<italic style="font-style: italic">Curcuma wenyujin</italic> Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell <italic style="font-style: italic">Helicobacter pylori</italic><sub>CagA+VacA+</sub> Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression

Hai-feng JIN; Jin-feng DAI; Li-na MENG; Bin LU

doi:10.1007/s11655-017-2958-y

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Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pylori_CagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression

Original Article | Updated：2021-08-23

- Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pylori_CagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression
- Chinese Journal of Integrative Medicine Vol. 26, Issue 2, Pages: 122-129(2020)
- Affiliations：
  
  Department of Gastrointerology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou (310006), China
- Author bio：
  
  Correspondence to: Prof. LU Bin, Tel: 86-571-86620285, Fax: 86-571-86620286, E-mail:Lvbin@medmail.com.cn
- Funds：
- DOI：10.1007/s11655-017-2958-y
  CLC：
- Published：01 February 2020，
  
  Published Online：17 August 2017，
  
  Accepted：06 December 2016
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Hai-feng JIN, Jin-feng DAI, Li-na MENG, et al. Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pylori_CagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression. [J]. Chinese Journal of Integrative Medicine 26(2):122-129(2020)
DOI：

Hai-feng JIN, Jin-feng DAI, Li-na MENG, et al. Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pylori_CagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression. [J]. Chinese Journal of Integrative Medicine 26(2):122-129(2020) DOI： 10.1007/s11655-017-2958-y.

摘要

Abstract

Objective:

To investigate the effects and possible mechanisms of action of

Curcuma wenyujin

Y. H. Chen et C. Ling n-Butyl alcohol extract (CWNAE) on repression of human gastric cancer (GC) AGS cell invasion induced by co-culturing with

Helicobacter pylori

(HP).

Methods:

AGS cells were cultured with HP of positive or negative cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) expression (CagA+/- or VacA+/-) and divided into 5 group. Group A was cultured without HP as a control

Group B with HP

CagA+VacA+

Group C with HP

CagA-VacA-

Group D with HP

CagA+VacA+

and CWNAE

and Group E with HP

CagA-VacA

and CWNAE. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) and tumor invasion assays

examinations of morphology and ultramicroscopic structures

quantitative real-time polymerase chain reaction and Western blots were performed to measure the effects and uncover the mechanisms behind these effects of HP

CagA+VacA+

and CWNAE on the epithelial-mesenchymal transition (EMT) of AGS cells.

Results:

The 10% inhibitory concentration of CWNAE against AGS cells after a 48 h incubation was 19.73±1.30 μg/mL. More AGS cells were elongated after co-culturing with HP

CagA+VacA+

than after culturing with HP

CagA-VacA-

. In tumor invasion assays

CagA+VacA+

significantly enhanced the invasiveness of AGS cells compared to the other experimental groups (all

value

0.05)

and this effect was inhibited by CWNAE. Treatment with CWNAE normalized tight junctions and reduced the number of pseudopodia of AGS cells co-cultured with HP

CagA+VacA+

. HP

CagA+VacA+

up-regulated zincfinger ebox binding homeobox 1 (ZEB1) in AGS cells after co-culturing for 24 h. Expression of caudal type homeobox transcription factor (CDX-2) and claudin-2 was significantly increased by HP

CagA+VacA+

(

0.05)

but not by HP

CagA-VacA-

Conclusions:

CagA+VacA+

promoted the invasiveness of AGS cells through up-regulation of ZEB1 transcription and claudin-2 and CDX-2 expression. CWNAE inhibited these effects of HP

CagA+VacA+

on AGS cells by down-regulating ZEB1 transcription

and CDX-2 and claudin-2 expression.

关键词

Keywords

Helicobacter pylorigastric cancerAGSzincfinger ebox binding homeobox 1caudal type homeobox transcription factorclaudin-2Chinese medicine

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Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pyloriCagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression

DOI：10.1007/s11655-017-2958-y

摘要

Abstract

关键词

Keywords

references

Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl Alcohol Extract Inhibits AGS Cell Helicobacter pylori_CagA+VacA+ Promoted Invasiveness by Down-Regulating Caudal Type Homeobox Transcription Factor and Claudin-2 Expression