Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension

Yi-jia Shao; Jun Tao; Bing-bo Yu; Dan Meng; Xu-long Yang; Jia-pan Sun; Yan-xia Qiu; Xiao-yu Zhang

doi:10.1007/s11655-018-2568-3

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Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension

Updated：2021-08-27

- Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension
- Chinese Journal of Integrative Medicine Vol. 24, Issue 12, Pages: 897-904(2018)
- Affiliations：
  
  Department of Hypertension and Vascular Disease, the First Affiliated Hospital of Sun Yat-Sen University, Key Laboratory on Assisted Circulation, Ministry of Health,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the grants from the National Natural Science Foundation of China (No. 81500205, No. 31530023) and the Nature Science Foundation of Guangdong (No. 2016A030310184)
- DOI：10.1007/s11655-018-2568-3
  CLC：
- Published：2018，
  
  Published Online：19 October 2018，
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Shao, Yj., Tao, J., Yu, Bb. et al. Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension., Chin. J. Integr. Med. 24, 897–904 (2018). https://doi.org/10.1007/s11655-018-2568-3

Yi-jia Shao, Jun Tao, Bing-bo Yu, et al. Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension. [J]. Chinese Journal of Integrative Medicine 24(12):897-904(2018)
Shao, Yj., Tao, J., Yu, Bb. et al. Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension., Chin. J. Integr. Med. 24, 897–904 (2018). https://doi.org/10.1007/s11655-018-2568-3 DOI：

Yi-jia Shao, Jun Tao, Bing-bo Yu, et al. Berberine-Promoted CXCR4 Expression Accelerates Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Persons with Prehypertension. [J]. Chinese Journal of Integrative Medicine 24(12):897-904(2018) DOI： 10.1007/s11655-018-2568-3.

摘要

To evaluate whether the berberine treatment can improve endothelial repair capacity of early endothelial progenitor cells (EPCs) from prehypertensive subjects through increasing CXC chemokine receptor 4 (CXCR4) signaling. EPCs were isolated from prehypertensive and healthy subjects and cultured. In vivo reendothelialization capacity of EPCs from prehypertensive patients with or without in vitro berberine treatment was examined in a nude mouse model of carotid artery injury. The protein expressions of CXCR4/Janus kinase-2 (JAK-2) signaling of in vitro EPCs were detected by Western blot analysis. CXCR4 signaling and alteration in migration and adhesion functions of EPCs were evaluated. Basal CXCR4 expression was significantly reduced in EPCs from prehypertensive patients compared with normal subjects (P<0.01). Also

the phosphorylation of JAK-2 of EPCs

a CXCR4 downstream signaling

was significantly decreased (P<0.01). Berberine promoted CXCR4/JAK-2 signaling expression of in vitro EPCs (P<0.01). Transplantation of EPCs pretreated with berberine markedly accelerated in vivo reendothelialization (P<0.01). The increased in vitro function and in vivo reendothelialization capacity of EPCs were inhibited by CXCR4 neutralizing antibody or pretreatment with JAK-2 inhibitor AG490

respectively (P<0.01). Berberinemodified EPCs via up-regulation of CXCR4 signaling contributes to enhanced endothelial repair capacity in prehypertension

indicating that berberine may be used as a novel potential primary prevention means against prehypertension-related atherosclerotic cardiovascular disease.

Abstract

the phosphorylation of JAK-2 of EPCs

a CXCR4 downstream signaling

respectively (P<0.01). Berberinemodified EPCs via up-regulation of CXCR4 signaling contributes to enhanced endothelial repair capacity in prehypertension

indicating that berberine may be used as a novel potential primary prevention means against prehypertension-related atherosclerotic cardiovascular disease.

关键词

prehypertensionBerberineendothelial progenitor cellsreendothelializationCXC chemokine receptor 4

Keywords

prehypertensionBerberineendothelial progenitor cellsreendothelializationCXC chemokine receptor 4

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Department of Biochemistry, School of Medicine, Jinan University

Institute of Integrative Medicine, Jinan University

Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine, School of Medicine, Jinan University

Key Laboratory of Chinese Medicine on Viral Diseases, Ministry of Education, Microbiology and Immunology Department, Beijing University of Chinese Medicine

Guangdong Province Integrative Medicine Hospital

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