Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats

Hu-qing Wang; Meng Zhang; Jia-xin Zhao; Hai-qin Wu; Zhen Gao; Gui-lian Zhang; Ru Zhang

doi:10.1007/s11655-018-2822-0

Your Location：

Home >

Browse articles >

Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats

Updated：2021-08-27

- Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats
- Chinese Journal of Integrative Medicine Vol. 24, Issue 5, Pages: 372-377(2018)
- Affiliations：
  
  Department of Neurology, the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81270415/H2501)
- DOI：10.1007/s11655-018-2822-0
  CLC：
- Published：2018，
  
  Published Online：9 January 2018，
Scan for full text
Wang, Hq., Zhang, M., Zhao, Jx. et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats., Chin. J. Integr. Med. 24, 372–377 (2018). https://doi.org/10.1007/s11655-018-2822-0

Hu-qing Wang, Meng Zhang, Jia-xin Zhao, et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats. [J]. Chinese Journal of Integrative Medicine 24(5):372-377(2018)
Wang, Hq., Zhang, M., Zhao, Jx. et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats., Chin. J. Integr. Med. 24, 372–377 (2018). https://doi.org/10.1007/s11655-018-2822-0 DOI：

Hu-qing Wang, Meng Zhang, Jia-xin Zhao, et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats. [J]. Chinese Journal of Integrative Medicine 24(5):372-377(2018) DOI： 10.1007/s11655-018-2822-0.

摘要

To observe the effect of puerarin on methyl-CpG binding protein 2 (MeCP2) phosphorylation (pMeCP2) in the hippocampus of a rat model of vascular dementia (VD). Thirty-six healthy Sprague-Dawley rats were randomly assigned to the sham-operated group

dementia group and puerarintreated group using a random number table (n=12 per group). The modifified permanent bilateral common carotid artery occlusion method was used to establish the VD model. The sham-operated and dementia groups were given 2 mL/d of saline

while the puerarin-treated group was given 100 mg/(kg•d) of puerarin for 17 days. The learning and memory abilities were evaluated by the Morris water maze test. Hematoxylin-eosin staining

immunohistochemical (IHC) staining and Western blot analysis were carried out to observe changes in neuron morphology and in level of pMeCP2 in the hippocampus

respectively. The morphologies of rat hippocampal neurons in the puerarintreated group were markedly improved compared with the dementia group. The escape latency of the dementia group was significantly longer than the sham-operated group (P<0.05)

while the puerarin-treated group was obviously shorter than the dementia group (P<0.05). Cross-platform times of the dementia group were signifificantly decreased compared with the sham-operated group (P<0.05)

while the puerarin-treated group was obviously increased compared with the dementia group (P<0.05). IHC staining showed no significant difference in the number of MeCP2 positive cells among 3 groups (P>0.05). The number of pMeCP2 positive cells in the CA1 region of hippocampus in the dementia group was signifificantly increased compared with the sham-operated group

and the puerarin-treated group was signifificantly increased compared with the dementia group (both P<0.05). Western blot analysis showed no signifificant difference of MeCP2 expression among 3 groups (P>0.05). The expression of pMeCP2 in the dementia group was signifificantly increased compared with the sham-operated group

while it in the puerarin-treated group was signifificantly increased compared with the dementia group (P<0.05). Puerarin could play a role in the protection of nerve cells through up-regulating pMeCP2 in the hippocampus

improving neuron morphologies

and enhancing learning and memory ablities in a rat model of VD.

Abstract

while the puerarin-treated group was given 100 mg/(kg•d) of puerarin for 17 days. The learning and memory abilities were evaluated by the Morris water maze test. Hematoxylin-eosin staining

immunohistochemical (IHC) staining and Western blot analysis were carried out to observe changes in neuron morphology and in level of pMeCP2 in the hippocampus

improving neuron morphologies

and enhancing learning and memory ablities in a rat model of VD.

关键词

Vascular DementiaPuerarinmethyl-CpG binding protein 2phosphorylation

Keywords

Vascular DementiaPuerarinmethyl-CpG binding protein 2phosphorylation

references

Sun GH. A review on the clinical application of puerarin. Clin J Chin Med (Chin) 2010;2:100–101.

Gao L, Ji X, Song J, Liu P, Yan F, Gong W, et al. Puerarin protects against ischemic brain injury in a rat model of transient focal ischemia. Neurol Res 2009;31:402–406.

Kong H, Wang XQ, Wang QG, Zhao Y, Sun Y, Zhang Y, et al. Effect of puerarin on the pharmacokinetics of baicalin in Gegen Qinlian Decoction in mice. Chin J Integr Med 2015. [Epub ahead of print]

Wu HQ, Wang HQ, Zhang B, Zhang GL, Zhang R, Zhang LF. Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats. Neur Regen Res 2012;7:421–425.

Zhang J, Guo WS, Tian BX, Sun MH, Li H, Zhou LN, et al. Puerarin attenuates cognitive dysfunction and oxidative stress in vascular dementia rats induced by chronic ischemia. Int J Clin Exp Pathol 2015;8:4695–4704.

Mcgraw CM, Samaco RC, Zoghbi HY. Adult neural function requires MeCP2. Science 2011;333:186–186.

Mcfarland KN, Huizenga MN, Darnell SB, Sangrey GR, Berezovska O, Cha JH, et al. MeCP2: a novel huntingtin interactor. Hum Mol Genet 2014;23:1036–1044.

Chen WG, Chang Q, Lin Y, Meissner A, West AE, Griffith EC, et al. Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. Science 2003;302:885–890.

Wang H, Chan SA, Ogier M, Hellard D, Wang Q, Smith C, et al. Dysregulation of brain-derived neurotrophic factor expression and neurosecretory function in MeCP2 null mice. J Neurosci 2006;26:10911–10915.

Lin Y, Savner E, Griffith EC, Hellard D, Wang Q, Smith C, et al. Brain-specific phosphorylation of MeCP2 regulates activity-dependent BDNF transcription, dendritic growth, and spinematuration. Neuron 2006;52:255–269.

de la Torre JC, Fortin T, Park GA, Butler KS, Kozlowski P, Pappas BA, et al. Chronic cerebrovascular insufficiency induces dementia-like deficits in aged rats. Brain Res 1992;582:186–195.

Wang Q, Li H, Wang FX, Gao L, Qin JC, Liu JG, et al. Huannao Yicong Decoction extract reduces inflammation and cell apoptosis in Aß1-42 -induced Alzheimer's disease model of rats. Chin J Integr Med 2017;23:672–680.

Zhang L, Lu L, Chan WM, Huang Y, Wai MS, Yew DT. Effects of DL-3-n-butylphthalide on vascular dementia and angiogenesis. Neurochem Res 2012;37:911–919.

Li P, Zhou YY. Methyl CpG-binding protein 2 participating in the regulation of differentiation plasticity of nerve regeneration in the basal ganglia after ischemic stroke. Chin J Contemp Neurol Neurosurg (Chin) 2013;13:955–966.

Tochiki KK, Cunningham J, Hunt SP, Géranton SM. The expression of spinal methyl-CpG-binding protein 2, DNA methyltransferases and histone deacetylases is modulated in persistent pain states. Mol Pain 2012;8:3267–3280.

Ohtani R, Tomimoto H, Kondo T, Wakita H, Akiguchi I, Shibasaki H, et al. Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia. Brain Res 2004;1023:31–40.

Wang XH, Li LS. Comparison between the two methods of establishing 2VO model in rat. Acta Academ Med Milit Tertiae (Chin) 2002;24:1496.

Zhao J, Gou YJ, Peng XL, Yan W, Xie SP, Fan C, et al. Donepezil in the treatment of senile vascular dementia: a systematic review. Chin J Evid Based Med 2011;11:1280–1289.

Doron R, Lotan D, Einat N, Yaffe R, Winer A, Marom I, et al. A novel herbal treatment reduces depressive-like behaviors and increases BDNF levels in the brain of stressed mice. Life Sci 2014;94:151–157.

Novkovic T, Mittmann T, Manahan-Vaughan D. BDNF contributes to the facilitation of hippocampal synaptic plasticity and learning enabled by environmental enrichment. Hippocampus 2015;25:1–15.

Nagahara AH, Tuszynski MH. Potential therapeutic uses of BDNF in neurological and psychiatric disorders. Nat Rev Drug Discov 2011;10:209–219.

Zhang BQ, Wang YL. Effcts of puerarin on hippocampal pyramidal cell and the expression of BDNF in vascular dementia rats. Chin J Neuroanat (Chin) 2007;23:615–620.

Marian M, Pinar A, Scott D, Skirmantas K, Nathaniel H. MeCP2 binds to 5HMC enriched within active genes and accessible chromatin in the nervous system. Cell 2012;151:1417–1430.

Cheng TL, Qiu Z. MeCP2: multifaceted roles in gene regulation and neural development. Neurosci Bull 2014;30:601–609.

Kishi N, Macklis JD. MeCP2 is progressively expressed in postmigratory neurons and is involved in neuronal maturation rather than cell fate decisions. Mol Cell Neurosci 2004;27:306–321.

Martinowich K, Hattori D, Wu H, Fouse S, He F, Hu Y, et al. DNA methylation-related chromatin remodeling in activitydependent BDNF gene regulation. Science 2003;302:890–893.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways

Panax notoginseng saponins induced up-regulation, phosphorylation and binding activity of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription factors in hematopoietic cells

In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation

Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils

Related Author

No data

Related Institution

Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine

The Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine

The First Hospital Affiliated to Tianjin University of Traditional Chinese Medicine

Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University

Deparment of Laboratory Medicine, Wenzhou Medical College

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰