Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 <italic style="font-style: italic">in vitro</italic> and <italic style="font-style: italic">in vivo</italic> Using <italic style="font-style: italic">Salvia miltiorrhiza</italic> Bunge.

Woong Jin Bae; Jin Bong Choi; Kang Sup Kim; U Syn Ha; Sung Hoo Hong; Ji Youl Lee; Tae-Kon Hwang; Sung Yeoun Hwang; Zhi-ping WANG; Sae Woong Kim

doi:10.1007/s11655-017-2801-5

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Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge.

Original Article | Updated：2021-08-23

- Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge.
- Chinese Journal of Integrative Medicine Vol. 26, Issue 7, Pages: 533-538(2020)
- Affiliations：
  
  1.Department of Urology, The Catholic University of Korea, College of Medicine, Seoul (06591), Republic of Korea
  2.Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul (06591), Republic of Korea
  3.Korea Bio-Medical Science Institute, Seoul (06106), Republic of Korea
  4.Department of Urology, Second Hospital of Lanzhou University, Lanzhou (730030), China
- Author bio：
  
  Correspondence to: Dr. Sae Woong Kim, E-mail:ksw1227@catholic.ac.kr
- Funds：
- DOI：10.1007/s11655-017-2801-5
  CLC：
- Published：01 July 2020，
  
  Published Online：23 March 2017，
  
  Accepted：03 May 2012
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Woong Jin Bae, Jin Bong Choi, Kang Sup Kim, et al. Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge.. [J]. Chinese Journal of Integrative Medicine 26(7):533-538(2020)
DOI：

Woong Jin Bae, Jin Bong Choi, Kang Sup Kim, et al. Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge.. [J]. Chinese Journal of Integrative Medicine 26(7):533-538(2020) DOI： 10.1007/s11655-017-2801-5.

摘要

Abstract

Objective:

To investigate the antiproliferative activity of

Salvia miltiorrhiza

Bunge. (SM) on the castration-resistant prostate cancer (CRPC) cell line DU-145

in vitro

and

in vivo

Methods:

Prostate cancer cell line (DU-145) and normal prostate cell line (RWPE-1) were treated with SM at different concentrations (3.125

12.5

25 and 50 μg/mL) to investigate the antiproliferative effects. DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells. Molecular mechanism was investigated by Western blot analysis of p53

Bcl-2

prostate specific antigen (PSA)

and androgen receptor (AR). Six-week-old male BALB/c nude mice were randomly divided into normal control group (

=101) and treated group (

=101) which administered 500 mg/kg SM for 2 weeks. Tumor volumes were measured.

Results:

Treatment with SM resulted in a dose-dependent decrease in cell number of DU-145 cells in comparison with RWPE-1. DNA laddering analysis indicated the apoptosis of DU-145 cells. Treatment with SM increased the expression of p53 and reduced the expression of Bcl-2 proteins. The levels of PSA were considerably reduced in SM-treated group compared to the controls

and a decrease in AR expression was observed when cells were treated with SM in the same pattern as a reduction in PSA. In the tumour xenograft study

SM given once a day for 2 weeks significantly inhibited tumour growth.

Conclusion:

SM might contribute to the anticancer actions such as induction of apoptosis and inhibition of proliferation of prostate cancer cells.

关键词

Keywords

apoptosisprostate cancerSalvia miltiorrhiza Bunge.Chinese medicine

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Related Institution

Department of Critical Care Medicine, Changzhou Hospital of Traditional of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Department of Critical Care Medicine, Jiangsu Province Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Medical Examination Center, Hebei Province Hospital of Chinese Medicine

Department of Gynaecology and Obstetrics, Hebei Province Hospital of Chinese Medicine

Experimental Center for Teaching, Hebei Medical University

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Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge.

DOI：10.1007/s11655-017-2801-5

摘要

Abstract

关键词

Keywords

references