Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma

Xiao-ming Liu; Yong-bin Wang; Qian Wu; Zhong-rui Bian; Xiao-wen Che

doi:10.1007/s11655-017-2830-0

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Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma

Updated：2021-08-27

- Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma
- Chinese Journal of Integrative Medicine Vol. 24, Issue 5, Pages: 353-358(2018)
- Affiliations：
  
  1. Department of Geriatric Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji’nan,China
  2. Department of Respiratory Medicine, the Second Hospital of Shandong University, Ji’nan,China
- Author bio：
- Funds：
  
  Supported by the Key Scientific and Technological Project of Shandong Province (No. 2014GSF119012), China
- DOI：10.1007/s11655-017-2830-0
  CLC：
- Published：2018，
  
  Published Online：30 October 2017，
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Liu, Xm., Wang, Yb., Wu, Q. et al. Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma., Chin. J. Integr. Med. 24, 353–358 (2018). https://doi.org/10.1007/s11655-017-2830-0

Xiao-ming Liu, Yong-bin Wang, Qian Wu, et al. Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma. [J]. Chinese Journal of Integrative Medicine 24(5):353-358(2018)
Liu, Xm., Wang, Yb., Wu, Q. et al. Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma., Chin. J. Integr. Med. 24, 353–358 (2018). https://doi.org/10.1007/s11655-017-2830-0 DOI：

Xiao-ming Liu, Yong-bin Wang, Qian Wu, et al. Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma. [J]. Chinese Journal of Integrative Medicine 24(5):353-358(2018) DOI： 10.1007/s11655-017-2830-0.

摘要

To investigate the effects of ligustrazine (LTZ) on airway inflammation in a mouse model of neutrophilic asthma (NA). Forty healthy C57BL/6 female mice were randomly divided into 4 groups using a random number table

including the normal control

LTZ and dexamethasone (DXM) groups

with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge

LTZ and DXM groups were intraperitoneally injected with LTZ (80 mg/kg) or DXM (0.5 mg/kg) for 14 d

respectively

while the other two groups were given the equal volume of normal saline. After last challenge for 24 h

the aerosol inhalation of methacholine was performed and the airway reactivity was measured. The bronchoalveolar lavage fluid (BALF) was collected. The Wright-Giemsa staining was used for total white blood cells and differential counts. The levels of cytokines interleukin (IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological change of lung tissue was observed by hematoxylin eosin staining. The airway responsiveness of the NA group was signifificantly higher than the normal control group (P<0.05)

while those in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05). The neutrophil and eosinophil counts in the LTZ and DXM groups were signifificantly lower than the NA group (P<0.05)

and those in the LTZ group were signifificantly lower than the DXM group (P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells in fifiltration in the NA group. The airway inflflammation in the LTZ and DXM groups were signifificantly alleviated than the NA group. The infifiltration in the LTZ group was signifificantly reduced than the DXM group. Compared with the normal control group

the IL-17 level in BALF was signifificantly increased and the IL-10 level in BALF was signifificantly decreased in the NA group (P<0.05). LTZ and DXM treatment signifificantly decreased IL-17 levels and increased IL-10 levels compared with the NA group (P<0.05)

and the changes in the above indices were more signifificant in the LTZ group (P<0.05). LTZ could alleviate the airway inflflammation in the NA mice model through increasing the IL-10 level and decreasing the IL-17 level.

Abstract

including the normal control

LTZ and dexamethasone (DXM) groups

with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge

LTZ and DXM groups were intraperitoneally injected with LTZ (80 mg/kg) or DXM (0.5 mg/kg) for 14 d

respectively

while the other two groups were given the equal volume of normal saline. After last challenge for 24 h

关键词

Ligustrazineneutrophilic asthmaairway inflflammationcytokine

Keywords

Ligustrazineneutrophilic asthmaairway inflflammationcytokine

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Department of Gerontology, Beijing Friendship Hospital, Capital Medical University

Library of Guangzhou University of Chinese Medicine

Hospital of Guangdong University of Technology

Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou University of Chinese Medicine

Department of Cerebropathia, the First Affiliated Hospital of Guangzhou University of Chinese Medicine

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