Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome

Yi Wen; Yan Wang; Ting-ting Feng; Shao-bin Wei

doi:10.1007/s11655-017-2401-4

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Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome

Updated：2021-08-27

- Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome
- Chinese Journal of Integrative Medicine Vol. 24, Issue 12, Pages: 925-929(2018)
- Affiliations：
  
  Gynecology Department of Traditional Chinese Medicine, Clinical Medical College, Chengdu University of Traditional Chinese Medicine,Chengdu,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30973770)
- DOI：10.1007/s11655-017-2401-4
  CLC：
- Published：2018，
  
  Published Online：24 January 2017，
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Wen, Y., Wang, Y., Feng, Tt. et al. Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome., Chin. J. Integr. Med. 24, 925–929 (2018). https://doi.org/10.1007/s11655-017-2401-4

Yi Wen, Yan Wang, Ting-ting Feng, et al. Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome. [J]. Chinese Journal of Integrative Medicine 24(12):925-929(2018)
Wen, Y., Wang, Y., Feng, Tt. et al. Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome., Chin. J. Integr. Med. 24, 925–929 (2018). https://doi.org/10.1007/s11655-017-2401-4 DOI：

Yi Wen, Yan Wang, Ting-ting Feng, et al. Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome. [J]. Chinese Journal of Integrative Medicine 24(12):925-929(2018) DOI： 10.1007/s11655-017-2401-4.

摘要

To investigate the innate characters of 3 endometriosis (EMT) syndromes

blood stasis (BS)

qi stagnation and blood stasis (QSBS) as well as Shen (Kidney) deficiency and blood stasis (KDBS) in terms of proteomics

lay a molecular biological basis for the differentiation of various blood stasis syndromes of EMT

establish a EMT microscopic syndrome differentiation and diagnosis system in terms of proteomics

discover the evolution principles and therapeutic targets of these EMT syndromes

and search their signifificant molecular markers and genetic intervention targets. Six specimens from the ectopic and entopic endometrium tissues of patients with EMT in each syndrome

QSBS as well as KDBS

in the early proliferative phase of the menstrual cycle

and 6 specimens from normal endometrium tissues in the early proliferative phase of the menstrual cycle were obtained. Three groups were formed in each syndrome by mixing two random specimens in equal amount

and then their respective two-dimensional electrophoresis graphs were obtained after total protein extraction. Finally

the detected differences in protein expression were identifified through matrix-assisted laser desorption Ionization-time of flflight mass spectrometry (MALDI-TOF/MS) and protein database. The results of differential proteins expressed in each syndrome were shown as follows: BS syndrome had 2 differential proteins in entopic endometrium and 1 differential protein in ectopic endometrium; KDBS syndrome had 3 in entopic endometrium and 3 in ectopic endometrium; and QSBS syndrome had 3 in entopic endometrium and 4 in ectopic endometrium. It was found out that annexin was highly expressed in both entopic and ectopic endometrium of KDBS syndrome; and myosin light chain 3 was highly expressed in both entopic and ectopic endometrium of QSBS syndrome. There are differential protein expressions among the 3 EMT syndromes

which might be the inner origin of syndrome characters

and these differential proteins might be the candidate biomarkers for the pathogenesis of various EMT syndromes.

Abstract

To investigate the innate characters of 3 endometriosis (EMT) syndromes

blood stasis (BS)

qi stagnation and blood stasis (QSBS) as well as Shen (Kidney) deficiency and blood stasis (KDBS) in terms of proteomics

lay a molecular biological basis for the differentiation of various blood stasis syndromes of EMT

establish a EMT microscopic syndrome differentiation and diagnosis system in terms of proteomics

discover the evolution principles and therapeutic targets of these EMT syndromes

and search their signifificant molecular markers and genetic intervention targets. Six specimens from the ectopic and entopic endometrium tissues of patients with EMT in each syndrome

QSBS as well as KDBS

in the early proliferative phase of the menstrual cycle

and then their respective two-dimensional electrophoresis graphs were obtained after total protein extraction. Finally

which might be the inner origin of syndrome characters

and these differential proteins might be the candidate biomarkers for the pathogenesis of various EMT syndromes.

关键词

endometriosisBlood Stasis Syndromeproteomicstwo-dimensional electrophoresismass spectrum

Keywords

endometriosisBlood Stasis Syndromeproteomicstwo-dimensional electrophoresismass spectrum

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