Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells

Yu Zhang; li Hou; Xin-yi Chen

doi:10.1007/s11655-017-2963-1

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Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells

Updated：2021-08-27

- Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells
- Chinese Journal of Integrative Medicine Vol. 24, Issue 3, Pages: 213-217(2018)
- Affiliations：
  
  1. Department of Integrated Chinese and Western Medicine, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer Tianjing, Tianjing,China
  2. Key Laboratory of Cancer Prevention and Therapy,Tianjin,China
  3. Tianjin Clinical Research Center for Cancer,Tianjin,China
  4. Department of Hematology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-017-2963-1
  CLC：
- Published：2018，
  
  Published Online：6 October 2017，
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Zhang, Y., Hou, l. & Chen, Xy. Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells., Chin. J. Integr. Med. 24, 213–217 (2018). https://doi.org/10.1007/s11655-017-2963-1

Yu Zhang, li Hou, Xin-yi Chen. Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells. [J]. Chinese Journal of Integrative Medicine 24(3):213-217(2018)
Zhang, Y., Hou, l. & Chen, Xy. Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells., Chin. J. Integr. Med. 24, 213–217 (2018). https://doi.org/10.1007/s11655-017-2963-1 DOI：

Yu Zhang, li Hou, Xin-yi Chen. Effects of Compound Zhebei Granule (复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells. [J]. Chinese Journal of Integrative Medicine 24(3):213-217(2018) DOI： 10.1007/s11655-017-2963-1.

摘要

To investigate the effects of Compound Zhebei Granule (复方浙贝颗粒

CZG) combined with doxorubicin hydrochloride (adriamycin

ADM) on specific surface antigens in mice with KG-1a transplanted cells. A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flfl anks of BALB/c-nude mice. Twenty-four tumor bearing mice were divided into 4 groups according to a random number table

including normal saline control group

ADM group

high-dose CZG group

and mid-dose CZG group

with 6 mice in each group. Drug administration occurred on the 14th day after cell inoculation

and normal saline control group mice were gavaged with normal saline at 0.2 mL/10 g every other day. ADM group mice were intraperitoneally injected with ADM 1 mg/kg [conversion of adults

40 mg/(m2•d)] every other day. High- and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM (1 mg/kg) every other day. The administration period lasted for 10 days. The tumor xenografts were made into mononuclear cell suspensions after dissection

and the expressions of specific surface antigens

including CD34+CD38-

CD34+CD38-CD123+

CD34+CD38-CD96+ and CD34+CD38-CD33+

in KG-1a cell line tumor xenografts were detected by flfl ow cytometry. Compared with the control and ADM groups

expression of CD34+CD38- in the two CZG groups was significantly lower (P<0.05). Compared with the control group

expression of CD34+CD38-CD123+ in the two CZG groups decreased (P<0.05). The high-dose CZG group showed more obvious outcomes compared with the ADM group (P<0.05). Compared with the control and ADM groups

the expression of CD34+CD38-CD96+ and CD34+CD38-CD33+ in the two CZG groups decreased (P<0.05). CZG combined with doxorubicin could reduce the expression of CD34+CD38-

CD34+CD38-CD123+

CD34+CD38-CD96+ and CD34+CD38-CD33+ in KG-1a cell line tumor xenografts

which shows that CZG could target leukemia stem cells and play a role in chemosensitization.

Abstract

To investigate the effects of Compound Zhebei Granule (复方浙贝颗粒

CZG) combined with doxorubicin hydrochloride (adriamycin

including normal saline control group

ADM group

high-dose CZG group

and mid-dose CZG group

with 6 mice in each group. Drug administration occurred on the 14th day after cell inoculation

and normal saline control group mice were gavaged with normal saline at 0.2 mL/10 g every other day. ADM group mice were intraperitoneally injected with ADM 1 mg/kg [conversion of adults

and the expressions of specific surface antigens

including CD34+CD38-

CD34+CD38-CD123+

CD34+CD38-CD96+ and CD34+CD38-CD33+

in KG-1a cell line tumor xenografts were detected by flfl ow cytometry. Compared with the control and ADM groups

expression of CD34+CD38- in the two CZG groups was significantly lower (P<0.05). Compared with the control group

the expression of CD34+CD38-CD96+ and CD34+CD38-CD33+ in the two CZG groups decreased (P<0.05). CZG combined with doxorubicin could reduce the expression of CD34+CD38-

CD34+CD38-CD123+

CD34+CD38-CD96+ and CD34+CD38-CD33+ in KG-1a cell line tumor xenografts

which shows that CZG could target leukemia stem cells and play a role in chemosensitization.

关键词

acute myeloid leukemiaChinese Medicinedoxorubicinadriamycin

Keywords

acute myeloid leukemiaChinese Medicinedoxorubicinadriamycin

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