FOLLOWUS
Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels
- Chinese Journal of Integrative Medicine Vol. 23, Issue 9, Pages: 654-662(2017)
- Affiliations：
1. Clinical Medical College, Beijing University of Chinese Medicine,Beijing,China
2. Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
3. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau,Macao SAR,China
4. Department of Acupuncture and Moxibustion, Qilu Hospital Affiliated to Shandong University,Jinan,China
- Author bio：
- Funds：Supported by Beijing Municipal Science and Technology Commission (No. Z141100002214011), the Science and Technology Development Fund of Macao SAR (No. 069/2015/A2 and 134/2014/A3) and Research Committee of University of Macau (No. MYRG2015-00182-ICMS-QRCM, MYRG2015-00214-ICMS-QRCM, MYRG139(Y1-L4)-ICMS12-LMY, and MYRG2016-00129-ICMS-QRCM), China;Conflict of Interests;The authors claimed no potential conflicts of interest relevant to this article.;Author Contributions;Wang Y and Guo G contributed equally to this work. Chen KJ, Lee SM, Cong WH, Yang BR, Xin QQ and Wang Y conceived and designed the experiments;Yang BR, HU YJ, Liao QW performed the network pharmacology analysis;Wang Y, Guo G, Xin QQ performed the zebrafish experiments. Wang Y and Guo G contributed significantly to analysis and manuscript preparation. Chen KJ, Lee SM and Cong WH helped perform the analysis with constructive discussions.;Electronic Supplementary Materials;: Supplementary materials (Appendixes 1-2) are available in the online version of this article at http://dx.doi.org/10.1007/s11655-017-2408-x
DOI：10.1007/s11655-017-2408-x
CLC：Published：2017，
Published Online：27 May 2017，
Scan for full text
Wang, Y., Guo, G., Yang, Br. et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels., Chin. J. Integr. Med. 23, 654–662 (2017). https://doi.org/10.1007/s11655-017-2408-x
Yan Wang, Gang Guo, Bin-rui Yang, et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels. [J]. Chinese Journal of Integrative Medicine 23(9):654-662(2017)
Wang, Y., Guo, G., Yang, Br. et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels., Chin. J. Integr. Med. 23, 654–662 (2017). https://doi.org/10.1007/s11655-017-2408-x DOI：
Yan Wang, Gang Guo, Bin-rui Yang, et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels. [J]. Chinese Journal of Integrative Medicine 23(9):654-662(2017) DOI： 10.1007/s11655-017-2408-x.
摘要
To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis in silico and in vivo. The mechanisms of action of an herb-pair
Chuanxiong-Chishao
were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis
and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks
including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore
the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-induced vascular insuffificiency model of transgenic zebrafifish in vivo. The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain reaction (RT-PCR). The computational prediction results found that the compounds in Chuanxiong have antithrombotic
antihypertensive
antiarrhythmic
and antiatherosclerotic activities
which were closely related to protecting against hypoxic-ischemic encephalopathy
ischemic stroke
myocardial infarction and heart failure. In addition
compounds in Chishao were found to participate in anti-inflflammatory effect and analgesics. Particularly
estrogen receptor α (ESRα) and hypoxia-inducible factor 1-α (HIF-1α) were the most important potential protein targets in the predicted results. In vivo experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood vessels in zebrafifish involving up-regulating ESRα mRNA expression. Co-treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insuffificiency zebrafifish at the optimal compatibility proportion of PF 10 μmol/L with TMP•HCl 1 μmol/L. The network pharmacological strategies combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover
the transgenic zebrafifish experiments demonstrated that the Chuanxiong-Chishao combination synergistically promoted angiogenic activity
probably involving ESRα signaling pathway.
Abstract
To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis in silico and in vivo. The mechanisms of action of an herb-pair
Chuanxiong-Chishao
were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis
and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks
including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore
the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-induced vascular insuffificiency model of transgenic zebrafifish in vivo. The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain reaction (RT-PCR). The computational prediction results found that the compounds in Chuanxiong have antithrombotic
antihypertensive
antiarrhythmic
and antiatherosclerotic activities
which were closely related to protecting against hypoxic-ischemic encephalopathy
ischemic stroke
myocardial infarction and heart failure. In addition
compounds in Chishao were found to participate in anti-inflflammatory effect and analgesics. Particularly
estrogen receptor α (ESRα) and hypoxia-inducible factor 1-α (HIF-1α) were the most important potential protein targets in the predicted results. In vivo experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood vessels in zebrafifish involving up-regulating ESRα mRNA expression. Co-treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insuffificiency zebrafifish at the optimal compatibility proportion of PF 10 μmol/L with TMP•HCl 1 μmol/L. The network pharmacological strategies combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover
the transgenic zebrafifish experiments demonstrated that the Chuanxiong-Chishao combination synergistically promoted angiogenic activity
probably involving ESRα signaling pathway.
关键词
tetramethylpyrazinepaeoniflorinangiogenesisnetwork pharmacology
Keywords
tetramethylpyrazinepaeoniflorinangiogenesisnetwork pharmacology
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