Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression

Ji-rong Ge; Li-hua Xie; Juan Chen; Sheng-qiang Li; Hui-juan Xu; Yu-lian Lai; Long-long Qiu; Chen-bo Ni

doi:10.1007/s11655-016-2744-2

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Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression

Updated：2021-08-27

- Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression
- Chinese Journal of Integrative Medicine Vol. 24, Issue 6, Pages: 415-422(2018)
- Affiliations：
  
  Key Research Laboratory of Osteoporosis Syndrome Genomics, Fujian Academy of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2744-2
  CLC：
- Published：2018，
  
  Published Online：27 December 2016，
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Ge, Jr., Xie, Lh., Chen, J. et al. Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression., Chin. J. Integr. Med. 24, 415–422 (2018). https://doi.org/10.1007/s11655-016-2744-2

Ji-rong Ge, Li-hua Xie, Juan Chen, et al. Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression. [J]. Chinese Journal of Integrative Medicine 24(6):415-422(2018)
Ge, Jr., Xie, Lh., Chen, J. et al. Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression., Chin. J. Integr. Med. 24, 415–422 (2018). https://doi.org/10.1007/s11655-016-2744-2 DOI：

Ji-rong Ge, Li-hua Xie, Juan Chen, et al. Liuwei Dihuang Pill (六味地黄丸) Treats Postmenopausal Osteoporosis with Shen (Kidney) Yin Deficiency via Janus Kinase/Signal Transducer and Activator of Transcription Signal Pathway by Up-regulating Cardiotrophin-Like Cytokine Factor 1 Expression. [J]. Chinese Journal of Integrative Medicine 24(6):415-422(2018) DOI： 10.1007/s11655-016-2744-2.

摘要

To investigate the mechanism of Liuwei Dihuang Pill (六味地黄丸

LDP) in treating postmenopausal osteoporosis (PMOP) with Shen (Kidney) yin deficiency. In this study

205 cases of PMOP were divided into the PMOP Shen-yin deficiency group (Group A)

PMOP Shen-yang deficiency group (Group B)

PMOP without Shen deficiency group (Group C)

and control group (Group N). Real-time polymerase chain reaction (RT-PCR) and Western blot techniques were used to observe the effects of LDP treatment on the cardiotrophin-like cytokine factor 1 (CLCF1)

ankyrin repeat and SOCS box containing 1 (ASB1)

and prokineticin 2 (PROK2) genes and the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. The mRNA (P<0.05) and protein (P<0.01) expression levels of the CLCF1 gene in Group A were significantly lower than the corresponding levels in Group N. After LDP treatment for 3 months

the mRNA expression levels of the CLCF1 gene were obviously up-regulated (P<0.01). After 6-month treatment

the expression levels of CLCF1 mRNA and protein were significantly up-regulated (both P<0.01)

and the average bone density of the top femur had significantly increased (P<0.05). In vitro

CLCF1 overexpression resulted in a significant increase in the total protein and phosphorylated protein levels of JAK2 and STAT3. The CLCF1 gene is an important gene associated with PMOP Shen-yin deficiency and the therapeutic effects of LDP may be mediated by up-regulation of CLCF1 gene expression and activation of the JAK/STAT signaling pathway.

Abstract

To investigate the mechanism of Liuwei Dihuang Pill (六味地黄丸

LDP) in treating postmenopausal osteoporosis (PMOP) with Shen (Kidney) yin deficiency. In this study

205 cases of PMOP were divided into the PMOP Shen-yin deficiency group (Group A)

PMOP Shen-yang deficiency group (Group B)

PMOP without Shen deficiency group (Group C)

ankyrin repeat and SOCS box containing 1 (ASB1)

the mRNA expression levels of the CLCF1 gene were obviously up-regulated (P<0.01). After 6-month treatment

the expression levels of CLCF1 mRNA and protein were significantly up-regulated (both P<0.01)

and the average bone density of the top femur had significantly increased (P<0.05). In vitro

关键词

postmenopausal osteoporosisChinese MedicineShen (Kidney) yin deficiencycardiotrophinlike cytokine factor 1 geneLiuwei Dihuang PillJanus kinase/signal transducer and activator of transcription signaling pathway

Keywords

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