu-yang Bai; You-zhi Tang; Qiang Chen; Xue-li Li; Qun-ying Ma; Jie Liang; Jiao Li

doi:10.1007/s11655-016-2584-0

Your Location：

Home >

Browse articles >

Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration

Updated：2021-08-27

- Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration
- Chinese Journal of Integrative Medicine Vol. 24, Issue 6, Pages: 442-447(2018)
- Affiliations：
  
  Ophthalmology Research Laboratory, Eye Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2584-0
  CLC：
- Published：2018，
  
  Published Online：12 May 2016，
Scan for full text
Liang, Ln., Bai, Yy., Tang, Yz. et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration., Chin. J. Integr. Med. 24, 442–447 (2018). https://doi.org/10.1007/s11655-016-2584-0

Li-na Liang, Yu-yang Bai, You-zhi Tang, et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration. [J]. Chinese Journal of Integrative Medicine 24(6):442-447(2018)
Liang, Ln., Bai, Yy., Tang, Yz. et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration., Chin. J. Integr. Med. 24, 442–447 (2018). https://doi.org/10.1007/s11655-016-2584-0 DOI：

Li-na Liang, Yu-yang Bai, You-zhi Tang, et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration. [J]. Chinese Journal of Integrative Medicine 24(6):442-447(2018) DOI： 10.1007/s11655-016-2584-0.

摘要

To observe the contribution of Borneolum syntheticum to the intervention effect of Liuwei Dihuang Pill (六味地黄丸

LDP) on experimental retinal degeneration

and initially investigate the mechanism of Borneolum syntheticum as meridian-lead-in drug. A total of 180 sodium iodateinduced retinital degeneration rats were randomly divided into three groups

including distilled water group

LDP group

and LDP+Borneolum syntheticum (LDP+BS) group. Twenty normal rats were fed regularly without any treatment as normal control. On day 7 and 14 after treatment

histopathological study and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) test were performed to evaluate the retinopathy. Claudin-5 expression at blood-retina barrier (BRB) was detected by Western blot at different time points from 0.5 to 8 h after gavage. On day 7 and 14 after treatment

the retinal lesion grades were significantly different among the three groups (P<0.05). The grade in the LDP+BS group was significantly less than the LDP and distilled water groups (both P<0.05)

no significant difference was observed between the LDP and distilled water groups (P>0.05). The apoptosis rates in the LDP+BS group was significantly less than the distilled water and LDP groups (both P<0.05)

while there was no significant difference between LDP and distilled water groups (P>0.05). Expression of claudin-5 in LDP+BS group was significantly less than the other two groups at 0.5

1 and 2 h after gavage (P<0.05). There was no apparent difference among the three groups at 4 and 8 h after gavage (P>0.05). Borneolum syntheticum could strengthen the effect of LDP on experimental retinal degeneration

indicated that Borneolum syntheticum might play the role of meridian-lead-in drug in the formula. The mechanism may be due to Borneolum syntheticum could promote the physiologically openness of bloodretina barrier through transiently affecting the expression of claudin-5.

Abstract

To observe the contribution of Borneolum syntheticum to the intervention effect of Liuwei Dihuang Pill (六味地黄丸

LDP) on experimental retinal degeneration

and initially investigate the mechanism of Borneolum syntheticum as meridian-lead-in drug. A total of 180 sodium iodateinduced retinital degeneration rats were randomly divided into three groups

including distilled water group

LDP group

and LDP+Borneolum syntheticum (LDP+BS) group. Twenty normal rats were fed regularly without any treatment as normal control. On day 7 and 14 after treatment

the retinal lesion grades were significantly different among the three groups (P<0.05). The grade in the LDP+BS group was significantly less than the LDP and distilled water groups (both P<0.05)

while there was no significant difference between LDP and distilled water groups (P>0.05). Expression of claudin-5 in LDP+BS group was significantly less than the other two groups at 0.5

关键词

meridian-lead-in drugBorneolum syntheticumLiuwei Dihuang PillRetinal Degeneration

Keywords

meridian-lead-in drugBorneolum syntheticumLiuwei Dihuang PillRetinal Degeneration

references

Jiang ZH, Zhang J. Discuss on local differentiation and systemic differentiation in TCM ophthalmology. China Pract Med (Chin) 2008;3:91–92.

Jiang P, Fu P, Xiang L, Wang S, Liu X, Yang L, et al. The effectiveness of borneol on pharmacokinetics changes of four ginsenosides in Shexiang Baoxin Pill in vivo. Biomed Chromatogr 2014;28:419–427.

Yang HB, Li ZJ, Yu NN, Jin D, Zhao XM, Cui H. Effect of borneol in improving the permeability of blood-ocular barrier. Int J Ophthalmol (Chin) 2008;8:1576–1578.

Enzmann V, Row BW, Yamauchi Y, Kheirandish L, Gozal D, Kaplan HJ, et al. Behavioral and anatomieal abnormalities in a sodium iodate-induced model of retinal pigment epithelium degeneration. Exp Eye Res 2006;82:441–448.

Liang LN, Wang ZQ, MA Na, Ma QY, Li J, Tang YZ, et al. Contribution of Radix Saposhnikoviae to the intervention effect of Liuwei Dihuang Pills on experimental retinal degeneration. China J Chin Ophthalmol 2013;23:313–317.

Zhu YT, Deng XG, Gao Y, He MF, Li N. Pathological changes and activity of SOD and CAT in the damaged retina induced by sodium iodate in rats. Chin J Pathophysiol (Chin) 2010;26:1851–1854.

Sorsby A. Experimental pigmentary degeneration of the retina by sodium iodate. Br J Ophthalmol 1941;25:58–62.

Kannan R, Hinton DR. Sodium iodate induced retinal degeneration: new insights from an old model. Neural Regen Res 2014;9:2044–2045.

Machaliska A, Lejkowska R, Duchnik M, Kawa M, Rogiska D, Wiszniewska B, et al. Dose-dependent retinal changes following sodium iodate administration: application of spectraldomain optical coherence tomography for monitoring of retinal injury and endogenous regeneration. Curr Eye Res 2014;39:1033–1041.

Carido M, Zhu Y, Postel K, Benkner B, Cimalla P, Karl MO, et al. Characterization of a mouse model with complete RPE loss and its use in RPE cell transplantation. Invest Ophthalmol Vis Sci 2014;55:5431–5444.

Franco LM, Zulliger R, Wolf-Schnurrbusch UE, Katagiri Y, Kaplan HJ, Wolf S, et al. Decreased visual function after patchy loss of retinal pigment epithelium induced by low-dose sodium iodate. Invest Ophthalmol Vis Sci 2009;50:4004–4010.

Guan Y, Cui L, Qu Z, Lu L, Wang F, Wu Y, et al. Subretinal transplantation of rat MSCs and erythropoietin gene modified rat MSCs for protecting and rescuing degenerative retina in rats. Curr Mol Med 2013;13:1419–1431.

Kiuchi K, Yoshizawa K, Shikata N, Moriguchi K, Tsubura A. Morphologic characteristics of retinal degeneration induced by sodium iodate in mice. Curr Eye Res 2002;25:373379.

Wang J, Iacovelli J, Spencer C, Saint-Geniez M. Direct effect of sodium iodate on neurosensory retina. Invest Ophthalmol Vis Sci 2014;55:1941–1952.

Zhao RZ, Liu SJ. TCM medicinal guide theory and target-tropism administration. J Tradit Chin Med (Chin) 2005;46:643–645

Liu N, Gao XC. The study of blood-brain barrier opening by Borneol. Acta Acad Med Weifang (Chin) 2007;29:398–400

Ge CL, Han MF, Bai RT, Yu C. Effect of borneol on the ultrastructure of promoting blood brain barrier Open. Chin J Integr Med Cardio-/Cerebrovasc Dis (Chin) 2008;6:1183–1185.

Campbell M, Nguyen AT, Kiang AS, Tam L, Kenna PF, Dhubhghaill SN, et al. Reversible and size-selective opening of the inner blood-retina barrier: a novel therapeutic strategy. Adv Exp Med Biol 2010;664:301–308.

Campbell M, Humphries MM, Humphries P. Barrier modulation in drug delivery to the retina. Methods Mol Biol 2013;935:371–380.

Campbell M, Nguyen ATH, Kiang AS, Tam LC, Gobbo OL, Kersken C, et al. An experimental platform for systemic drug delivery to the retina. Proc Natl Acad Sci 2009;106:17817–17822.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Study on treatment of retinitis pigmentosa with traditional Chinese medicine by flicker electroretinogram

Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression

Related Author

No data

Related Institution

Shanghai First People’s Hospital

China Academy of TCM

Key Research Laboratory of Osteoporosis Syndrome Genomics, Fujian Academy of Traditional Chinese Medicine

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰