Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways

Xiao-feng Xu; Ru-bin Cheng; Xue-jin Zhang; Rui-lan Gao

doi:10.1007/s11655-018-2563-8

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Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways

Updated：2021-08-27

- Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways
- Chinese Journal of Integrative Medicine Vol. 24, Issue 12, Pages: 920-924(2018)
- Affiliations：
  
  1. Department of Hematology, Hangzhou Red Cross Hospital,Hangzhou,China
  2. College of Pharmaceutical Science, Zhejiang Chinese Medical University,Hangzhou,China
  3. The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by grants from Zhejiang Provincial Administration of Traditional Chinese Medicine (No. 2011ZA081, No. 2012ZB120, No. 2013ZB095 and No. 2014ZB089), Hangzhou Medical Science and Technology Plan (No. 2012A048)
- DOI：10.1007/s11655-018-2563-8
  CLC：
- Published：2018，
  
  Published Online：11 May 2018，
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Xu, Xf., Cheng, Rb., Zhang, Xj. et al. Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways., Chin. J. Integr. Med. 24, 920–924 (2018). https://doi.org/10.1007/s11655-018-2563-8

Xiao-feng Xu, Ru-bin Cheng, Xue-jin Zhang, et al. Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways. [J]. Chinese Journal of Integrative Medicine 24(12):920-924(2018)
Xu, Xf., Cheng, Rb., Zhang, Xj. et al. Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways., Chin. J. Integr. Med. 24, 920–924 (2018). https://doi.org/10.1007/s11655-018-2563-8 DOI：

Xiao-feng Xu, Ru-bin Cheng, Xue-jin Zhang, et al. Total Saponins of Rubus Parvifolius L. Exhibited Anti-Leukemia Effect in vivo through STAT3 and eIF4E Signaling Pathways. [J]. Chinese Journal of Integrative Medicine 24(12):920-924(2018) DOI： 10.1007/s11655-018-2563-8.

摘要

To investigate the anti-leukemia effect of total saponins of Rubus parvifolius L. (TSRP) on K562 cell xenografts in nude mice and the mechanisms of action. The K562 cell xenografts in nude mice were established

and then randomly divided into 5 groups

the control group

the cytosine arabinoside group(Ara-c) and 3 TSRP groups (20

40 and 100 mg/kg). The tumor volume and mass of each group of nude mice were measured and the anti-tumor rates of TSRP were calculated subsequently. The apoptosis status of tumor cells was detected by hematoxylin-eosin (HE) and terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining analysis. Finally

the activities of apoptosis related signaling of signal transducer and activator of transcription 3 (STAT3)

eukaryotic initiation factor 4E (eIF4E) and B-cell lymphoma-2 (bcl-2) were determined with immunohistochemistry tests. Subcutaneous injection of K562 cells induced tumor formation in nude mice

and the TSRP treated group showed a signifificant inhibitory effect on tumor formation. The nude mice treated with TSRP showed a signifificant decrease in tumor growth rate and tumor weight in comparison to the control group (all P<0.05). The HE staining and TUNEL assay showed that TSRP induced cell death by apoptosis. The immunohistochemical assay showed down-regulation of the bcl-2 gene in the TSRP treated cells. The phosphorylation levels of eIF4E and STAT3 were decreased obviously after the treatment of TSRP. TSRP had an excellent tumor-suppressing effect on K562 cells in the nude mice xenograft model

suggesting that TSPR can be developed as a promising anti-chronic myeloide leukemia drug.

Abstract

and then randomly divided into 5 groups

the control group

the cytosine arabinoside group(Ara-c) and 3 TSRP groups (20

the activities of apoptosis related signaling of signal transducer and activator of transcription 3 (STAT3)

eukaryotic initiation factor 4E (eIF4E) and B-cell lymphoma-2 (bcl-2) were determined with immunohistochemistry tests. Subcutaneous injection of K562 cells induced tumor formation in nude mice

suggesting that TSPR can be developed as a promising anti-chronic myeloide leukemia drug.

关键词

total saponins of Rubus parvifolius L.xenograft modelapoptosissignal transducer and activator of transcription 3eukaryotic initiation factor 4E

Keywords

total saponins of Rubus parvifolius L.xenograft modelapoptosissignal transducer and activator of transcription 3eukaryotic initiation factor 4E

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Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan

Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan

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