Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions

Miao Li; Ping Li; Mei Zhang; Feng Ma

doi:10.1007/s11655-017-2805-1

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Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions

Updated：2021-08-27

- Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions
- Chinese Journal of Integrative Medicine Vol. 24, Issue 1, Pages: 40-46(2018)
- Affiliations：
  
  1. Department of Geriatrics, the First Affiliated Hospital of Bengbu Medical College,Anhui Province,Bengbu,China
  2. Department of Chinese Medicine Tumor, the Affiliated Provincial Hospital of Anhui Medical University,Hefei,China
- Author bio：
- Funds：
  
  Supported by the State Administration of Traditional Chinese Medicine of China (2009-No.30)
- DOI：10.1007/s11655-017-2805-1
  CLC：
- Published：2018，
  
  Published Online：9 August 2017，
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Li, M., Li, P., Zhang, M. et al. Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions., Chin. J. Integr. Med. 24, 40–46 (2018). https://doi.org/10.1007/s11655-017-2805-1

Miao Li, Ping Li, Mei Zhang, et al. Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions. [J]. Chinese Journal of Integrative Medicine 24(1):40-46(2018)
Li, M., Li, P., Zhang, M. et al. Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions., Chin. J. Integr. Med. 24, 40–46 (2018). https://doi.org/10.1007/s11655-017-2805-1 DOI：

Miao Li, Ping Li, Mei Zhang, et al. Brucine suppresses breast cancer metastasis via inhibiting epithelial mesenchymal transition and matrix metalloproteinases expressions. [J]. Chinese Journal of Integrative Medicine 24(1):40-46(2018) DOI： 10.1007/s11655-017-2805-1.

摘要

To examine the effect of brucine on the migration

invasion

adhesion and expressions of epithelial-to-mesenchymal transition (EMT) markers and matrix metalloproteinases (MMPs) in the highly metastatic breast cancer cell lines MDA-MB-231 and Hs578-T. MDA-MB-231 and Hs578-T cells were divided to 4 groups: the control group (0.1% DMSO)

and 25

50 and 100 μmol/L brucine groups. The cell viability was determined using a CellTiter-Glo® luminescent cell viability. The scratch wound healing assay and tanswell migration assay were used to determine the migration ability of these cells treated by different concentrations of brucine. The proliferation rate

invasive potential and adhesive ability were respectively performed by colony formation assay

transwell invasion assay and adhension assay. The protein and mRNA expressions of EMT biomarkers

MMP-2 and MMP-9 were investigated by real-time reverse transcription polymerase chain reaction and Western blot. Compared with the control group

brucine had little effect on cell viability or proliferation (P>0.05)

but led to a dose-dependent decrease on migration

invasion

adhension of MDA-MB-231 and Hs578-T cells (P<0.01). Furthermore

brucine increased the protein and mRNA levels of EMT markers such as E-cadherin and β-catenin in MDA-MB-231 and Hs578-T cells

and decreased the protein and mRNA levels of mesenychmal markers such as vimentin and fibronectin

as well as the expressions of MMP-2 and MMP-9 (all P<0.01). Brucine inhibited triple negative breast cancer cells metastasis potentially through EMT reversion and MMP-2 and MMP-9 inhibition.

Abstract

To examine the effect of brucine on the migration

invasion

and 25

invasive potential and adhesive ability were respectively performed by colony formation assay

transwell invasion assay and adhension assay. The protein and mRNA expressions of EMT biomarkers

MMP-2 and MMP-9 were investigated by real-time reverse transcription polymerase chain reaction and Western blot. Compared with the control group

brucine had little effect on cell viability or proliferation (P>0.05)

but led to a dose-dependent decrease on migration

invasion

adhension of MDA-MB-231 and Hs578-T cells (P<0.01). Furthermore

brucine increased the protein and mRNA levels of EMT markers such as E-cadherin and β-catenin in MDA-MB-231 and Hs578-T cells

and decreased the protein and mRNA levels of mesenychmal markers such as vimentin and fibronectin

as well as the expressions of MMP-2 and MMP-9 (all P<0.01). Brucine inhibited triple negative breast cancer cells metastasis potentially through EMT reversion and MMP-2 and MMP-9 inhibition.

关键词

BrucineBreast Cancermetastasisepithelial mesenchymal transitionmatrix metalloproteinase

Keywords

BrucineBreast Cancermetastasisepithelial mesenchymal transitionmatrix metalloproteinase

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Department of Oncology, Affiliated Hospital of Bengbu Medical College

Department of Traditional Chinese Medicine Oncology, Provincial Hospital of Anhui Medical University

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Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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