Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression

Ju-Yeon Park; Hyuck Kim; Dong-Woo Lim; Jai-Eun Kim; Won-Hwan Park; Sun-Dong Park

doi:10.1007/s11655-017-2923-9

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Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression

Updated：2021-08-27

- Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression
- Chinese Journal of Integrative Medicine Vol. 24, Issue 11, Pages: 860-866(2018)
- Affiliations：
  
  1. Institute of Korean Medicine, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea
  2. Department of Diagnostics, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea
  3. Department of Pathology, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea
  4. Department of Prescription, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea
- Author bio：
- Funds：
  
  Supported by Dongguk University Research Fund of 2015
- DOI：10.1007/s11655-017-2923-9
  CLC：
- Published：2018，
  
  Published Online：15 January 2018，
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Park, JY., Kim, H., Lim, DW. et al. Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression., Chin. J. Integr. Med. 24, 860–866 (2018). https://doi.org/10.1007/s11655-017-2923-9

Ju-Yeon Park, Hyuck Kim, Dong-Woo Lim, et al. Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression. [J]. Chinese Journal of Integrative Medicine 24(11):860-866(2018)
Park, JY., Kim, H., Lim, DW. et al. Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression., Chin. J. Integr. Med. 24, 860–866 (2018). https://doi.org/10.1007/s11655-017-2923-9 DOI：

Ju-Yeon Park, Hyuck Kim, Dong-Woo Lim, et al. Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression. [J]. Chinese Journal of Integrative Medicine 24(11):860-866(2018) DOI： 10.1007/s11655-017-2923-9.

摘要

To elucidate how ethanol extract of L. serratum (ELS) could exert anti-migratory effects on glioma with the suppression of nuclear factor kappa B (NF-κB) downstream pathway. Cell viability of ELS on C6 glioma was detected by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) assay and 2′

7′-dichlorofluorescin diacetate (DCFH-DA) assay were applied to measure NO production and reactive oxygen species (ROS) generation on lipopolysaccharide (LPS)-induced C6 glioma cells. NF-κB

mitogen-activated protein kinase (MAPK)

inducible nictric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein were determined by Western blot. Wound healing assay was used to investigate the inhibitory effect of ELS on fetal bovine serum (FBS)-induced migration and matrix metalloproteinase (MMP)-9 and -2 activity was examined by zymography. ELS suppressed LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK)

c-Jun N-terminal kinase (JNK)

and p38 through inhibiting the expression of chemokine CCL2 (or monocyte chemoattractant protein-1

MCP-1). In addition

ELS inhibited the expression of iNOS

COX-2

and the production of NO by LPS in C6 glioma cells. ELS also significantly decreased serum-induced migration of C6 glioma cells in scratch wound healing in a dose-dependent manner (P<0.01). The activity of MMP-9 and -2 were also significantly attenuated by ELS with LPS treatment (P<0.01). Our results suggest that downregulation of MMP-9 gene expression might be involved in the anti-migration effect of ELS against LPS-induced C6 glioma cells.

Abstract

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) assay and 2′

7′-dichlorofluorescin diacetate (DCFH-DA) assay were applied to measure NO production and reactive oxygen species (ROS) generation on lipopolysaccharide (LPS)-induced C6 glioma cells. NF-κB

mitogen-activated protein kinase (MAPK)

c-Jun N-terminal kinase (JNK)

and p38 through inhibiting the expression of chemokine CCL2 (or monocyte chemoattractant protein-1

MCP-1). In addition

ELS inhibited the expression of iNOS

COX-2

关键词

Lycopodium serratum Thunblipopolysaccharidematrix metalloproteinasemigrationC6 glioma cell

Keywords

Lycopodium serratum Thunblipopolysaccharidematrix metalloproteinasemigrationC6 glioma cell

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