Objective:

2

To decipher the possible mechanisms of

Sinomenium Acutum

(SA) in treating diseases by a bioinformatics method.

Methods:

2

SA ingredients were searched according to Chinese Pharmacopoeia

Chinese Medicine Dictionary and Traditional Chinese Medicines Database (TCMD). Active compounds and target proteins of SA were acquired through the Pubchem platform. Pathway

network and function analyses of SA were performed with ingenuity pathway analysis (IPA)

a bioinformatics analysis platform. Disease

biofunction-target networks were established with Cytoscape.

Results:

2

Eighteen ingredients from SA were obtained. Seven active ingredients with 31 active target proteins were acquired according to PubChem Bioassay test. By IPA analysis

277 canonical pathways belonging to 17 function categories were collected

23 kinds of diseases

21 categories bio-functions were obtained. Based on P value

calculated by IPA

the top 5 significant pathway of SA targets include phosphatidylinositol 3 kinase/Akt (PI3K/Akt) signaling

prostate cancer signaling

macrophage migration inhibitory factor (MIF) regulation of innate immunity

Guanosine-binding protein coupled receptor (GPCR) signaling

and ataxia telangiectasia mutated protein (ATM) signaling. Disease and bio-function network analysis indicated that mitogen activated protein kinase 1 (MAPK1)

MAPK3

p65 nuclear factor κB (RELA)

nuclear factor of κB inhibitor alpha (NFκBIA)

interleukin 1β(IL-1β)

prostaglandin G/H synthase 2 (PTGS2) and tumor protein 53 (TP53) were the critical targets in various diseases treated by SA.

Conclusions:

2

In the different view of target

pathway

disease and bio-function

inflammation was found to be a central theme in many chronic conditions. SA could be used not only as an anti-inflammatory agent

but also for the treatment of cancers

neurological diseases

psychological disorders and metabolic diseases.