Objective:

2

To explore the possible underlying mechanism by investigating the effect of electroacupuncture (EA) treatment on the primary motor cortex and striatum in a unilateral 6-hydroxydopamine (6-OHDA) induced rat Parkinson's disease (PD) model.

Methods:

2

Male Sprague-Dawley rats were randomly divided into sham group (n=16)

model group (

n

=14)

and EA group (

n

=14). EA stimulation at Dazhui (GV 14) and Baihui (GV20) was applied to PD rats in the EA group for 4 weeks. Behavioral tests were conducted to evaluate the effectiveness of EA treatment. Metabolites were detected by 7.0 T proton nuclear magnetic resonance.

Results:

2

Following 4 weeks of EA treatment in PD model rats

the abnormal behavioral impairment induced by 6-OHDA was alleviated. In monitoring changes in metabolic activity

ratios of myoinositol/creatine (Cr) and N-acetyl aspartate (NAA)/Cr in the primary motor cortex were significantly lower at the injected side than the non-injected side in PD rats (

P

=0.024 and 0.020). The ratios of glutamate + glutamine (Glx)/Cr and NAA/Cr in the striatum were higher and lower

respectively

at the injected side than the non-injected side (

P

=0.046 and 0.008). EA treatment restored the balance of metabolic activity in the primary motor cortex and striatum. In addition

the taurine/Cr ratio and Glx/Cr ratio were elevated in the striatum of PD model rats compared to sham-lesioned rats (

P

=0.026 and 0.000). EA treatment alleviated the excessive glutamatergic transmission by down-regulating the striatal Glx/Cr ratio (

P

=0.001). The Glx/Cr ratio was negatively correlated with floor plane spontaneous locomotion in PD rats (

P

=0.027 and

P

=0.0007).

Conclusions:

2

EA treatment is able to normalize the metabolic balance in the primary motor cortex and striatum of PD rats

which may contribute to its therapeutic effect on motor deficits. The striatal Glx/Cr ratio may serve as a potential indicator of PD and a therapeutic target of EA treatment.