Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats

Yang Cheng; Jing-yin Mai; Mei-feng Wang; Gao-feng Chen; Jian Ping

doi:10.1007/s11655-016-2627-6

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Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats

Updated：2021-08-27

- Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats
- Chinese Journal of Integrative Medicine Vol. 23, Issue 1, Pages: 48-54(2017)
- Affiliations：
  
  1. Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Shanghai Pudong New Area Hospital of Traditional Chinese Medicine,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the Shanghai Natural Science Foundation (No.10ZR1430700) and Three-Year Plan of Action of Traditional Chinese Medicine in Shanghai (No. ZY3-RCPY-1-1011)
- DOI：10.1007/s11655-016-2627-6
  CLC：
- Published：2017，
  
  Published Online：27 October 2016，
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Cheng, Y., Mai, Jy., Wang, Mf. et al. Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats., Chin. J. Integr. Med. 23, 48–54 (2017). https://doi.org/10.1007/s11655-016-2627-6

Yang Cheng, Jing-yin Mai, Mei-feng Wang, et al. Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats. [J]. Chinese Journal of Integrative Medicine 23(1):48-54(2017)
Cheng, Y., Mai, Jy., Wang, Mf. et al. Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats., Chin. J. Integr. Med. 23, 48–54 (2017). https://doi.org/10.1007/s11655-016-2627-6 DOI：

Yang Cheng, Jing-yin Mai, Mei-feng Wang, et al. Antifibrotic effect of total flavonoids of Astmgali Radix on dimethylnitrosamine-induced liver cirrhosis in rats. [J]. Chinese Journal of Integrative Medicine 23(1):48-54(2017) DOI： 10.1007/s11655-016-2627-6.

摘要

To study the effect of total flavonoids of Astmgali Radix (TFA) on liver cirrhosis induced with dimethylnitrosamine (DMN) in rats

and the effect on peroxisome proliferator-activated receptor γ (PPARγ)

uncoupling protein 2 (UCP2) and farnesoid X receptor (FXR). Fifty-three Sprague-Dawley rats were randomly divided into a control group (10 rats) and a DMN group (43 rats). Rats in the DMN group were given DMN for 4 weeks and divided randomly into a model group (14 rats)

a low-dosage TFA group (14 rats) and a high-dosage TFA group (15 rats) in the 3rd week. Rats were given TFA for 4 weeks at the dosage of 15 and 30 mg/kg in the low- and high-TFA groups

respectively. At the end of the experiment blood and liver samples were collected. Serum liver function and liver tissue hydroxyproline content were determined. hematoxylin-eosin (HE)

Sirus red and immunohistochemical stainings of collagen I

smooth muscle actin (α-SMA) was conducted in paraffinembedded liver tissue slices. Real time polymerase chain reaction (PCR) was adopted to determine PPARγ

UCP2 and FXR mRNA levels. Western blot was adopted to determine protein levels of collagen I

α-SMA

PPARγ

UCP2 and FXR. Compared with the model group

TFA increased the ratio of liver/body weight (low-TFA group P<0.05

high-TFA group P<0.01)

improved liver biochemical indices (P<0.01 for ALT

AST

GGT in both groups

P<0.05 for albumin and TBil in the high-TFA group) and reduced liver tissue hydroxproline content (P<0.01 in both groups) in treatment groups significantly. HE staining showed that TFA alleviated liver pathological changes markedly and Sirus red staining showed that TFA reduced collagen deposition

alleviated formation and extent of liver pseudolobule. Collagen I and α-SMA immunohistochemical staining showed that staining area and extent markedly decreased in TFA groups compared with the model group. TFA could increase PPARγ

it regulated target UCP2

and FXR levels significantly compared with the model group (in the low-TFA group all P<0.05

in the high group all P<0.01). TFA could improve liver function

alleviate liver pathological changes

and reduce collagen deposition and formation of liver pseudolobule in rats with liver cirrhosis. The antifibrotic effect of TFA was through regulating PPARγ signal pathway and the interaction with FXR.

Abstract

To study the effect of total flavonoids of Astmgali Radix (TFA) on liver cirrhosis induced with dimethylnitrosamine (DMN) in rats

and the effect on peroxisome proliferator-activated receptor γ (PPARγ)

a low-dosage TFA group (14 rats) and a high-dosage TFA group (15 rats) in the 3rd week. Rats were given TFA for 4 weeks at the dosage of 15 and 30 mg/kg in the low- and high-TFA groups

respectively. At the end of the experiment blood and liver samples were collected. Serum liver function and liver tissue hydroxyproline content were determined. hematoxylin-eosin (HE)

Sirus red and immunohistochemical stainings of collagen I

smooth muscle actin (α-SMA) was conducted in paraffinembedded liver tissue slices. Real time polymerase chain reaction (PCR) was adopted to determine PPARγ

UCP2 and FXR mRNA levels. Western blot was adopted to determine protein levels of collagen I

α-SMA

PPARγ

UCP2 and FXR. Compared with the model group

TFA increased the ratio of liver/body weight (low-TFA group P<0.05

high-TFA group P<0.01)

improved liver biochemical indices (P<0.01 for ALT

AST

GGT in both groups

it regulated target UCP2

and FXR levels significantly compared with the model group (in the low-TFA group all P<0.05

in the high group all P<0.01). TFA could improve liver function

alleviate liver pathological changes

关键词

total flavonoids of Astmgali Radixdimethylnitrosamineliver cirrhosisperoxisome proliferatoractivated receptor γuncoupling protein 2farnesoid X receptorrat

Keywords

total flavonoids of Astmgali Radixdimethylnitrosamineliver cirrhosisperoxisome proliferatoractivated receptor γuncoupling protein 2farnesoid X receptorrat

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