Objective:

2

To observe the effect and molecular mechanism of ethyl acetate extract of

Sceptridium ternatum

(STE) on the monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH).

Methods:

2

The main chemical components of

Sceptridium ternatum

were determined

and the effects in PAH rats were observed. A total of 140 Sprague Dawley rats were randomly and equally divided into the normal group

the model group

the Bosentan group

and the STE groups (2.5

5

10 g/kg) by the random number table method. The characteristic indicators of PAH were measured

and immunohistochemistry was used to observe the lung tissue of rats. Morphological changes of the lung tissue were observed under the light microscope.

Results:

2

Compared with the normal group

rats in the model group showed a significant increase in right ventricular free wall thickness (RVFWT)

mean pulmonary arterial pressure (mPAP)

mean right ventricular pressure (mRVP)

max right ventricular pressure (max RVP)

weight of right ventricle (RV)

and lung index (LI)

while a significant decrease in pulmonary artery acceleration time (PAAT

P

<

0.01). Compared with the model group

rats treated with STE had a significant decrease of RVFWT

mPAP

mRVP

max RVP

and RV

while a significant increase of PAAT (

P

<

0.01). After injection of MCT

nuclear factor-κB (NF-κB) p65 and α-smooth muscle actin (α-SMA) expression levels were up-regulated

and on the contrary

the treatment groups showed a significant downregulation without dose-dependent trend.

Conclusions:

2

STE can relieve the PAH in rats. STE may relieve pulmonary vascular disease and pulmonary injury by down-regulating the expression of NF-κB p65 and α-SMA.