Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain

Li-li Ji; Meng-wei Guo; Xiu-jun Ren; Dong-yu Ge; Gen-mao Li; Ya Tu

doi:10.1007/s11655-016-2606-y

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Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain

Updated：2021-08-27

- Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain
- Chinese Journal of Integrative Medicine Vol. 23, Issue 10, Pages: 786-792(2017)
- Affiliations：
  
  1. School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine,Beijing,China
  2. School of Basic Medical Science, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Project of Beijing University of Chinese Medicine, China (No. JYB22-JS022)
- DOI：10.1007/s11655-016-2606-y
  CLC：
- Published：2017，
  
  Published Online：24 October 2016，
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Ji, Ll., Guo, Mw., Ren, Xj. et al. Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain., Chin. J. Integr. Med. 23, 786–792 (2017). https://doi.org/10.1007/s11655-016-2606-y

Li-li Ji, Meng-wei Guo, Xiu-jun Ren, et al. Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain. [J]. Chinese Journal of Integrative Medicine 23(10):786-792(2017)
Ji, Ll., Guo, Mw., Ren, Xj. et al. Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain., Chin. J. Integr. Med. 23, 786–792 (2017). https://doi.org/10.1007/s11655-016-2606-y DOI：

Li-li Ji, Meng-wei Guo, Xiu-jun Ren, et al. Effects of electroacupuncture intervention on expression of cyclooxygenase 2 and microglia in spinal cord in rat model of neuropathic pain. [J]. Chinese Journal of Integrative Medicine 23(10):786-792(2017) DOI： 10.1007/s11655-016-2606-y.

摘要

To investigate the effect of electroacupuncture (EA) treatment on the expression of cyclooxygenase (COX) 2 and microglia in spinal cord by using rat model of neuropathic pain

and to probe into the relationship between COX 2 and microglia. The rats were randomly divided into 6 groups

including normal control group

model group

sham group

EA 1 group (distant acupoints + local acupoints)

EA 2 group (local acupoints)

and EA 3 group (distant acupoints). Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3

5 and 7 days postoperatively. At 7 days postoperatively

the spinal COX 2 mRNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Compared with the model group

thermal withdrawal latencies increased after EA treatment (P<0.01). The expressions of COX 2 mRNA were up-regulated in spinal cord of rat on day 7 after surgery (P<0.05). Compared with the model group

EA stimulation (EA 1 and EA 2 groups) reversed the up-regulation of COX 2 mRNA expression (P<0.05). EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 mRNA in the trend of the therapeutic effect of "distant acupoints + local acupoints"

and "local acupoints" intervention may be superior to that of "distant acupoints" intervention. Microglia may be related to the formation of COX 2.

Abstract

To investigate the effect of electroacupuncture (EA) treatment on the expression of cyclooxygenase (COX) 2 and microglia in spinal cord by using rat model of neuropathic pain

and to probe into the relationship between COX 2 and microglia. The rats were randomly divided into 6 groups

including normal control group

model group

sham group

EA 1 group (distant acupoints + local acupoints)

EA 2 group (local acupoints)

and EA 3 group (distant acupoints). Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3

5 and 7 days postoperatively. At 7 days postoperatively

and "local acupoints" intervention may be superior to that of "distant acupoints" intervention. Microglia may be related to the formation of COX 2.

关键词

neuropathic painelectroacupuncturemicrogliacyclooxygenase 2Spinal Cord

Keywords

neuropathic painelectroacupuncturemicrogliacyclooxygenase 2Spinal Cord

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Department of Acupuncture and Moxibustion, Xixi Hospital of Hangzhou

Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of Zhejiang Chinese Medical University

Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University

Department of Anesthesiology, The Second Affiliated Hospital of Wenzhou Medical College

Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital of Wenzhou Medical College

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