Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation

Zi-gang Zhang; Xin Wang; Jin-hai Zai; Cai-hua Sun; Bing-chun Yan

doi:10.1007/s11655-018-2823-z

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Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation

Updated：2021-08-27

- Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation
- Chinese Journal of Integrative Medicine Vol. 24, Issue 5, Pages: 366-371(2018)
- Affiliations：
  
  1. Department of Neurology, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin,Jiangsu Province,China
  2. Department of Pathogeny Microbiology, Jiangsu University,Jiangsu Province,Zhenjiang,China
  3. Department of Rehabilitation, Northern Jiangsu People’s Hospital,Jiangsu Province,Yangzhou,China
  4. Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Jiangsu Province,Yangzhou,China
  5. Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,, Jiangsu Province,Jiangsu,Yangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81401005), the Natural Science Foundation of Jiangsu Province, China (No. BK20140494), and the University Natural Science Research Major Project of Jiangsu Province, China (No. 16KJA310006)
- DOI：10.1007/s11655-018-2823-z
  CLC：
- Published：2018，
  
  Published Online：9 January 2018，
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Zhang, Zg., Wang, X., Zai, Jh. et al. Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation., Chin. J. Integr. Med. 24, 366–371 (2018). https://doi.org/10.1007/s11655-018-2823-z

Zi-gang Zhang, Xin Wang, Jin-hai Zai, et al. Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation. [J]. Chinese Journal of Integrative Medicine 24(5):366-371(2018)
Zhang, Zg., Wang, X., Zai, Jh. et al. Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation., Chin. J. Integr. Med. 24, 366–371 (2018). https://doi.org/10.1007/s11655-018-2823-z DOI：

Zi-gang Zhang, Xin Wang, Jin-hai Zai, et al. Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation. [J]. Chinese Journal of Integrative Medicine 24(5):366-371(2018) DOI： 10.1007/s11655-018-2823-z.

摘要

To examine the effect of icariin (ICA) on the cognitive impairment induced by traumatic brain injury (TBI) in mice and the underlying mechanisms related to changes in hippocampal acetylation level. The modifified free-fall method was used to establish the TBI mouse model. Mice with post-TBI cognitive impairment were randomly divided into 3 groups using the randomised block method (n=7): TBI (vehicle-treated)

low-dose (75 mg/kg) and high-dose (150 mg/kg) of ICA groups. An additional sham-operated group (vehicle-treated) was employed. The vehicle or ICA was administrated by gavage for 28 consecutive days. The Morris water maze (MWM) test was conducted. Acetylcholine (ACh) content

mRNA and protein levels of choline acetyltransferase (ChAT)

and protein levels of acetylated H3 (Ac-H3) and Ac-H4 were detected in the hippocampus. Compared with the sham-operated group

the MWM performance

hippocampal ACh content

mRNA and protein levels of ChAT

and protein levels of Ac-H3 and Ac-H4 were signifificantly decreased in the TBI group (P<0.05). High-dose of ICA signifificantly ameliorated the TBI-induced weak MWM performance

increased hippocampal ACh content

and mRNA and protein levels of ChAT

as well as Ac-H3 protein level compared with the TBI group (P<0.05). ICA improved post-TBI cognitive impairment in mice by enhancing hippocampal acetylation

which improved hippocampal cholinergic function and ultimately improved cognition.

Abstract

mRNA and protein levels of choline acetyltransferase (ChAT)

and protein levels of acetylated H3 (Ac-H3) and Ac-H4 were detected in the hippocampus. Compared with the sham-operated group

the MWM performance

hippocampal ACh content

mRNA and protein levels of ChAT

and protein levels of Ac-H3 and Ac-H4 were signifificantly decreased in the TBI group (P<0.05). High-dose of ICA signifificantly ameliorated the TBI-induced weak MWM performance

increased hippocampal ACh content

and mRNA and protein levels of ChAT

as well as Ac-H3 protein level compared with the TBI group (P<0.05). ICA improved post-TBI cognitive impairment in mice by enhancing hippocampal acetylation

which improved hippocampal cholinergic function and ultimately improved cognition.

关键词

icariintraumatic brain injurycognitive impairmentacetylationhippocampus

Keywords

icariintraumatic brain injurycognitive impairmentacetylationhippocampus

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