Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis

Qing-bing Zhou; Li-qi Wu; Yin Zhang; Zhi-fang Zhang; Feng-qin Xu

doi:10.1007/s11655-018-2561-x

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Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis

Updated：2021-08-27

- Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis
- Chinese Journal of Integrative Medicine Vol. 24, Issue 7, Pages: 512-517(2018)
- Affiliations：
  
  Institute of Geriatric Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81603490) and China Post Doctoral Science Foundation (No. 2016M590187)
- DOI：10.1007/s11655-018-2561-x
  CLC：
- Published：2018，
  
  Published Online：11 May 2018，
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Zhou, Qb., Wu, Lq., Zhang, Y. et al. Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis., Chin. J. Integr. Med. 24, 512–517 (2018). https://doi.org/10.1007/s11655-018-2561-x

Qing-bing Zhou, Li-qi Wu, Yin Zhang, et al. Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis. [J]. Chinese Journal of Integrative Medicine 24(7):512-517(2018)
Zhou, Qb., Wu, Lq., Zhang, Y. et al. Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis., Chin. J. Integr. Med. 24, 512–517 (2018). https://doi.org/10.1007/s11655-018-2561-x DOI：

Qing-bing Zhou, Li-qi Wu, Yin Zhang, et al. Effects of Zhizi Chuanxiong Capsule (栀子川芎胶囊) on the Abnormal Methylation in Rabbits with Atherosclerosis. [J]. Chinese Journal of Integrative Medicine 24(7):512-517(2018) DOI： 10.1007/s11655-018-2561-x.

摘要

To investigate the effects of Zhizi Chuanxiong Capsule (ZCC

栀子川芎胶囊) on abnormal DNA methylation in a rabbit model of atherosclerosis (AS). After 1 week of adaptive feeding

48 New Zealand white rabbits were randomly divided into 4 groups: a control group (n=12) fed with normal diet for 22 weeks; a model group (n=12) fed with high fat diet for 14 weeks followed by 8 weeks of normal diet feeding; a low-dose ZCC group (n=12) fed with high fat diet and low-dose ZCC for 14 weeks

followed by 8 weeks of normal diet and low-dose drug; a high-dose ZCC group (n=12) fed with high fat diet and high-dose drug for 14 weeks

followed by 8 weeks of normal diet and high-dose drug. After 22 weeks of feeding

blood samples were taken from the rabbit ear vein

and the genomic DNA was extracted for methylation immunoprecipitation sequencing (Medip-seq). The aorta tissues were collected for hematoxylin-eosin (HE) staining. Eight rabbits died during the feeding process. HE staining showed that the size of the lipid deposition on vessel wall and atherosclerotic plaque formation were reduced in both low- and high-dose group. The Medip-seq results showed that there were 146 abnormally methylated genes (including both hypermethylated gene and hypomethylated genes) in the model group

compared with the control group. Gene Ontology (GO) and Pathway analysis showed that these abnormally methylated genes were found to be involved in multiple AS-related functions and pathways

such as protein kinase C activity

cholesterol transport

mitogen-activated protein kinase (MAPK) signaling pathway

peroxisome proliferater-activated receptor signaling pathway

vascular smooth muscle contraction

inflammation and so on. The abnormal methylated genes in AS model group were altered in both low- and high-dose groups: low-dose ZCC could change 72 of the 146 abnormally methylated genes

high-dose ZCC could change 71. Through GO and Pathway analysis

these altered methylated genes were involved in protein kinase C activity

inflammatory pathway

MAPK signaling pathway

vascular endothelial growth factor signaling pathway

etc. ZCC could treat AS through regulating the abnormal hypermethylated and hypomethylated genes in AS rabbit model.

Abstract

To investigate the effects of Zhizi Chuanxiong Capsule (ZCC

栀子川芎胶囊) on abnormal DNA methylation in a rabbit model of atherosclerosis (AS). After 1 week of adaptive feeding

followed by 8 weeks of normal diet and low-dose drug; a high-dose ZCC group (n=12) fed with high fat diet and high-dose drug for 14 weeks

followed by 8 weeks of normal diet and high-dose drug. After 22 weeks of feeding

blood samples were taken from the rabbit ear vein

compared with the control group. Gene Ontology (GO) and Pathway analysis showed that these abnormally methylated genes were found to be involved in multiple AS-related functions and pathways

such as protein kinase C activity

cholesterol transport

mitogen-activated protein kinase (MAPK) signaling pathway

peroxisome proliferater-activated receptor signaling pathway

vascular smooth muscle contraction

inflammation and so on. The abnormal methylated genes in AS model group were altered in both low- and high-dose groups: low-dose ZCC could change 72 of the 146 abnormally methylated genes

high-dose ZCC could change 71. Through GO and Pathway analysis

these altered methylated genes were involved in protein kinase C activity

inflammatory pathway

MAPK signaling pathway

vascular endothelial growth factor signaling pathway

etc. ZCC could treat AS through regulating the abnormal hypermethylated and hypomethylated genes in AS rabbit model.

关键词

detoxifying and activating blood circulationatherosclerosismethylationstasis and toxinZhizi Chuanxiong capsule

Keywords

detoxifying and activating blood circulationatherosclerosismethylationstasis and toxinZhizi Chuanxiong capsule

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Department of Traditional Chinese Medicine, Shanghai No. 6 People’s Hospital, Affiliated to Shanghai Jiaotong University

Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou

Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University

Zhujiang Hospital, Southern Medical University

Integrative Hospital, College of Chinese Medicine, Southern Medical University

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