Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats

Lin Li; Yan-lin Li; Yun-feng Zhou; Zheng-yan Ge; Li-li Wang; Zhi-qiang Li; Yu-jie Guo; Long Jin; Ye Ren; Jian-xun Liu; Yang Xu

doi:10.1007/s11655-016-2595-x

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Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats

Updated：2021-08-27

- Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats
- Chinese Journal of Integrative Medicine Vol. 23, Issue 6, Pages: 425-432(2017)
- Affiliations：
  
  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China
  2. Experiment Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Science and Technology Major Project of China (No. 2010ZX09102-213), Beijing Natural Science Foundation, China (No. 7152100), and Program for Innovative Research Team in the Institute of Medicinal Plant Development, China (No. IT 1305)
- DOI：10.1007/s11655-016-2595-x
  CLC：
- Published：2017，
  
  Published Online：23 June 2016，
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Li, L., Li, Yl., Zhou, Yf. et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats., Chin. J. Integr. Med. 23, 425–432 (2017). https://doi.org/10.1007/s11655-016-2595-x

Lin Li, Yan-lin Li, Yun-feng Zhou, et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats. [J]. Chinese Journal of Integrative Medicine 23(6):425-432(2017)
Li, L., Li, Yl., Zhou, Yf. et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats., Chin. J. Integr. Med. 23, 425–432 (2017). https://doi.org/10.1007/s11655-016-2595-x DOI：

Lin Li, Yan-lin Li, Yun-feng Zhou, et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats. [J]. Chinese Journal of Integrative Medicine 23(6):425-432(2017) DOI： 10.1007/s11655-016-2595-x.

摘要

To evaluate the prevention effect of diabetic retinopathy of Jiangtang Xiaozhi Recipe (降糖消脂方

JXR) in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were randomly divided into normal control group and diabetic group. Rats in the diabetic group were induced by intraperitoneal administration of STZ (50 mg/kg)

and subdivided into 5 groups. Rats in the diabetic control group were given saline; four treatment groups were given metformin (300 mg/kg)

JXR (2

4 and 8 g/kg) respectively for 8 weeks

while rats in the normal control group were injected with citrate buffer and given the same volume of vehicle. Body weight and food intake were measured every week. The hypoglycaemic effects were determined by testing fasting blood glucose (FBG) every other week

and hemoglobin A1c (HbA1c)

insulin

and glucagon at the end of the treatment. The preventive effects of JXR on STZ-induced diabetic rats were determined by histopathological examination with hematoxylin and eosin staining

and periodic acid-schiff staining. The effects were further evaluated by serum superoxide dismutase (SOD) activity and malondialdehyde (MDA). High-dose JXR significantly reduced FBG and HbA1c level at the 8th week of administration (P<0.01

P<0.05). JXR significantly increased insulin level (P<0.05)

and decreased glucagon level (P<0.05). JXR showed the antioxidant defense with increased SOD activity and decreased MDA contents in diabetic rats. Histopathological studies revealed that there were no basement membrane thickening and mild destruction in the treated groups. Morphometric measurements of retina microvascular showed that acellular capillary and capillary density decreased in treated rats while pericyte and endothelial cell increasing after the treatment. JXR have protective effect of diabetic retinopathy and its mechanism may be associated with the obvious hypoglycemic and antioxidant effect.

Abstract

To evaluate the prevention effect of diabetic retinopathy of Jiangtang Xiaozhi Recipe (降糖消脂方

and subdivided into 5 groups. Rats in the diabetic control group were given saline; four treatment groups were given metformin (300 mg/kg)

JXR (2

4 and 8 g/kg) respectively for 8 weeks

and hemoglobin A1c (HbA1c)

insulin

and glucagon at the end of the treatment. The preventive effects of JXR on STZ-induced diabetic rats were determined by histopathological examination with hematoxylin and eosin staining

P<0.05). JXR significantly increased insulin level (P<0.05)

关键词

Jiangtang Xiaozhi RecipeChinese Medicinestreptozotocindiabetes mellitusdiabetic retinopathy

Keywords

Jiangtang Xiaozhi RecipeChinese Medicinestreptozotocindiabetes mellitusdiabetic retinopathy

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