FOLLOWUS
Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
- Chinese Journal of Integrative Medicine Vol. 24, Issue 10, Pages: 768-776(2018)
- Affiliations：
1. Institute of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital of Fudan University,Shanghai,China
2. The Academy of Integrative Medicine of Fudan University,Shanghai,China
3. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai,China
4. Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, the University of Tokyo,Tokyo,Japan
5. Department of Pharmacy, Kepler University Clinic, Neuromed Campus, Linz,Austria
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 31571196, 81401171, 30801502), the 2015 Program to Guide Medicine of the Shanghai Municipal Science and Technology Commission (No. 15401932200), the FY2008 JSPS Postdoctoral Fellowship for Foreign Researchers (No. P08471), the Shanghai Pujiang Program (No. 11PJ1401900), the Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine, the Program for Outstanding Medical Academic Leader, and Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine (No. 20150407)
DOI：10.1007/s11655-018-2835-3
CLC：Published：2018，
Published Online：17 April 2018，
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Zhang, B., Chu, N., Qiu, Xm. et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice., Chin. J. Integr. Med. 24, 768–776 (2018). https://doi.org/10.1007/s11655-018-2835-3
Bin Zhang, Nan Chu, Xue-min Qiu, et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice. [J]. Chinese Journal of Integrative Medicine 24(10):768-776(2018)
Zhang, B., Chu, N., Qiu, Xm. et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice., Chin. J. Integr. Med. 24, 768–776 (2018). https://doi.org/10.1007/s11655-018-2835-3 DOI：
Bin Zhang, Nan Chu, Xue-min Qiu, et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice. [J]. Chinese Journal of Integrative Medicine 24(10):768-776(2018) DOI： 10.1007/s11655-018-2835-3.
摘要
To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊
HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group)
including the control group (saline)
17β-estradiol group [E2
100 μg/(kg•d)]
and low-
medium-
and highdose of HYKT groups [0.3
0.9
2.7 g/(kg•d)
respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits
REC8 meiotic recombination protein (REC8)
structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT
the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γH2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles (P<0.05). Furthermore
it showed an increased trend in the levels of REC8 and SMC1β
after administration with E2 and HYKT
and no obvious change in the level of SMC3. HYKT could enhance the number of follicles
suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1β) in oocytes of aged mice
indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
Abstract
To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊
HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group)
including the control group (saline)
17β-estradiol group [E2
100 μg/(kg•d)]
and low-
medium-
and highdose of HYKT groups [0.3
0.9
2.7 g/(kg•d)
respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits
REC8 meiotic recombination protein (REC8)
structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT
the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γH2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles (P<0.05). Furthermore
it showed an increased trend in the levels of REC8 and SMC1β
after administration with E2 and HYKT
and no obvious change in the level of SMC3. HYKT could enhance the number of follicles
suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1β) in oocytes of aged mice
indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
关键词
Follicular DevelopmentcohesinoocyteHeyan Kuntai CapsuleChinese Medicine
Keywords
Follicular DevelopmentcohesinoocyteHeyan Kuntai CapsuleChinese Medicine
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