Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia

Bin Han; Chun-xia Zhu; Wan Shi; Hui-zhu Huang; Xu-guang Hu; Xiao-ming Zhou; Ming Lei; Zhong Li

doi:10.1007/s11655-016-2649-0

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Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia

Updated：2021-08-27

- Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia
- Chinese Journal of Integrative Medicine Vol. 23, Issue 7, Pages: 535-542(2017)
- Affiliations：
  
  College of Traditional Chinese Medicine, Guangdong Pharmaceutical University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81173194)
- DOI：10.1007/s11655-016-2649-0
  CLC：
- Published：2017，
  
  Published Online：24 January 2017，
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Han, B., Zhu, Cx., Shi, W. et al. Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia., Chin. J. Integr. Med. 23, 535–542 (2017). https://doi.org/10.1007/s11655-016-2649-0

Bin Han, Chun-xia Zhu, Wan Shi, et al. Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia. [J]. Chinese Journal of Integrative Medicine 23(7):535-542(2017)
Han, B., Zhu, Cx., Shi, W. et al. Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia., Chin. J. Integr. Med. 23, 535–542 (2017). https://doi.org/10.1007/s11655-016-2649-0 DOI：

Bin Han, Chun-xia Zhu, Wan Shi, et al. Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia. [J]. Chinese Journal of Integrative Medicine 23(7):535-542(2017) DOI： 10.1007/s11655-016-2649-0.

摘要

To explore the effects of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility (PR) on uric acid metabolism and the expression of urinary neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) in rats with hyperuricemia. Seventy male Sprague Dawley (SD) rats were randomly divided into 7 groups with 10 rats per group

including the normal group

model group

allopurinol group

benzbromarone group and PR groups at 3 doses (3.5

14 g/kg). Except the normal group

rats of the other groups were intragastrically administered 100 mg/kg hypoxanthine and 250 mg/kg ethambutol

and subcutaneously injected with 200 mg/kg potassium oxonate. All rats were continuously modeled for 17 days

and gavaged with corresponding drugs. The rats of the normal and model groups were gavaged with saline

once a day

for 2 weeks. The levels of serum uric acid (SUA)

blood urea nitrogen (BUN) and creatinine (Cr) were determined. In addition

the contents of NGAL and KIM-1 in urine and the mRNA and protein expressions of xanthine oxidase (XOD) in liver of hyperuricemia rats were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot

respectively. Moreover

the pathological changes of kidney were analyzed by hematoxylin and eosin (HE) stain method. Compared with the normal group

the levels of SUA

BUN

NGAL and KIM-1 and the expressions of hepatic XOD mRNA and protein in the hyperuricemia rats were increased signifificantly (P<0.01). PR signifificantly decreased the levels of SUA

BUN

NGAL and KIM-1 and down-regulated the mRNA and protein expressions of hepatic XOD (P<0.05 or P<0.01). In addition

the pathological changes of kidney were signifificantly suppressed by oral administration of PR. PR ameliorated uric acid metabolism and protected renal function

the underlying mechanism was mediated by decreasing the levels of SUA

BUN

NGAL and KIM-1

inhibiting the expression of hepatic XOD and ameliorating the pathological change of kidney.

Abstract

including the normal group

model group

allopurinol group

benzbromarone group and PR groups at 3 doses (3.5

14 g/kg). Except the normal group

rats of the other groups were intragastrically administered 100 mg/kg hypoxanthine and 250 mg/kg ethambutol

and subcutaneously injected with 200 mg/kg potassium oxonate. All rats were continuously modeled for 17 days

and gavaged with corresponding drugs. The rats of the normal and model groups were gavaged with saline

once a day

for 2 weeks. The levels of serum uric acid (SUA)

blood urea nitrogen (BUN) and creatinine (Cr) were determined. In addition

respectively. Moreover

the pathological changes of kidney were analyzed by hematoxylin and eosin (HE) stain method. Compared with the normal group

the levels of SUA

BUN

NGAL and KIM-1 and the expressions of hepatic XOD mRNA and protein in the hyperuricemia rats were increased signifificantly (P<0.01). PR signifificantly decreased the levels of SUA

BUN

NGAL and KIM-1 and down-regulated the mRNA and protein expressions of hepatic XOD (P<0.05 or P<0.01). In addition

the pathological changes of kidney were signifificantly suppressed by oral administration of PR. PR ameliorated uric acid metabolism and protected renal function

the underlying mechanism was mediated by decreasing the levels of SUA

BUN

NGAL and KIM-1

inhibiting the expression of hepatic XOD and ameliorating the pathological change of kidney.

关键词

hyperuricemiaRhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibilityXanthine Oxidaseneutrophil gelatinase-associated lipocalinkidney injury molecule-1Chinese Medicine

Keywords

hyperuricemiaRhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibilityXanthine Oxidaseneutrophil gelatinase-associated lipocalinkidney injury molecule-1Chinese Medicine

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