Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine

Shi-wei Liu; Xiao-yang Xu; Jie Xu; Jiu-yun Yuan; Wei-kang Wu; Ning Zhang; Ze-liang Chen

doi:10.1007/s11655-016-2738-0

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Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine

Updated：2021-08-27

- Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine
- Chinese Journal of Integrative Medicine Vol. 23, Issue 10, Pages: 763-769(2017)
- Affiliations：
  
  1. Department of Endocrinology, Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Institute of Disease Control and Prevention, Academy of Military Medical Sciences,Beijing,China
  3. Department of Clinical Laboratory, The First Affiliated, Hospital of Soochow University,Jiangsu Province,Suzhou,China
  4. Institute of Integrative Medicine, Sun Yat-sen University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Key Program for Infectious Diseases of China (Nos. 2013ZX10004-203), National Natural Science Foundation of China (No. 81401646), Chinese Academy of Traditional Chinese Medicine Joint Innovation Research Project (No. ZZ070808), and Capital Featured Clinical Application and Product Promotion Project (No. Z151100004015132)
- DOI：10.1007/s11655-016-2738-0
  CLC：
- Published：2017，
  
  Published Online：27 December 2016，
Scan for full text
Liu, Sw., Xu, Xy., Xu, J. et al. Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine., Chin. J. Integr. Med. 23, 763–769 (2017). https://doi.org/10.1007/s11655-016-2738-0

Shi-wei Liu, Xiao-yang Xu, Jie Xu, et al. Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine. [J]. Chinese Journal of Integrative Medicine 23(10):763-769(2017)
Liu, Sw., Xu, Xy., Xu, J. et al. Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine., Chin. J. Integr. Med. 23, 763–769 (2017). https://doi.org/10.1007/s11655-016-2738-0 DOI：

Shi-wei Liu, Xiao-yang Xu, Jie Xu, et al. Multi-drug resistant uropathogenic Escherichia coli and its treatment by Chinese medicine. [J]. Chinese Journal of Integrative Medicine 23(10):763-769(2017) DOI： 10.1007/s11655-016-2738-0.

摘要

To investigate the resistance and virulence profiles of uropathogenic Escherichia coli (UPEC) and its treatment by Chinese medicine (CM) Fuzheng Qingre Lishi Formula (扶正清热利湿方

FQLF). UPEC strains were isolated from recurrent urinary tract infections (UTIs) patients. Patient sensitivities to 17 antibiotics were tested by the disk diffusion method. Virulence genes were screened by plolymerase chain reaction. A mouse model was constructed using a multi-drug resistant and virulent UPEC strain and treated with FQLF or the antibiotic imipenem. The treatment efficacy was evaluated by bacterial clearance from urine and the urinary organs. A total of 90 UPEC strains were collected

and 94.4% of the isolates were resistant to at least 1 antibiotic. Approximately 66.7% of the UPEC strains were multi-drug resistant. More than one virulence gene was found in 85.6% of the isolates. The extended-spectrum β-lactamases (ESBL)-positive strains were more resistant than the negative ones. The virulence gene number was positively correlated with the resistance number (P<0.05). A mouse model was successfully constructed using UPEC10. Treatment with either FQLF or antibiotics significantly cleared bacteria from the mouse urine after 14 days. In the untreated control

the bacteria lasted for 28 days. FQLF treatment of the UTI mouse model greatly reduced the bacterial number in the kidney and bladder

but could not completely clear the bacteria. Multi-drug resistance is common among UPEC isolates

and the resistance is positively related with virulence. FQLF could treat UPEC UTIs

but could not completely clear the bacteria from the host.

Abstract

To investigate the resistance and virulence profiles of uropathogenic Escherichia coli (UPEC) and its treatment by Chinese medicine (CM) Fuzheng Qingre Lishi Formula (扶正清热利湿方

the bacteria lasted for 28 days. FQLF treatment of the UTI mouse model greatly reduced the bacterial number in the kidney and bladder

but could not completely clear the bacteria. Multi-drug resistance is common among UPEC isolates

and the resistance is positively related with virulence. FQLF could treat UPEC UTIs

but could not completely clear the bacteria from the host.

关键词

urinary tract infectionuropathogenic Escherichia coliChinese Medicinemulti-drug resistance

Keywords

urinary tract infectionuropathogenic Escherichia coliChinese Medicinemulti-drug resistance

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