Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways

Ke-fei Hu; Xiang-ying Kong; Mi-cun Zhong; Hong-ye Wan; Na Lin; Xiao-hua Pei

doi:10.1007/s11655-016-2647-2

Your Location：

Home >

Browse articles >

Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways

Updated：2021-08-27

- Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways
- Chinese Journal of Integrative Medicine Vol. 23, Issue 2, Pages: 110-116(2017)
- Affiliations：
  
  1. Beijing University of Chinese Medicine,Beijing,China
  2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
  3. Department of Scientific Research and Education, Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Fifty-fifth Batch of China Post Doctoral Science Foundation (No. 2014M550663)
- DOI：10.1007/s11655-016-2647-2
  CLC：
- Published：2017，
  
  Published Online：29 December 2016，
Scan for full text
Hu, Kf., Kong, Xy., Zhong, Mc. et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways., Chin. J. Integr. Med. 23, 110–116 (2017). https://doi.org/10.1007/s11655-016-2647-2

Ke-fei Hu, Xiang-ying Kong, Mi-cun Zhong, et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways. [J]. Chinese Journal of Integrative Medicine 23(2):110-116(2017)
Hu, Kf., Kong, Xy., Zhong, Mc. et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways., Chin. J. Integr. Med. 23, 110–116 (2017). https://doi.org/10.1007/s11655-016-2647-2 DOI：

Ke-fei Hu, Xiang-ying Kong, Mi-cun Zhong, et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways. [J]. Chinese Journal of Integrative Medicine 23(2):110-116(2017) DOI： 10.1007/s11655-016-2647-2.

摘要

To examine the effects of brucine on the invasion

migration and bone resorption of receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis. The osteoclastogenesis model was builded by co-culturing human breast tumor MDA-MB-231 and mouse RAW264.7 macrophages cells. RANKL (50 ng/mL) and macrophage-colony stimulating factor (50 ng/mL) were added to this system

followed by treatment with brucine (0.02

0.04 and 0.08 mmol/L)

or 10 μmol/L zoledronic acid as positive control. The migration and bone resorption were measured by transwell assay and in vitro bone resorption assay. The protein expressions of Jagged1 and Notch1 were investigated by Western blot. The expressions of transforming growth factor-β1 (TGF-β1)

nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Compared with the model group

brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells

inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P<0.01). Furthermore

brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1

NF-κB and Hes1 (P<0.05 or P<0.01). Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways.

Abstract

To examine the effects of brucine on the invasion

followed by treatment with brucine (0.02

0.04 and 0.08 mmol/L)

nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Compared with the model group

brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells

inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P<0.01). Furthermore

brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1

NF-κB and Hes1 (P<0.05 or P<0.01). Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways.

关键词

BrucineBreast CancerBone MetastasisJagged1/Notch1 signaling pathway

Keywords

BrucineBreast CancerBone MetastasisJagged1/Notch1 signaling pathway

references

Zhang Y, Ma B, Fan Q. Mechanisms of breast cancer bone metastasis. Cancer Lett 2010;292:1–7.

Mundy GR. Metastasis to bone: causes, consequences and therapeutic opportunities. Nat Rev Cancer 2002;2:584–593.

Mundy GR. Mechanisms of bone metastasis. Cancer 1997;80:1546–1556.

Clezardin P. Therapeutic targets for bone metastases in breast cancer. Breast Cancer Res 2011;13:207.

Hofbauer LC, Kuhne CA, Viereck V. The OPG/RANKL/RANK system in metabolic bone diseases. J Musculoskelet Neuronal Interact 2004;4:268–275.

Kang Y, Siegel PM, Shu W, Drobnjak M, Kakonen SM, Cordon-Cardo C, et al. A multigenic program mediating breast cancer metastasis to bone. Cancer Cell 2003;3:537–549.

Fukushima H. Regulatory mechanisms of Notch signaling in osteoclast differentiation. J Oral Biosciences 2010;52:205–214.

Chiechi A, Waning DL, Stayrook KR, Buijs JT, Guise TA, Mohammad KS. Role of TGF-ß in breast cancer bone metastases. Adv Biosci Biotechnol 2013;4:15–30.

Park BK, Zhang H, Zeng Q, Dai J, Keller ET, Giordano T, et al. NF-kappaB in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GMCSF. Nat Med 2007;13:62–69.

Kuramoto T, Goto H, Mitsuhashi A, Tabata S, Ogawa H, Uehara H, et al. Dll4-Fc, an inhibitor of Dll4-notch signaling, suppresses liver metastasis of small cell lung cancer cells through the downregulation of the NF-kappaB activity. Mol Cancer Ther 2012;11:2578–2587.

Zhang W, Grivennikov SI. Top Notch cancer stem cells by paracrine NF-kappaB signaling in breast cancer. Breast Cancer Res 2013;15:316.

Sethi N, Dai X, Winter CG, Kang Y. Tumor-derived JAGGED1 promotes osteolytic bone metastasis of breast cancer by engaging notch signaling in bone cells. Cancer Cell 2011;19:192–205.

Sethi N, Kang Y. Notch signalling in cancer progression and bone metastasis. Br J Cancer 2011;105:1805–1810.

Zhang P, Yang Y, Zweidler-McKay PA, Hughes DP. Critical role of notch signaling in osteosarcoma invasion and metastasis. Clin Cancer Res 2008;14:2962–2969.

Zhao LM, Liu YG, Niu ZX. Anti-tumor effect on brucine. Chin J Cancer Treat (Chin) 2013;20:877–880.

Serasanambati M, Chilakapati SR, Manikonda PK, Kanala JR, Chilakapati DR. Anticancer effects of brucine and gemcitabine combination in MCF-7 human breast cancer cells. Nat Prod Res 2015;29:484–490.

Agrawal SS, Saraswati S, Mathur R, Pandey M. Cytotoxic and antitumor effects of brucine on Ehrlich ascites tumor and human cancer cell line. Life Sci 2011;89:147–158.

Deng XK, Yin W, Li WD, Yin FZ, Lu XY, Zhang XC, et al. The anti-tumor effects of alkaloids from the seeds of Strychnos nux-vomica on HepG2 cells and its possible mechanism. J Ethnopharmacol 2006;106:179–186.

Deng X, Yin F, Lu X, Cai B, Yin W. The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway. Toxicol Sci 2006;91:59–69.

Ma WJ, Li P. Effects of brucine on breast cancer bone metastasis in mice. Anhui Med Pharm (Chin) 2009;13:600–602.

Li P, Zhang M, Ma WJ, Sun X, Jin FP. Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer. Chin J Integr Med 2012;18:605–609.

Lowery FJ, Yu D. Growth factor signaling in metastasis: current understanding and future opportunities. Cancer Metastasis Rev 2012;31:479–491.

Schneider D, Liaw L, Daniel C, Athanasopoulos AN, Herrmann M, Preissner KT, et al. Inhibition of breast cancer cell adhesion and bone metastasis by the extracellular adherence protein of Staphylococcus aureus. Biochem Biophys Res Commun 2007;357:282–288.

Dempster DW, Moonga BS, Stein LS, Horbert WR, Antakly T. Glucocorticoids inhibit bone resorption by isolated rat osteoclasts by enhancing apoptosis. J Endocrinol 1997;154:397–406.

Bai S, Kopan R, Zou W, Hilton MJ, Ong CT, Long F, et al. NOTCH1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells. J Biol Chem 2008;283:6509–6518.

Hofbauer LC, Lacey DL, Dunstan CR, Spelsberg TC, Riggs BL, Khosla S. Interleukin-1beta and tumor necrosis factoralpha, but not interleukin-6, stimulate osteoprotegerin ligand gene expression in human osteoblastic cells. Bone 1999;25:255–259.

Cao L, Arany PR, Wang YS, Mooney DJ. Promoting angiogenesis via manipulation of VEGF responsiveness with notch signaling. Biomaterials 2009;30:4085–4093.

Li MO, Wan YY, Sanjabi S, Robertson AK, Flavell RA. Transforming growth factor-beta regulation of immune responses. Annu Rev Immunol 2006;24:99–146.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer

Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions

Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats

Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer

Safely using TCM herbs: Adverse reactions and precautions (continued)

Related Author

No data

Related Institution

Department of Oncology, Affiliated Hospital of Bengbu Medical College

Department of Traditional Chinese Medicine Oncology, Provincial Hospital of Anhui Medical University

Department of Chinese Medicine Tumor, the Affiliated Provincial Hospital of Anhui Medical University

Department of Geriatrics, the First Affiliated Hospital of Bengbu Medical College

Department of Pharmacology, College of Pharmacy, Fujian Medical University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰