Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model

Yang Xu; Xiu-ping Chen; Feng Zhang; Hua-hua Hou; Jing-yi Zhang; Shu-xian Lin; An-sheng Sun

doi:10.1007/s11655-017-2900-3

Your Location：

Home >

Browse articles >

Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model

Updated：2021-08-27

- Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model
- Chinese Journal of Integrative Medicine Vol. 24, Issue 6, Pages: 429-435(2018)
- Affiliations：
  
  1. Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College,Zunyi,China
  2. Molecular Oncology Laboratory, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University,Shanghai,China
  3. Institute of Chinese Medical Sciences, University of Macau, Macau SAR,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-017-2900-3
  CLC：
- Published：2018，
  
  Published Online：1 September 2017，
Scan for full text
Xu, Y., Chen, Xp., Zhang, F. et al. Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model., Chin. J. Integr. Med. 24, 429–435 (2018). https://doi.org/10.1007/s11655-017-2900-3

Yang Xu, Xiu-ping Chen, Feng Zhang, et al. Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model. [J]. Chinese Journal of Integrative Medicine 24(6):429-435(2018)
Xu, Y., Chen, Xp., Zhang, F. et al. Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model., Chin. J. Integr. Med. 24, 429–435 (2018). https://doi.org/10.1007/s11655-017-2900-3 DOI：

Yang Xu, Xiu-ping Chen, Feng Zhang, et al. Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model. [J]. Chinese Journal of Integrative Medicine 24(6):429-435(2018) DOI： 10.1007/s11655-017-2900-3.

摘要

To investigate the effect and potential mechanisms of rutaecarpine (Rut) in a rat artery balloon-injury model. The intimal hyperplasia model was established by rubbing the endothelia with a balloon catheter in the common carotid artery (CCA) of rats. Fifty rats were randomly divided into five groups

ie. sham

model

Rut (25

50 and 75 mg/kg) with 10 rats of each group. The rats were treated with or without Rut (25

75 mg/kg) by intragastric administration for 14 consecutive days following injury. The morphological changes of the intima were evaluated by hematoxylin-eosin staining. The expressions of proliferating cell nuclear antigen (PCNA) and smooth muscle (SM) α-actin in the ateries were assayed by immunohistochemical staining. The mRNA expressions of c-myc

extracellular signal-regulated kinase 2 (ERK2)

MAPK phosphatase-1 (MKP-1) and endothelial nitric oxide synthase (eNOS) were determined by real-time reverse transcription-polymerase chain reaction. The protein expressions of MKP-1 and phosphorylated ERK2 (p-ERK2) were examined by Western blotting. The plasma contents of nitric oxide (NO) and cyclic guanosine 3'

5'-monophosphate (cGMP) were also determined. Compared with the model group

Rut treatment significantly decreased intimal thickening and ameliorated endothelial injury (P<0.05 or P<0.01). The positive expression rate of PCNA was decreased

while the expression rate of SM α-actin obviously increased in the vascular wall after Rut (50 and 75 mg/kg) administration (P<0.05 or P<0.01). Furthermore

the mRNA expressions of c-myc

ERK2 and PCNA were downregulated while the expressions of eNOS and MKP-1 were upregulated (P<0.05 or P<0.01). The protein expressions of MKP-1 and the phosphorylation of ERK2 were upregulated and downregulated after Rut (50 and 75 mg/kg) administration (P<0.05 or P<0.01)

respectively. In addition

Rut dramatically reversed balloon injury-induced decrease of NO and cGMP in the plasma (P<0.05 or P<0.01). Rut could inhibit the balloon injury-induced carotid intimal hyperplasia in rats

possibly mediated by promotion of NO production and inhibiting ERK2 signal transduction pathways.

Abstract

ie. sham

model

Rut (25

50 and 75 mg/kg) with 10 rats of each group. The rats were treated with or without Rut (25

extracellular signal-regulated kinase 2 (ERK2)

5'-monophosphate (cGMP) were also determined. Compared with the model group

Rut treatment significantly decreased intimal thickening and ameliorated endothelial injury (P<0.05 or P<0.01). The positive expression rate of PCNA was decreased

while the expression rate of SM α-actin obviously increased in the vascular wall after Rut (50 and 75 mg/kg) administration (P<0.05 or P<0.01). Furthermore

the mRNA expressions of c-myc

respectively. In addition

Rut dramatically reversed balloon injury-induced decrease of NO and cGMP in the plasma (P<0.05 or P<0.01). Rut could inhibit the balloon injury-induced carotid intimal hyperplasia in rats

possibly mediated by promotion of NO production and inhibiting ERK2 signal transduction pathways.

关键词

rutaecarpineballoon-injuryIntimal Hyperplasiaextracellular signal-regulated kinase 2Nitric Oxide

Keywords

rutaecarpineballoon-injuryIntimal Hyperplasiaextracellular signal-regulated kinase 2Nitric Oxide

references

Dangas G, Fuster V. Management of restenosis after coronary intervention. Am Heart J 1996;132:428–436.

Thiel WH, Esposito CL, Dickey DD, Dassie JP, Long ME, Adam J, et al. Smooth muscle cell-targeted RNA aptamer inhibits neointimal formation. Mol Ther 2016;24:779–787.

Wu X, Liu W, Jiang H, Chen J, Wang J, Zhu R, et al. Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling. Int J Mol Med 2016;37:436–444.

Yu PJ, Ferrari G, Pirelli L, Gulkarov I, Galloway AC, Mignatti P, et al. Vascular injury and modulation of MAPKs: a targeted approach to therapy of restenosis. Cell Signal 2007;19:1359–1371.

Li Y, McRobb LS, Khachigian LM. Inhibition of intimal thickening after vascular injury with a cocktail of vascular endothelial growth factor and cyclic Arg-Gly-Asp peptide. Int J Cardiol 2016;220:185–191.

Janssens S, Flaherty D, Nong Z, Varenne O, van Pelt N, Haustermans C, et al. Human endothelial nitric oxide synthase gene transfer inhibits vascular smooth muscle cell proliferation and neointima formation after balloon injury in rats. Circulation 1998;97:1274–1281.

Jia S, Hu C. Pharmacological effects of rutaecarpine as a cardiovascular protective agent. Molecules 2010;15:1873–1881.

Sheu JR, Hung WC, Lee YM, Yen MH. Mechanism of inhibition of platelet aggregation by rutaecarpine, an alkaloid isolated from Evodia rutaecarpa. Eur J Pharmacol 1996;318:469–475.

Li D, Peng J, Xin HY, Luo D, Zhang YS, Zhou Z, et al. Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats. Peptides 2008;29:1781–1788.

Sheu JR, Hung WC, Wu CH, Lee YM, Yen MH. Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments. Br J Haematol 2000;110:110–115.

Hu CP, Xiao L, Deng HW, Li YJ. The cardioprotection of rutaecarpine is mediated by endogenous calcitonin relatedgene peptide through activation of vanilloid receptors in guinea-pig hearts. Planta Med 2002;68:705–709.

Yi HH, Rang WQ, Deng PY, Hu CP, Liu GZ, Tan GS, et al. Protective effects of rutaecarpine in cardiac anaphylactic injury is mediated by CGRP. Planta Med 2004;70:1135–1139.

Li JZ, Peng J, Xiao L, Zhang YS, Liao MC, Li XH, et al. Reversal of isoprenaline-induced cardiac remodeling by rutaecarpine via stimulation of calcitonin gene-related peptide production. Can J Physiol Pharmacol 2010;88:949–959.

Wang GJ, Wu XC, Chen CF, Lin LC, Huang YT, Shan J, et al. Vasorelaxing action of rutaecarpine: effects of rutaecarpine on calcium channel activities in vascular endothelial and smooth muscle cells. J Pharmacol Exp Ther 1999;289:1237–1244.

Xu Y, Hou HH, Li Q, Zhang JY, Sun AS. Comparison of inhibitory effects of Wu Zhuyu alkaloids on proliferation of vascular smooth muscle cells in rat. Lishizhen Med Mater Res (Chin) 2013;24:2079–2080.

Li YJ, Zhang F, Gong QH, Wu Q, Yu LM, Sun AS. Rutaecarpine inhibits angiotensin II-induced proliferation in rat vascular smooth muscle cells. Chin J Integr Med 2014:20:682–687.

Gao Y, Deng J, Yu XF, Yang DL, Gong QH, Huang XN. Ginsenoside Rg1 inhibits vascular intimal hyperplasia in balloon-injured rat carotid artery by down-regulation of extracellular signal-regulated kinase 2. J Ethnopharmacol 2011;18;138:472–478.

Xu Y, Hou HH, Li Q, Zhang JY, Sun AS. Comparison of the anti-proliferative effect of alkaloids isolated from Wu-Zhu-Yu in rat vascular smooth muscle cells. Lishizhen Med Mater Res (Chin) 2013;24:2079–2080.

Moldovan GL, Pfander B, Jentsch S. PCNA, the maestro of the replication fork. Cell 2007;129:665–679.

Iatropoulos MJ, Williams GM. Proliferation markers. Exp Toxicol Pathol 1996;48:175–181.

Owens GK. Regulation of differentiation of vascular smooth muscle cells. Physiol Rev 1995;75:487–517.

Wang L, Zheng J, Du Y, Huang Y, Li J, Liu B, et al. Cartilage oligomeric matrix protein maintains the contractile phenotype of vascular smooth muscle cells by interacting with alpha(7)beta(1) integrin. Circ Res 2010;106:514–525.

Pei C, Qin S, Wang M, Zhang S. Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN. Mol Med Rep 2015;12:7310–7316.

Koyama H, Bornfeldt KE, Fukumoto S, Nishizawa Y. Molecular pathways of cyclic nucleotide-induced inhibition of arterial smooth muscle cell proliferation. J Cell Physiol 2001;186:1–10.

O'Connor DM, O'Brien T. Nitric oxide synthase gene therapy: progress and prospects. Expert Opin Biol Ther 2009;9:867–878.

Hogg ME, Varu VN, Vavra AK, Popowich DA, Banerjee MN, MartinezJ, et al. Effect of nitric oxide on neointimal hyperplasia based on sex and hormone status. Free Radic Biol Med 2011;50:1065–1074.

Shapiro PS, Ahn NG. Feedback regulation of raf-1 and mitogen-activated protein (MAP) kinase kinases 1 and 2 by map kinase phosphatase-1 (MKP-1). J Biol Chem 1998;273:1788–1793.

Lai K, Wang H, Lee WS, Jain MK, Lee ME, Haber E, et a1. Mitogen-activated protein kinase phosphatase-1 in rat arterial smooth muscle cell proliferation. J Clin Invest 1996;98:1560–1567.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats

Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis

Divergent immunomodulatory effects of extracts and phenolic compounds from the fern Osmunda japonica Thunb.

Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii

Related Author

No data

Related Institution

Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Department of Physiology, Bengbu Medical College

Tianjin University of Traditional Chinese Medicine

The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰