Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

Shu-kun Zhang; Yu-zhen Zhuo; Cai-xia Li; Lei Yang; Hong-wei Gao; Xi-mo Wang

doi:10.1007/s11655-017-2959-x

Your Location：

Home >

Browse articles >

Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

Updated：2021-08-27

- Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury
- Chinese Journal of Integrative Medicine Vol. 24, Issue 4, Pages: 272-277(2018)
- Affiliations：
  
  1. Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Intergrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital,Tianjin,China
  2. Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical School,Boston,USA
  3. Department of Surgery, Tianjin Nankai Hospital,Tianjin,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-017-2959-x
  CLC：
- Published：2018，
  
  Published Online：17 May 2017，
Scan for full text
Zhang, Sk., Zhuo, Yz., Li, Cx. et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury., Chin. J. Integr. Med. 24, 272–277 (2018). https://doi.org/10.1007/s11655-017-2959-x

Shu-kun Zhang, Yu-zhen Zhuo, Cai-xia Li, et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury. [J]. Chinese Journal of Integrative Medicine 24(4):272-277(2018)
Zhang, Sk., Zhuo, Yz., Li, Cx. et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury., Chin. J. Integr. Med. 24, 272–277 (2018). https://doi.org/10.1007/s11655-017-2959-x DOI：

Shu-kun Zhang, Yu-zhen Zhuo, Cai-xia Li, et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury. [J]. Chinese Journal of Integrative Medicine 24(4):272-277(2018) DOI： 10.1007/s11655-017-2959-x.

摘要

To investigate the effect of combined application of Xuebijing Injection (血必净注射液

XBJ) and resolvin D1 (RvD1) on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. The cecal ligation and puncture (CLP) method was used to develop a mouse sepsis model. Specific pathogen free male C57BL/6 mice were randomly divided into 5 groups (n=20 each): sham

CLP

CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1. After surgery

mice in the CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight)

RvD1 (10 ng/g body weight)

and their combination (the same dose of XBJ and RvD1)

respectively. In each group

12 mice were used to observe 1-week survival rate

while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18

lung tissues were harvested for observing pathological changes

and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Compared with the CLP group

the histopathological damage of the lung tissues was mitigated

MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition

the 1-week survival rate was improved

proportion of CD18-expressing cells in whole blood and ICAM-1 protein expression in lung tissue were decreased in the CLP+XBJ+RvD1 group (P<0.05 or P<0.01). XBJ together with RvD1 could effectively inhibit leukocyte adhesion

reduce lung injury

and improve the survival rate of mice with sepsis.

Abstract

To investigate the effect of combined application of Xuebijing Injection (血必净注射液

CLP

CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1. After surgery

mice in the CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight)

RvD1 (10 ng/g body weight)

and their combination (the same dose of XBJ and RvD1)

respectively. In each group

12 mice were used to observe 1-week survival rate

while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18

lung tissues were harvested for observing pathological changes

and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Compared with the CLP group

the histopathological damage of the lung tissues was mitigated

MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition

the 1-week survival rate was improved

reduce lung injury

and improve the survival rate of mice with sepsis.

关键词

Xuebijing InjectionChinese Medicineresolvin D1sepsislung injury

Keywords

Xuebijing InjectionChinese Medicineresolvin D1sepsislung injury

references

Gustot T. Multiple organ failure in sepsis: prognosis and role of systemic inflammatory response. Curr Opin Crit Care 2011;17:153–159.

Martin GS, Mannino DM, Eaton S, Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003;348:1546–1154.

Bevilacqua MP, Nelson RM, Mannori G, Cecconi O. Endothelial-leukocyte adhesion molecules in human disease. Annu Rev Med 1994;45:361–378.

Gao X, Xu N, Sekosan M, Mehta D, Ma SY, Rahman A, Malik AB. Differential role of CD18 integrins in mediating lung neutrophil sequestration and increased microvascular permeability induced by Escherichia coli in mice. J Immunol 2001;167:2895–2901.

Itagaki K, Adibnia Y, Sun S, Zhao C, Sursal T, Chen Y, et al. Bacterial DNA induces pulmonary damage via TLR-9 through cross-talk with neutrophils. Shock 2011;36:548–552.

Xu N, Gao XP, Minshall RD, Rahman A, Malik AB. Timedependent reversal of sepsis-induced PMN uptake and lung vascular injury by expression of CD18 antagonist. Am J Physiol Lung Cell Mol Physiol 2002;282:L796–L802.

Zhao YJ, Yi WJ, Wan XJ, Wang J, Tao TZ, Li JB, et al. Blockade of ICAM-1 improves the outcome of polymicrobial sepsis via modulating neutrophil migration and reversing immunosuppression. Mediators Inflamm 2014;2014:195290.

Wang YX, Yao SL, Shu HQ, Liu H, Yuan SY, Chen XD, et al. Resolvin D1 attenuates lipopolysaccharide induced acute lung injury through CXCL-12/CXCR4 pathway. J Surg Res 2014;188:213–221.

Tong WQ and Zheng YS. Current status of Xuebijing Injection in treatment of respiratory diseases. Zhejiang J Integr Tradit Chin West Med (Chin) 2014;24:846–847.

Wang Q, Wu X, Tong X, Zhang Z, Xu B, Zhou W. Xuebijing ameliorates sepsis-induced lung injury by downregulating HMGB1 and RAGE expressions in mice. Evid Based Complement Alternat Med 2015;2015:860259.

Liu MW, Su MX, Zhang W, Wang YQ, Chen M, Wang L, et al. Protective effect of Xuebijing Injection on paraquat-induced pulmonary injury via down-regulating the expression of p38 MAPK in rats. BMC Complement Altern Med 2014;14:498.

Rittirsch D, Huber-Lang MS, Flierl MA, Ward PA. Immunodesign of experimental sepsis by cecal ligation and puncture. Nat Protoc 2009;4:31–36.

Wang Y, Roller J, Menger MD, Thorlacius H. Sepsisinduced leukocyte adhesion in the pulmonary microvasculature in vivo is mediated by CD11a and CD11b. Eur J Pharmacol 2013;702:135–141.

Burns AR, Takei F, Doerschuk CM. Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol 1994;153:3189–3198.

Hubbard AK, Thibodeau M, Giardina C. Cellular and molecular mechanisms regulating silica-induced adhesion molecule expression in mice. J Environ Pathol Toxicol Oncol 2001;20 Suppl 1:45–51.

Goodman RB, Pugin J, Lee JS, Matthay MA. Cytokinemediated inflammation in acute lung injury. Cytokine Growth Factor Rev 2003;14:523–535.

Matsukawa A, Hogaboam CM, Lukacs NW, Lincoln PM, Evanoff HL, Strieter RM, et al. Expression and contribution of endogenous IL-13 in an experimental model of sepsis. J Immunol 2000;164:2738–2744.

Norozian FM, Leoncio M, Torbati D, Meyer K, Raszynski A, Totapally BR. Therapeutic hypercapnia enhances the inflammatory response to endotoxin in the lung of spontaneously breathing rats. Crit Care Med 2011;39:1400–1406.

Zhu QG, Zhu HW. Blood stasis and microcirculation disturbance. Chin J Microcircul (Chin) 1994;4:43–45.

Yuan CR. Stasis microcirculation disturbance and stimulation of circulation. Res Tradit Chin Med (Chin) 1998;14(4):15–19.

Ma XJ, Yin HJ, Chen KY. Progress in correlation of blood stasis and inflammation. Chin J Integr Tradit West Med (Chin) 2007;27:669–672.

Liu MW, Wang YH, Qian CY, Li H. Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis. Int J Mol Med 2014;34:1492–1504.

Gao J, Kong L, Liu S, Feng Z, Shen H, Liu Q. Xuebijing Injection III Clinical Group. A prospective multicenter clinical study of Xuebijing Injection in the treatment of sepsis and multiple organ dysfunction syndrome. Chin Critic Care Med (Chin) 2015;27:465–470.

Hou SY, Feng XH, Lin CL, Tan YF. Efficacy of Xuebijing for coagulopathy in patients with sepsis. Saudi Med J 2015;36:164–169.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats

Xuebijing Injection (血必净注射液) increases early survival rate by alleviating pulmonary vasopermeability in rats subjected to severe burns

Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis

Efficacy of Xuebijing Injection (血必净注射液) on Cardiopulmonary Bypass-Associated Pulmonary Injury: A Prospective, Single-center, Randomized, Double Blinded Trial

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Related Author

No data

Related Institution

Department of Cerebropathia, the First Affiliated Hospital of Guangzhou University of Chinese Medicine

Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou University of Chinese Medicine

Hospital of Guangdong University of Technology

Library of Guangzhou University of Chinese Medicine

Medical School of Chinese PLA, Chinese PLA General Hospital

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰