Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells

Ha-Rim Kim; Guem-San Lee; Mi-Seong Kim; Do-Gon Ryu; Hong-Seob So; Hyoung-Chul Moon; Young-Rae Lee; Sei-Hoon Yang; Kang-Beom Kwon

doi:10.1007/s11655-017-2922-x

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Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells

Updated：2021-08-27

- Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells
- Chinese Journal of Integrative Medicine Vol. 24, Issue 6, Pages: 436-441(2018)
- Affiliations：
  
  1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, South Korea
  2. Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk, South Korea
  3. Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan Jeonbuk, South Korea
  4. Institute of Customized Physical Therapy, Gwanju, South Korea
  5. Department of Oral Biochemistry, Wonkwang University School of Dentistry, Iksan, Jeonbuk, South Korea
  6. Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk, South Korea
- Author bio：
- Funds：
- DOI：10.1007/s11655-017-2922-x
  CLC：
- Published：2018，
  
  Published Online：15 January 2018，
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Kim, HR., Lee, GS., Kim, MS. et al. Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells., Chin. J. Integr. Med. 24, 436–441 (2018). https://doi.org/10.1007/s11655-017-2922-x

Ha-Rim Kim, Guem-San Lee, Mi-Seong Kim, et al. Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells. [J]. Chinese Journal of Integrative Medicine 24(6):436-441(2018)
Kim, HR., Lee, GS., Kim, MS. et al. Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells., Chin. J. Integr. Med. 24, 436–441 (2018). https://doi.org/10.1007/s11655-017-2922-x DOI：

Ha-Rim Kim, Guem-San Lee, Mi-Seong Kim, et al. Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells. [J]. Chinese Journal of Integrative Medicine 24(6):436-441(2018) DOI： 10.1007/s11655-017-2922-x.

摘要

To examinie the synergistic effects of Banxia Xiexin Decoction (半夏泻心汤

Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 human lung cancer cell lines. A549 cells were treated with varying concentrations (50–200 μg/mL) of cisplatin and BXDE alone or in combination for 96 h. We used 1-(4

5-dimethylthiazol-2-yl)-3

5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis

respectively. The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05)

which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V+/propidium iodide- stained cell population and a ladder pattern of discontinuous DNA fragments. Furthermore

the apoptosis was inhibited by the pan-caspase inhibitor

benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK). BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover

apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.

Abstract

To examinie the synergistic effects of Banxia Xiexin Decoction (半夏泻心汤

5-dimethylthiazol-2-yl)-3

5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis

respectively. The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05)

the apoptosis was inhibited by the pan-caspase inhibitor

benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK). BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover

apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.

关键词

Banxia Xiexin DecoctioncisplatinA549 cellsapoptosisanticancersynergismChinese Medicine

Keywords

Banxia Xiexin DecoctioncisplatinA549 cellsapoptosisanticancersynergismChinese Medicine

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