Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice

Gi Hee Jeong; William A. Boisvert; Mei-zhu Xi; Yi-lin Zhang; Young-Bin Choi; Sunghun Cho; Sanghyun Lee; Changsun Choi; Bog-Hieu Lee

doi:10.1007/s11655-017-2755-7

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Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice

Updated：2021-08-27

- Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice
- Chinese Journal of Integrative Medicine Vol. 24, Issue 8, Pages: 591-599(2018)
- Affiliations：
  
  1. Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Republic of Korea
  2. Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawaii,Honolulu,USA
  3. Department of Integrative Plant Science, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Republic of Korea
- Author bio：
- Funds：
  
  Supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration (No. C0142798) in 2013;and by the Chung-Ang University Research Grants in 2015
- DOI：10.1007/s11655-017-2755-7
  CLC：
- Published：2018，
  
  Published Online：11 May 2017，
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Jeong, G.H., Boisvert, W.A., Xi, Mz. et al. Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice., Chin. J. Integr. Med. 24, 591–599 (2018). https://doi.org/10.1007/s11655-017-2755-7

Gi Hee Jeong, William A. Boisvert, Mei-zhu Xi, et al. Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice. [J]. Chinese Journal of Integrative Medicine 24(8):591-599(2018)
Jeong, G.H., Boisvert, W.A., Xi, Mz. et al. Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice., Chin. J. Integr. Med. 24, 591–599 (2018). https://doi.org/10.1007/s11655-017-2755-7 DOI：

Gi Hee Jeong, William A. Boisvert, Mei-zhu Xi, et al. Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice. [J]. Chinese Journal of Integrative Medicine 24(8):591-599(2018) DOI： 10.1007/s11655-017-2755-7.

摘要

To investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models. MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92–62.50 μg/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 μg/mL. The expression of transforming growth factor β1 (TGF-β1)

hepatocyte growth factor (HGF)

β-catenin

substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed. MSP (7.81 μg/mL) down-regulated TGF-β1 and up-regulated HGF and β-catenin in hDPCs (P<0.01). MSP (1000 μg/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01). The MSP flower extract may have hair growth-promotion activities.

Abstract

hepatocyte growth factor (HGF)

β-catenin

关键词

Miscanthus sinensis var. purpurascens flower extracthair growthhuman hair dermal papilla cellhepatocyte growth factortransforming growth factor β1

Keywords

Miscanthus sinensis var. purpurascens flower extracthair growthhuman hair dermal papilla cellhepatocyte growth factortransforming growth factor β1

references

Botchkarev VA, Kishimoto J. Molecular control of epithelialmesenchymal interactions during hair follicle cycling. J Investig Dermatol Symp Proc 2003;8:46–55.

Huelsken J, Vogel R, Erdmann B, Cotsarelis G, Birchmeier W. β-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell 2001;105:533–545.

Danilenko DM, Ring BD, Pierce GF. Growth factors and cytokines in hair follicle development and cycling: recent insights from animal models and the potentials for clinical therapy. Mol Med Today 1996;2:460–467.

Arck PC, Handjiski B, Hagen E, Joachim R, Klapp BF, Paus R. Indications for a ‘brain-hair follicle axis (BHA)’: inhibition of keratinocyte proliferation and up-regulation of keratinocyte apoptosis in telogen hair follicles by stress and substance P. FASEB J 2001;15:2536–2538.

Paus R. Stress, hair growth control and the neuroendocrine immune connection. Allergo J 2000;9:411–420.

Ebner K, Singewald N. The role of substance P in stress and anxiety responses. Amino Acids 2006;31:251–272.

Suzuki H, Miura S, Liu YY, Tsuchiya M, Ishii H. Substance P induces degranulation of mast cells and leukocyte adhesion to venular endothelium. Peptides 1995;16:1447–1452.

Lee GY, Kim GS, and Cheong CH. Miscanthus sinensis var. purpurascens (Andersson) Rendle. In: Lee GY, Kim GS, Cheong CH, eds. Medicinal herbs growing in Korea. Korea Forest Seed and Variety Center, Chungbuk, Republic of Korea: Seoul Marketing Company;2013:496–497.

Lee JA. Identification and standardization of bioactive compounds from roots of Miscanthus sinensis var. purpurascens and Peucedanum japonicum Thunberg [dissertation]. Jeju: Jeju National University;2011. (KNS ver.)

Kim EJ, Choi JY, Park BC, Lee BH. Platycarya strobilacea S.et Z.extract has a high antioxidant capacity and exhibits hair growth-promoting effects in male C57BL/6 mice. Prev Nutr Food Sci 2014;19:136–144.

Palumbo ML, Canzobre MC, Pascuan CG, Ríos H, Wald M, Genaro AM. Stress induced cognitive deficit is differentially modulated in BALB/c and C57Bl/6 mice: correlation with Th1/Th2 balance after stress exposure. J Neuroimmunol 2010;218:12–20.

Kong MK, Kim YC. Promotion effect of black sesame oil on hair growth in an alopecia model of C57BL/6 mice. J Biomed Res 2010;11:103–116.

Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. J Food Sci Technol 2012;44:337–342.

Naito A, Midorikawa T, Yoshino T, Ohdera M. Lipid peroxides induce early onset of catagen phase in murine hair cycles. Int J Mol Med 2008;22:725–729.

Hah DS, Kim CH, Kim GS, Kim ES, Kim JS. Antioxidative effects of traditional medicinal plants on lipid peroxidation. Korean J Vet Res 2005;45:341–350.

Seger R, Krebs EG. The MAPK signaling cascade. FASEB J 1995;9:726–735.

Jindo T, Tsuboi R, Imai R, Takamori K, Rubin JS, Ogawa H. The effect of hepatocyte growth factor/scatter factor on human hair follicle growth. J Dermatol Sci 1995;10:229–232.

Yamauchi K, Kurosaka A. Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture. Arch Dermatol Res 2009;301:357–365.

Kumamoto T, Shalhevet D, Matsue H, Mummert ME, Ward BR, Jester JV, et al. Hair follicles serve as local reservoirs of skin mast cell precursors. Blood 2003;102:1654–1660.

Arck PC, Handjiski B, Peters EM, Hagen E, Klapp BF, Paus R. Topical minoxidil counteracts stress-induced hair growth inhibition in mice. Exp Dermatol 2003;12:580–590.

Peters EM, Maurer M, Botchkarev VA, Jensen Kd, Welker P, Scott GA, et al. Kit is expressed by epithelial cells In vivo. J Invest Dermatol 2003;121:976–984.

Tsuji Y, Denda S, Soma T, Raftery L, Momoi T, Hibino T. A potential suppressor of TGF-β delays catagen progression in hair follicles. J Investig Dermatol Symp Proc 2003;8:65–68.

Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest 2001;107:409–417.

Lim EJ, Kang HJ, Jung HJ, Kim J, Lim CJ, Park EH. Antiinflammatory, anti-angiogenic and anti-nociceptive activities of 4-hydroxybenzaldehyde. Biomol Ther 2008;16:231–236.

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