Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes

Wan-tai Dang; Wen-guang Xie; Jing-guo Zhou

doi:10.1007/s11655-017-2826-9

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Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes

Updated：2021-08-27

- Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes
- Chinese Journal of Integrative Medicine Vol. 24, Issue 1, Pages: 24-31(2018)
- Affiliations：
  
  1. School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine,Chengdu,China
  2. Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong,Sichuan Province,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81603441), Educational Commission of Sichuan Province (No. 16ZA0282), and the China Postdoctoral Science Foundation (No. 2016M590872), China
- DOI：10.1007/s11655-017-2826-9
  CLC：
- Published：2018，
  
  Published Online：30 October 2017，
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Dang, Wt., Xie, Wg. & Zhou, Jg. Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes., Chin. J. Integr. Med. 24, 24–31 (2018). https://doi.org/10.1007/s11655-017-2826-9

Wan-tai Dang, Wen-guang Xie, Jing-guo Zhou. Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes. [J]. Chinese Journal of Integrative Medicine 24(1):24-31(2018)
Dang, Wt., Xie, Wg. & Zhou, Jg. Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes., Chin. J. Integr. Med. 24, 24–31 (2018). https://doi.org/10.1007/s11655-017-2826-9 DOI：

Wan-tai Dang, Wen-guang Xie, Jing-guo Zhou. Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes. [J]. Chinese Journal of Integrative Medicine 24(1):24-31(2018) DOI： 10.1007/s11655-017-2826-9.

摘要

To study the expression level and role of apoptosis-associated speck-like protein containing a caspase recruitment domain (PYCARD) gene transcript variant mRNA in peripheral blood mononuclear cells (PBMCs) of primary gout (PG) patients with different Chinese medicine (CM) syndromes. The expressions of PYCARD gene transcript variant mRNA and interleukin-1β (IL-1β) mRNA in PBMCs were investigated in 96 PG patients with acute phase (APPG

44 cases) and non-acute phase (NAPPG

52 cases) and 30 healthy controls (HCs) by reverse transcription-polymerase chain reaction (PCR) and/or realtime quantitative PCR. PYCARD and nuclear factor-κB (p50) [NF-κB (p50)] protein was detected by Western blot in PBMCs respectively. IL-1β

IL-4 and IL-10 protein levels in plasma of HCs and PG patients were measured by enzyme-linked immuno sorbent assay. The main CM syndromes in APPG patients were obstruction of dampness and heat syndrome (ODHS

36.36%) and intermingled phlegm-blood stasis syndrome (IPBSS

27.27%)

while in NAPPG patients were Pi (Spleen)-deficiency induced dampness syndrome (PDIDS

40.38%) and qi-blood deficiency syndrome (QBDS

26.92%). It showed statistical significances of the expressions of PYCARD gene and its transcript variant mRNA

the protein of PYCARD and NF-κB (p50) and the plasma IL-1β

IL-4 and IL-10 in APPG

NAPPG

ODHS

IPBSS

PDIDS and QBDS groups

compared with the HC group respectively (P<0.05 or P<0.01). There were also significant differences of mRNA expressions of PYCARD-1 and PYCARD-2 as well as protein expressions of IL-1β

IL-4 and IL-10 among the 4 CM syndromes groups (P<0.05 or P<0.01). Correlation analysis showed positive correlation between the mRNA expressions of PYCARD-1 gene transcript variant and IL-1β in APPG patients (r=0.3088

P=0.0183). PYCARD gene and its transcript variant may play a critical and regulative role in the inflflammatory response of PG patients with different phases and CM syndromes.

Abstract

44 cases) and non-acute phase (NAPPG

36.36%) and intermingled phlegm-blood stasis syndrome (IPBSS

27.27%)

while in NAPPG patients were Pi (Spleen)-deficiency induced dampness syndrome (PDIDS

40.38%) and qi-blood deficiency syndrome (QBDS

26.92%). It showed statistical significances of the expressions of PYCARD gene and its transcript variant mRNA

the protein of PYCARD and NF-κB (p50) and the plasma IL-1β

IL-4 and IL-10 in APPG

NAPPG

ODHS

IPBSS

PDIDS and QBDS groups

compared with the HC group respectively (P<0.05 or P<0.01). There were also significant differences of mRNA expressions of PYCARD-1 and PYCARD-2 as well as protein expressions of IL-1β

P=0.0183). PYCARD gene and its transcript variant may play a critical and regulative role in the inflflammatory response of PG patients with different phases and CM syndromes.

关键词

Chinese medicine syndromeprimary goutPYCARDgenetranscript variant

Keywords

Chinese medicine syndromeprimary goutPYCARDgenetranscript variant

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