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Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats
- Chinese Journal of Integrative Medicine Vol. 24, Issue 10, Pages: 758-762(2018)
- Affiliations：
1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine,Nanchang,China
2. Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University,Nanchang,China
3. Research Center for Differentiation and Development of Basic Theory of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine,Nanchang,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 81260523)
DOI：10.1007/s11655-017-2821-1
CLC：Published：2018，
Published Online：15 September 2017，
Scan for full text
Dong, W., Tang, Xl., Shang, Gb. et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats., Chin. J. Integr. Med. 24, 758–762 (2018). https://doi.org/10.1007/s11655-017-2821-1
Wei Dong, Xi-lan Tang, Guang-bin Shang, et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats. [J]. Chinese Journal of Integrative Medicine 24(10):758-762(2018)
Dong, W., Tang, Xl., Shang, Gb. et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats., Chin. J. Integr. Med. 24, 758–762 (2018). https://doi.org/10.1007/s11655-017-2821-1 DOI：
Wei Dong, Xi-lan Tang, Guang-bin Shang, et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats. [J]. Chinese Journal of Integrative Medicine 24(10):758-762(2018) DOI： 10.1007/s11655-017-2821-1.
摘要
To investigate the impact of dampness-heat (DH) on the development of mammary tumors in 7
12-dimethylbenz(a)anthracene (DMBA)-induced rats. Forty rats were randomly divided into 3 groups in a randomized block design
including the control group (n=13)
DMBA group (n=14)
and DMBA plus DH group (n=13). Rats in the DMBA group and DMBA plus DH group were intragastrically administrated with DMBA (100 mg/kg) for twice
once per week
while rats in the control group were treated with equivalent volumes of sesame oil. After DMBA administration
rats in the DMBA plus DH group were exposed to a simulated climate chamber with ambient temperature (33.0±0.5°C) and humidity (90%±5%) for 8 weeks
8 h per day. The body weight
time of tumor formation
and number of tumors were measured weekly to calculate tumor incidence
average latency period
average number of tumors
and average tumor weight. At the end of the experiment
the levels of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in serum
and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β in serum and tumor tissue were measured
respectively. Some tumor tissues were processed for hematoxylin-eosin staining to determine the histopathological changes. Compared with DMBA
DMBA plus DH significantly increased the average number of tumors
average tumor weight
levels of serum MMP-9
TIMP-1
TNF-α and IL-1β
and contents of tumor tissue TNF-α and IL-1β (P<0.05 or P<0.01). DH could accelerate the development of mammary tumors through increasing the expressions of MMP-9
TIMP-1
TNF-α and IL-1β in DMBA-induced rats.
Abstract
To investigate the impact of dampness-heat (DH) on the development of mammary tumors in 7
12-dimethylbenz(a)anthracene (DMBA)-induced rats. Forty rats were randomly divided into 3 groups in a randomized block design
including the control group (n=13)
DMBA group (n=14)
and DMBA plus DH group (n=13). Rats in the DMBA group and DMBA plus DH group were intragastrically administrated with DMBA (100 mg/kg) for twice
once per week
while rats in the control group were treated with equivalent volumes of sesame oil. After DMBA administration
rats in the DMBA plus DH group were exposed to a simulated climate chamber with ambient temperature (33.0±0.5°C) and humidity (90%±5%) for 8 weeks
8 h per day. The body weight
time of tumor formation
and number of tumors were measured weekly to calculate tumor incidence
average latency period
average number of tumors
and average tumor weight. At the end of the experiment
the levels of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in serum
and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β in serum and tumor tissue were measured
respectively. Some tumor tissues were processed for hematoxylin-eosin staining to determine the histopathological changes. Compared with DMBA
DMBA plus DH significantly increased the average number of tumors
average tumor weight
levels of serum MMP-9
TIMP-1
TNF-α and IL-1β
and contents of tumor tissue TNF-α and IL-1β (P<0.05 or P<0.01). DH could accelerate the development of mammary tumors through increasing the expressions of MMP-9
TIMP-1
TNF-α and IL-1β in DMBA-induced rats.
关键词
dampness-heatmammary tumor712-dimethylbenz(a)anthraceneinflammatory response
Keywords
dampness-heatmammary tumor712-dimethylbenz(a)anthraceneinflammatory response
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